Publication: Morphologic changes and methodological issues in the rabbit experimental model for diaphragmatic hernia
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Date
2010
Authors
Roubliova, Xenia I. ; Deprest, Jan A. ; Biard, Jean Marc ; Ophalvens, Lieve ; Gallot, Denis ; Jani, Jacques C. ; Van de Ven, Cornelis P. ; Tibboel, Dick ; Verbeken, Erik K.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Summary. Fetal lung development may be impaired by
some congenital anomalies or in utero events. Animal
models are used to understand the pathophysiology of
these diseases and explore therapeutic strategies. Our
group has an interest in the prenatal management of
congenital diaphragmatic hernia (CDH). Isolated CDH
remains associated with a 30% mortality because of lung
hypoplasia and pulmonary hypertension.
On day 23 of gestation (pseudoglandular stage)
CDH was created in both ovarian-end fetuses (n=28) in
14 time-mated pregnant white rabbits (hybrid of
Dendermonde and New-Zealand White). At term (day
30) all survived operated fetuses and size-matched
controls were harvested. Fetuses/lungs were assigned
randomly to formalin fixation either under pressure of 25
cm H2O (CDH25 n=5; CTR25 n=5) or without (0 cm
H2O (CDH0 n=7; CTR0 n=7). Fetuses and lungs were
first weighed, and then the lungs were processed for
morphometry. Pulmonary development was evaluated by
lung-to-body weight ratio (LBWR) and airway and
vascular morphometry.
Surgical induction of CDH does reduce the LBWR
to hypoplastic levels. The contralateral lung weight is
81% of what is expected, whereas the ipsilateral lung is
only 46% of the normal. This was accompagnied by a
loss of conducting airway generations, precisely,
terminal bronchioles (TB), which were surrounded by
less alveoli. The ipsilateral CDH lung demonstrated a
thickened media in the peripheral arteries as well. As a
result, in the severely hypoplastic ipsilateral lung, an
airway fixation pressure of 25 H2O has no significant
effect on the morphometric indices. The contralateral
lung has a normal amount of alveoli around a single TB,
which also behave like alveoli of the normal lung, i.e.
expand under pressure fixation.
The present study on severely hypoplastic lungs that
never respirated, shows that in contrast to normal lungs,
the morphometric indices are not significantly
influenced by a difference in fixation pressure.
Increasing fixation pressure seems to expand the lung
only when sufficient alveolated parenchyma is present.
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