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Domestic microwave processing for rapid immunohistochemical diagnosis of bovine rabies

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dc.contributor.authorAbreu, Camila C.
dc.contributor.authorNakayama, Priscilla A.
dc.contributor.authorNogueira, Clayton I.
dc.contributor.authorMesquita, Leonardo P.
dc.contributor.authorLopes, Priscila F.R.
dc.contributor.authorVaraschin, Mary S.
dc.contributor.authorSeixas, Josilene do N.
dc.contributor.authorFerreira, Enio
dc.contributor.authorBezerra Jr, Pedro S.
dc.date.accessioned2017-09-11T14:59:49Z
dc.date.available2017-09-11T14:59:49Z
dc.date.issued2012
dc.description.abstractThe present study describes the use of a microwave processing protocol for the rapid histopathological and immunohistochemical diagnosis of bovine rabies. Immunohistochemistry has been used for rabies diagnosis in formalin-fixed tissue with satisfactory results, although the time to diagnosis is considerably longer than that with direct immunofluorescence. The protocol provided a provisory histopathological rabies diagnosis in approximately three and half hours and the immunohistochemical diagnosis was available after six hours. The protocol achieved 100% correlation with direct immunofluorescence and is a promising method, particularly in situations in which only material in formalin is available for diagnosis or when the refrigeration or transportation of biological material is difficult.es
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dc.format.extent4es
dc.identifier.citationHistology and histopathology, Vol. 27, nº 9 (2012)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/54146
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectCattlees
dc.subjectHistopathologyes
dc.subject.otherCDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticoses
dc.titleDomestic microwave processing for rapid immunohistochemical diagnosis of bovine rabieses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.27, nº 9 (2012)