Publication: New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance
Authors
Franco-Martínez, Lorena ; Romero, Diego ; Peres Rubio, Camila ; Tecles, Fernando ; Martínez-Subiela, Silvia ; Teles, Mariana ; Tvarijonaviciute, Asta
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.cbpb.2018.04.006
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Description
© 2018 Elsevier Inc. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Comparative Biochemistry and Physiology, Part B. To access the final edited and published work see https://doi.org/10.1016/j.cbpb.2018.04.006
Abstract
The aim of the present report was to develop and validate new automated spectrophotometric assays for
measurement of total antioxidant capacity (TAC), thiols, and advanced oxidation protein products (AOPP) in
mussel gills, digestive gland and hemolymph samples, and to evaluate their possible utility in biomonitoring
programs. Total antioxidant capacity (TAC) was measured by different methods: trolox equivalent antioxidant
capacity (TEAC1 and TEAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of
plasma (FRAP). The assays were precise, accurate and provided low limits of detection. When oxidative stress
was promoted by inducing hypoxia and the behaviour of these biomarkers between hypoxic and controls mussels were compared, statistically significant differences were observed in all biomarkers and tissues evaluated. The results of the present study demonstrated that these biomarkers, not previously studied in mussels, show a potential use as biomarkers of oxidative stress in this species since they were validated and showed changes under a state of oxidative stress.
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Citation
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. Volumes 221–222, July 2018, Pages 44-49
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