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In vitro effects of estradiol on pituitary GH-immunoreactive cells

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dc.contributor.authorCarretero, J.es
dc.contributor.authorRubio, M.es
dc.contributor.authorSanchez, F.
dc.contributor.authorPereña, J.
dc.contributor.authorEstévez, F.I.
dc.contributor.authorVázquez, G.
dc.contributor.authorPérez, R.M.
dc.contributor.authorVazquez, R.
dc.date.accessioned2011-02-22T10:41:36Z
dc.date.available2011-02-22T10:41:36Z
dc.date.issued1997
dc.description.abstractIn order to determine whether estradiol modulates the proliferation and activity of somatotrophic cells in vitro, a study of GH-immunoreactive cells was carried out in pituitary monolayer cultures obtained from male adult rats treated with ~ o - ~esMtrad iol for 3 hours. Cellular activity was evaluated in a morphometric study of GH-immunoreactive cells. The proliferation rate was determined by double immunostaining for GH and PCNA (proliferating cellular nuclear antigen). The results were compared to those obtained from control dishes. Estradiol was seen to increase the cellular (p<0.05), nuclear (pc0.01) and cytoplasmic areas (pc0.05). Estradiol decreased the percentage of proliferating GH-immunroeactive cells (pc0.05) and the numerical density of somatotrophs (pc0.05) when al1 cells present in the dishes were considered. However, when only GH-immunoreactive cells were considered, estradiol increased the proliferation rate of these cells (pc0.05). Overall, our results suggest that, in vitro, estradiol stimulates the cellular activity and proliferation of GH-immunoreactive cells in the rat.es
dc.formatapplication/pdfes
dc.format.extent6es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/18966
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectEstradioles
dc.subjectPituitaryes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleIn vitro effects of estradiol on pituitary GH-immunoreactive cellses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.12, nº 3 (1997)