Publication: Measurement of p-nitrophenyl acetate esterase activity (EA), total antioxidant capacity (TAC), total oxidant status (TOS) and acetylcholinesterase (AChE) in gills and digestive gland of Mytilus galloprovincialis exposed to binary mixtures of Pb, Cd and Cu
Authors
Franco-Martínez, Lorena ; Romero, Diego ; García-Navarro, José A. ; Tecles, Fernando ; Teles, Mariana ; Tvarijonaviciute, Asta
item.page.secondaryauthor
item.page.director
Publisher
Springer
publication.page.editor
publication.page.department
DOI
http://doi.org/10.1007/s11356-016-7677-y
item.page.type
info:eu-repo/semantics/article
Description
©2016 Springer. This document is the Published version of a Published Work that appeared in final form in Environmental Science and Pollution Research. To access the final edited and published work see https://doi.org/10.1007/s11356-016-7677-y
Abstract
The aims of the present work were (1) to evaluate oxidative stress biomarkers and AChE in two tissues of wild mussel (Mytilus galloprovincialis) of high biochemical activity and accumulation capacity (gills and digestive gland) and (2) to study the behaviour of these biomarkers in presence of heavy metals. For this, EA, TOS, TAC and AChE were measured in tissues of mussels exposed to binary combination of Pb, Cd and Cu. Mussels (n = 36) were exposed to one of the binary mixtures of Pb (1000 [mu]g L-1), Cd (100 [mu]g L-1) and Cu (100 [mu]g L-1) for 7 days, under controlled conditions. Gills and digestive gland were extracted and frozen at -80 °C until analysis. The automatic methods employed for the measurement of EA, TAC, TOS and AChE in M. galloprovincialis revealed higher levels of these biomarkers in digestive gland than gills. Study results suggest that gills would be the tissue of election for study oxidative stress markers, whereas digestive tissue should be selected for AChE measurements in case of evaluation of combined metal toxicity in mussels.
publication.page.subject
Citation
Environmental Science and Pollution Research, Vol. 23, N. 24, 2016, pp. 25385-25392
item.page.embargo
Collections
Ir a Estadísticas
Sin licencia Creative Commons.