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Small and large extracellular vesicles of porcine seminal plasma differ in lipid profile

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dc.contributor.authorMartínez Diaz, Pablo
dc.contributor.authorParra, Ana
dc.contributor.authorSánchez López, Christian M.
dc.contributor.authorCasas, Josefina
dc.contributor.authorLucas, Xiomara
dc.contributor.authorMarcilla, Antonio
dc.contributor.authorRoca, Jordi
dc.contributor.authorBarranco, Isabel
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-09-04T10:20:10Z
dc.date.available2024-09-04T10:20:10Z
dc.date.issued2024-07-08
dc.description© 2024 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in International Journal of Molecular Sciences. To access the final edited and published work see https://doi.org/10.3390/ijms25137492
dc.description.abstractSeminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that remains poorly characterized. This study aimed to characterize the lipidomic profile of two subsets of differently sized sEVs, small (S-) and large (L-), isolated from porcine seminal plasma by size-exclusion chromatography and characterized by an orthogonal approach. High-performance liquid chromatography-high-resolution mass spectrometry was used for lipidomic analysis. A total of 157 lipid species from 14 lipid classes of 4 major categories (sphingolipids, glycerophospholipids, glycerolipids, and sterols) were identified. Qualitative differences were limited to two cholesteryl ester species present only in S-sEVs. L-sEVs had higher levels of all quantified lipid classes due to their larger membrane surface area. The distribution pattern was different, especially for sphingomyelins (more in S-sEVs) and ceramides (more in L-sEVs). In conclusion, this study reveals differences in the lipidomic profile of two subsets of porcine sEVs, suggesting that they differ in biogenesis and functionality.es
dc.formatapplication/pdfes
dc.format.extent18es
dc.identifier.citationInt. J. Mol. Sci. 2024, 25(13), 7492
dc.identifier.doihttps://doi.org/10.3390/ijms25137492
dc.identifier.issnPrint: 1661-6596
dc.identifier.issnElectronic: 1422-0067
dc.identifier.urihttp://hdl.handle.net/10201/143567
dc.languageenges
dc.publisherMDPI
dc.relationThis study was funded by grants: (1) PID2020-113493RB-I00 from the Spanish Ministry of Science and Innovation (MCIN/AEI/10.13039/501100011033), Madrid, Spain; (2) PID2022-137738NA-I00 from MCIN/AEI/10.13039/501100011033/FEDER EU; (3) RYC2021-034546-I funded by MCIN/AEI/10.13039/501100011033 and the European Union (NextgenerationEU/PRTR); and (4) 21935/PI/22 funded by the Seneca Foundation (Murcia, Spain).es
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/25/13/7492es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectExtracellular vesicleses
dc.subjectLipidomics
dc.subjectPorcine
dc.subjectSeminal plasma
dc.subjectSubsets
dc.titleSmall and large extracellular vesicles of porcine seminal plasma differ in lipid profilees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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