Publication: Development of the small intestine of piglets in response to prenatal elevation of glucocorticoids
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Date
1991
Authors
Bate, L.A. ; Ireland, William P. ; Connell, B.J. ; Grimmelt, B.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The effects of prenatal adrenal stimulation
and synthetic glucocorticoicl supplementation on
developrncnt of the gastro-intestinal tract of the piglet
were investigated. Twelve pregnant sows were treated
with either ACTH infusion. Isoflupredone injection or
Saline between days I05 and 112 of gestation. Neonatal
pigs were weighed. bled and sacrificed at 0 or at 6 h.
Piglets sacrificed at 6 h were fed bovine colostrum.
Trailsvcrse sections were prcpared from the duucienuin.
i e j ~ ~ n uman cl ileum for measurcment of thc villus
amplification factor (VAF) and basal membrane
circunifercnce. Sows in the ACTH group showed an
cle\,ation in cortisol in response to infusion: this
decreased aftcr infusion and then rose again at
parturition. Piglcts from both the ACTH and Saline
groups had more villus surface area per unit of body
weight (BW) than those born to Isotluprcdone-treated
animals. The BW of the ACTH piglets was lowcr
(P < 0.05) than those of piglets in the other groups.
Whcn the weight of thc stomach and the Small Intestine
(SI) was cxpressetl as a function of thc body weight,
tlic stomach and S1:BW ratio was larger (p < 0.05) in
pigs born to ACTH-treated sows. The circumfcrence of
the ilcurn was larger at 6 h than at 0 h. Control pigs
had a higher concentration of bovine IgG at 4 and 6 h
(P < 0.05). Observations of thc light microscvpic
preparations indicated a less organized epithelium in
both ACTH and isotluprcdone pigs sacrificed at O Ii.
Light and EM preparations of ileum from ACTH pigs
sacrificed at 6 h. showcd an abundance of dark-stained
vacuoles. characteristic of IgG-containing structures.
These became lcss evident in piglets from the
Isotlupredone group and even less so in thc control
groups. The consequences of these phenomena in terms
of absorptive capacity are discusscd.
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