Publication: Domestic microwave processing for rapid immunohistochemical diagnosis of bovine rabies
Authors
Abreu, Camila C. ; Nakayama, Priscilla A. ; Nogueira, Clayton I. ; Mesquita, Leonardo P. ; Lopes, Priscila F.R. ; Varaschin, Mary S. ; Seixas, Josilene do N. ; Ferreira, Enio ; Bezerra Jr, Pedro S.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The present study describes the use of a microwave processing protocol for the rapid histopathological and immunohistochemical diagnosis of bovine rabies. Immunohistochemistry has been used for rabies diagnosis in formalin-fixed tissue with satisfactory results, although the time to diagnosis is considerably longer than that with direct immunofluorescence. The protocol provided a provisory histopathological rabies diagnosis in approximately three and half hours and the immunohistochemical diagnosis was available after six hours. The protocol achieved 100% correlation with direct immunofluorescence and is a promising method, particularly in situations in which only material in formalin is available for diagnosis or when the refrigeration or transportation of biological material is difficult.
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Citation
Histology and histopathology, Vol. 27, nº 9 (2012)
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