Publication: Seeding of expanded polytetrafluoroethylene (ePTFE) vascular grafts. A morphological study of porcine endothelial and fibroblast cells
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Date
1992
Authors
Buján, J. ; Bellón, J.M. ; Navlet, J.G. ; Honduvilla, N. ; Hernando, A. ; Turegano, F.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The need to improve clinical results with
small and medium calibre grafts has led to extensive
research on cell seeding of prosthetic materials.
Numerous problems remain regarding identification,
seeding, adhesion and survival of the cells attached.
We have studied the behaviour of seedings of
endothelial and fibroblast cells on ePTFE grafts.
Scanning electron microscopy allows us to observe
the morphological characteristics and their interaction
with the biopolymers. It has been possible to
differentiate both cellular types by their characteristics
and interactions with the ePTFE. At the same time, from
this ~ i vni t ro~st udy it can be concluded that the time
needed to obtain a stable and confluent monolayer on
ePTFE pretreated with fibronectin is between 18 hours
to 4 days for endothelial cells, and 24 hours for
fibroblasts. These would be the optimal time periods for
ain vivo. grafting of the seeded prostheses.
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