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Seeding of expanded polytetrafluoroethylene (ePTFE) vascular grafts. A morphological study of porcine endothelial and fibroblast cells

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dc.contributor.authorBuján, J.es
dc.contributor.authorBellón, J.M.es
dc.contributor.authorNavlet, J.G.
dc.contributor.authorHonduvilla, N.
dc.contributor.authorHernando, A.
dc.contributor.authorTuregano, F.
dc.date.accessioned2011-02-01T08:47:50Z
dc.date.available2011-02-01T08:47:50Z
dc.date.issued1992
dc.description.abstractThe need to improve clinical results with small and medium calibre grafts has led to extensive research on cell seeding of prosthetic materials. Numerous problems remain regarding identification, seeding, adhesion and survival of the cells attached. We have studied the behaviour of seedings of endothelial and fibroblast cells on ePTFE grafts. Scanning electron microscopy allows us to observe the morphological characteristics and their interaction with the biopolymers. It has been possible to differentiate both cellular types by their characteristics and interactions with the ePTFE. At the same time, from this ~ i vni t ro~st udy it can be concluded that the time needed to obtain a stable and confluent monolayer on ePTFE pretreated with fibronectin is between 18 hours to 4 days for endothelial cells, and 24 hours for fibroblasts. These would be the optimal time periods for ain vivo. grafting of the seeded prostheses.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/18351
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectEndothelial cellses
dc.subjectCell seedinges
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleSeeding of expanded polytetrafluoroethylene (ePTFE) vascular grafts. A morphological study of porcine endothelial and fibroblast cellses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol. 7, nº 4 (1992)