The tumor necrosis factor a of the bony fish seabream exhibits the in vivo proinflammatory and proliferative activities of its mammalian counterparts, yet it functions in a species-specific manner

García-Castillo, Jesús; Chaves-Pozo, Elena; Olivares, Pedro; Pelegrin, Pablo; Meseguer, José; Mulero Méndez, Victoriano Francisco

Publication:
The tumor necrosis factor a of the bony fish seabream exhibits the in vivo proinflammatory and proliferative activities of its mammalian counterparts, yet it functions in a species-specific manner

dc.contributor.author	García-Castillo, Jesús
dc.contributor.author	Chaves-Pozo, Elena
dc.contributor.author	Olivares, Pedro
dc.contributor.author	Pelegrin, Pablo
dc.contributor.author	Meseguer, José
dc.contributor.author	Mulero Méndez, Victoriano Francisco
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2024-01-30T11:07:16Z
dc.date.available	2024-01-30T11:07:16Z
dc.date.issued	2004
dc.description	©2004. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published version of a Published Work that appeared in final form in Cellular and Molecular Life Sciences. To access the final edited and published work see https://doi.org/10.1007/s00018-004-4068-1
dc.description.abstract	Information on the bioactivities of non-mammalian cytokines is scant due to the lack of the recombinant molecules and specific antibodies. We produced the mature predicted peptide of tumor necrosis factor a (TNFa) from the bony fish gilthead seabream (Sparus aurata L.) (sbTNFa), and its biological role was determined in vitro and in vivo. We first demonstrated by analytical size-exclusion chromatography that sbTNFa is an oligomeric protein but the dimer appears to predominate over the trimeric form, in contrast to mammalian TNFa. Intraperitoneal injection of native sbTNFa resulted in (i) priming of the respiratory burst of the peritoneal exudate and head-kidney (HK) leukocytes, the latter being the bone marrow equivalent in fish; (ii) rapid recruitment of phagocytic granulocytes to the injection site, and (iii) induction of granulopoiesis in the HK. Interestingly, sbTNFa was able to induce a strong proliferation of HK cells in vitro, whereas human TNFa did not. Conversely, sbTNFa was not cytotoxic for murine L929 fibroblasts.	es
dc.format	application/pdf	es
dc.format.extent	37	es
dc.identifier.citation	Cellular and Molecular Life Sciences, volumen 61, nº 11, año 2004, páginas 1331-1340.
dc.identifier.doi	10.1007/s00018-004-4068-1
dc.identifier.uri	http://hdl.handle.net/10201/138144
dc.language	eng	es
dc.publisher	Springer	es
dc.relation	Spanish Ministry of Science and Technology (BIO2001-2324-C02-02, AGL2002-03529, AGL2002-04306-C03-01), Spanish Ministry of Education, Culture and Sport, Fundación Séneca, Coordination Centre for Research (PI-51/00782/FS/01)	es
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s00018-004-4068-1	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Cytokine	es
dc.subject	TNFα	es
dc.subject	Fish	es
dc.subject	Sparus aurata	es
dc.subject.other	CDU::6 - Ciencias aplicadas	es
dc.title	The tumor necrosis factor a of the bony fish seabream exhibits the in vivo proinflammatory and proliferative activities of its mammalian counterparts, yet it functions in a species-specific manner	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	369f9ade-8896-4d91-84c5-e3f94a526070
relation.isAuthorOfPublication.latestForDiscovery	369f9ade-8896-4d91-84c5-e3f94a526070