Publication: Small and large extracellular vesicles of porcine seminal plasma differ in lipid profile
Authors
Martínez Diaz, Pablo ; Parra, Ana ; Sánchez López, Christian M. ; Casas, Josefina ; Lucas, Xiomara ; Marcilla, Antonio ; Roca, Jordi ; Barranco, Isabel
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Publisher
MDPI
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DOI
https://doi.org/10.3390/ijms25137492
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info:eu-repo/semantics/article
Description
© 2024 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in International Journal of Molecular Sciences. To access the final edited and published work see https://doi.org/10.3390/ijms25137492
Abstract
Seminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that remains poorly characterized. This study aimed to characterize the lipidomic profile of two subsets of differently sized sEVs, small (S-) and large (L-), isolated from porcine seminal plasma by size-exclusion chromatography and characterized by an orthogonal approach. High-performance liquid chromatography-high-resolution mass spectrometry was used for lipidomic analysis. A total of 157 lipid species from 14 lipid classes of 4 major categories (sphingolipids, glycerophospholipids, glycerolipids, and sterols) were identified. Qualitative differences were limited to two cholesteryl ester species present only in S-sEVs. L-sEVs had higher levels of all quantified lipid classes due to their larger membrane surface area. The distribution pattern was different, especially for sphingomyelins (more in S-sEVs) and ceramides (more in L-sEVs). In conclusion, this study reveals differences in the lipidomic profile of two subsets of porcine sEVs, suggesting that they differ in biogenesis and functionality.
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Citation
Int. J. Mol. Sci. 2024, 25(13), 7492
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