Publication: In vitro effects of estradiol on pituitary GH-immunoreactive cells
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Date
1997
Authors
Carretero, J. ; Rubio, M. ; Sanchez, F. ; Pereña, J. ; Estévez, F.I. ; Vázquez, G. ; Pérez, R.M. ; Vazquez, R.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In order to determine whether estradiol
modulates the proliferation and activity of somatotrophic
cells in vitro, a study of GH-immunoreactive cells was
carried out in pituitary monolayer cultures obtained from
male adult rats treated with ~ o - ~esMtrad iol for 3 hours.
Cellular activity was evaluated in a morphometric study
of GH-immunoreactive cells. The proliferation rate was
determined by double immunostaining for GH and
PCNA (proliferating cellular nuclear antigen). The
results were compared to those obtained from control
dishes. Estradiol was seen to increase the cellular
(p<0.05), nuclear (pc0.01) and cytoplasmic areas
(pc0.05). Estradiol decreased the percentage of
proliferating GH-immunroeactive cells (pc0.05) and the
numerical density of somatotrophs (pc0.05) when al1
cells present in the dishes were considered. However,
when only GH-immunoreactive cells were considered,
estradiol increased the proliferation rate of these cells
(pc0.05). Overall, our results suggest that, in vitro,
estradiol stimulates the cellular activity and proliferation
of GH-immunoreactive cells in the rat.
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