Browsing by Subject "Lipidomics"

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    Lipidomic analysis reveals alterations in hepatic FA profile associated with MASLD stage in patients with obesity
    (Oxford University Press, 2024-01-13) Núñez-Sánchez, María Ángeles; Martínez-Sánchez, María Antonia; Martínez-Montoro, José Ignacio; Balaguer-Román, Andrés; Murcia-García, Elena; Fernández Ruiz, Virginia Esperanza; Ferrer Gómez, Mercedes; Martínez Cáceres, Carlos Manuel; Sledzinski, Tomasz; Frutos Bernal, María Dolores; Hernández-Morante, Juan José; Fernández-García, José Carlos; Queipo-Ortuño, María Isabel; Ruiz Alcaraz, Antonio José; Mika, Adriana; Ramos-Molina, Bruno; Bioquímica y Biología Molecular B e Inmunología; Facultad de Biología
    Context. Metabolic dysfunction–associated steatotic liver disease (MASLD) is characterized by the intracellular lipid accumulation in hepatocytes. Excess caloric intake and high-fat diets are considered to significantly contribute to MASLD development. Objective. To evaluate the hepatic and serum fatty acid (FA) composition in patients with different stages of MASLD, and their relationship with FA dietary intake and MASLD-related risk factors. Methods. This was a case–control study in patients with obesity undergoing bariatric surgery at a university hospital between January 2020 and December 2021. Participants were distributed in 3 groups: no MASLD (n = 26), steatotic liver disease (n = 33), and metabolic dysfunction–associated steatohepatitis (n = 32). Hepatic and serum FA levels were determined by gas chromatography-mass spectrometry. Nutritional status was evaluated using validated food frequency questionnaires. The hepatic expression of genes involved in FA metabolism was analyzed by reverse transcription quantitative polymerase chain reaction. Results. The hepatic, but not serum, FA profiles were significantly altered in patients with MASLD compared with those without MASLD. No differences were observed in FA intake between the groups. Levels of C16:0, C18:1, and the C18:1/C18:0 ratio were higher, while C18:0 levels and C18:0/C16:0 ratio were lower in patients with MASLD, being significantly different between the 3 groups. Hepatic FA levels and ratios correlated with histopathological diagnosis and other MASLD-related parameters. The expression of genes involved in the FA metabolism was upregulated in patients with MASLD. Conclusion. Alterations in hepatic FA levels in MASLD patients were due to enhancement of de novo lipogenesis in the liver.
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    Small and large extracellular vesicles of porcine seminal plasma differ in lipid profile
    (MDPI, 2024-07-08) Martínez Diaz, Pablo; Parra, Ana; Sánchez López, Christian M.; Casas, Josefina; Lucas, Xiomara; Marcilla, Antonio; Roca, Jordi; Barranco, Isabel; Medicina y Cirugía Animal
    Seminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that remains poorly characterized. This study aimed to characterize the lipidomic profile of two subsets of differently sized sEVs, small (S-) and large (L-), isolated from porcine seminal plasma by size-exclusion chromatography and characterized by an orthogonal approach. High-performance liquid chromatography-high-resolution mass spectrometry was used for lipidomic analysis. A total of 157 lipid species from 14 lipid classes of 4 major categories (sphingolipids, glycerophospholipids, glycerolipids, and sterols) were identified. Qualitative differences were limited to two cholesteryl ester species present only in S-sEVs. L-sEVs had higher levels of all quantified lipid classes due to their larger membrane surface area. The distribution pattern was different, especially for sphingomyelins (more in S-sEVs) and ceramides (more in L-sEVs). In conclusion, this study reveals differences in the lipidomic profile of two subsets of porcine sEVs, suggesting that they differ in biogenesis and functionality.