Castells Mora, María Teresa

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Castells Mora, María Teresa

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Castells Mora, María Teresa

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Universidad de Murcia. Departamento de Biología Celular e Histología

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Open Access
Effect of antibiotics and NSAIDs on cyclooxygenase-2 in the enamel mineralization
(Nature Research, 2018-03-07) Serna Muñoz, Clara; Pérez Silva, Amparo; Solano, Francisco; Castells Mora, María Teresa; Vicente, Ascensión; Ortiz Ruiz, Antonio José; Dermatología, Estomatología, Radiología y Medicina Física
The objective of this study was to determine whether the use of the most commonly prescribed antibiotics and non-steroidal anti-inflammatory drugs in childhood could disturb enamel mineralization. Forty-two Swiss mice were divided into seven groups: controls; amoxicillin; amoxicillin/clavulanate; erythromycin; acetaminophen; ibuprofen and celecoxib, to inhibit cyclooxygenase 2 (COX2). SEM-EDX analysis was conducted on all cusps of the third molars. Calcium (Ca), phosphorus (P), aluminum, potassium, sodium, magnesium and chlorine were quantified. The stoichiometric Ca/P molar ratios were calculated. Immunohistochemical quantification of COX2 in incisors was carried out by image analysis using COX2-specific immunostaining. Groups treated with antibiotics showed no significant differences in the content of the chemical elements. Only acetaminophen and celecoxib showed a significant decrease in Ca and P compared with the control samples. Ca/P ratios showed no difference. Groups treated with amoxicillin, amoxicillin/clavulanate, erythromycin and acetaminophen showed significantly lower amounts of immunoreactive COX2 at the enamel organ maturation stage of the mouse incisors. Our results suggest that COX2 is involved in the maturation stage of the enamel organ and that its inhibition would appear to alter amelogenesis, producing hypomineralization.
Open Access
Uso de estatinas en biomodelos experimentales de arteriosclerosis
(Murcia: Universidad de Murcia, Servicio de Publicaciones, 2004) Ortega, J.V.; Ayala de la Peña, Ignacio; García Pérez, Bartolomé; Sánchez Polo, María Teresa; Castells Mora, María Teresa; Facultad de Veterinaria
Open Access
Modelos animales experimentales de enfermedad de hígado graso y síndrome metabólico
(Murcia : Universidad de Murcia, Servicio de Publicaciones, 2008) Ayala de la Peña, Ignacio; Cámara, P.; Fernández Pardo, Jacinto; Flores, I.; Cascales, A. I.; Gutiérrez Panizo, Cándido; Valdés, M; García Pérez, Bartolomé; Castells Mora, María Teresa; Facultad de Veterinaria
Open Access
Effects of atorvastatin on progression - regression of renal injury in hyperlipidemic chickens
(Murcia : F. Hernández, 2008) Adánez Martínez, María de Gracia; García Pérez, Bartolomé; Sánchez-Polo, M.T.; Montes, A.; Ayala de la Peña, Ignacio; Castells Mora, María Teresa; Martín Castillo, Antonia; Biología Celular; Medicina y Cirugía Animal
Complex interrelationships exist between hyperlipidemia and the progression of renal injury. The aim of this study was to evaluate the impact of high plasma cholesterol and triglyceride levels on renal structure and the effects of atorvastatin on progressionregression of renal injury. One-hundred chickens were divided into five groups: Group A: Standard diet (SD) for 6 months; Group B: Hyperlipidemic diet (HD) for 6 months; Group C: HD for three months and SD during the next 3 months; Group D: HD for 3 months and SD during the next 3 months, when they received oral atorvastatin (3 mg/kg/d); Group E: HD for the whole 6 months, and atorvastatin (3 mg/kg/d) during the last 3 months. Increased a-actine immunostaining was found in glomeruli of groups B and C. An important decrease of immunostaining was observed in glomeruli of atorvastatin treated groups. Group D showed the lowest value for presence of lipids, and significant differences were found with respect to the rest of the groups. The glomeruli of group B presented the highest damage grades and those of group D showed the lowest grades and presented significant differences from the rest of the groups. The combination of atorvastatin therapy and proper diet proved to be effective in promoting renal disease regression. However, the study of several parameters indicates that neither only diet nor atorvastatin in the progression group resulted completely effective in decreasing the progression of the disease.
Open Access
The effect of the flavonoid diosmin, grape seed extract and red wine on the pulmonary metastatic B16F10 melanoma
(Murcia : F. Hernández, 2005) Martínez, C.; Vicente García, Vicente; Yáñez, J.; Alcaraz, M.; Canteras, M.; Benavente-García García, Obdulio; Castillo, J.; Castells Mora, María Teresa
Objective: To study the effect of different phenolic compounds and red wine on pulmonary metastatic melanoma. Methods: Swiss mice were inoculated with 5x105 melanocytes B16F10 and given oral doses of diosmin, grape seed extract (GSE) and red wine. A macroscopic count was made of the metastatic nodules on the lung surface and a microscopic study by image analysis of five sections, calculating the implantation percentage and tumoral growth and invasion indices. Results: Macroscopically, the group treated with diosmin showed the greatest reduction (52%) in the number of metastatic nodules compared with the control group, which was treated with ethanol, while GSE and red wine caused decreases of 26.07 and 28.81%, respectively. Microscopically, there was a decrease in the implantation percentage after the administration of diosmin (79.4%) and red wine (20.19%), and an increase of 2.12% after the administration of GSE, all relative to the ethanol-treated control. As regards the growth index, diosmin produced a reduction of 67.44% and red wine a reduction of 20.62%, while GSE again produced an increase (25.33%). The reductions in the invasion index were 45.23, 31.65 and 17.57% with diosmin, GSE and red wine, respectively. Conclusions: Diosmin originated the greatest reduction in pulmonary metastases, both at the macroscopic and microscopic levels.
Open Access
Cytochemical and biochemical evidences for a complex tridimensional structure of the hamster zona pellucida
(Murcia : F. Hernández, 2009) Saavedra Leos, María Dolores; Gutiérrez Gallego, Ricardo; Fayrer Hosken, Richard; Ballesta Germán, José; Avilés Sánchez, Manuel; Castells Mora, María Teresa; Izquierdo Rico, María José; Jíménez Movilla, María; Martínez Alonso, Emma; Biología Celular
Zona pellucida (ZP) is an extracellular matrix that surrounds eggs and pre-implantation embryos and is required for in vivo fertility. A key event in successful fertilization is sperm binding to the surface of the ZP. It has been previously described that the hamster sperm binds mainly the outer region of the ZP which corresponds to the porous region in contact with the cumulus cells. Using ultrastructural cytochemistry approaches with an antibody developed against porcine ZP, this study shows that the pig ZP shares epitopes with some rodent species like hamster, rat and mouse. In the hamster, these epitopes are located mainly in the outer region of the ZP of preovulatory and ovulated oocytes. By means of biochemical approaches it was demonstrated that 1) the antibody is specific for the native hamster ZP3, 2) four different bands with a molecular weight of 67, 60, 48 and 38 kDa after Nlinked deglycosylation suggesting that the hamster ZP is formed by four proteins, and 3) the different composition observed in the outer region of the hamster ZP could be due to a specific supramolecular structure that makes some epitopes accessible for the antibodies. In summary, this study provides evidence that the different composition observed in the different regions of the ZP is mediated by a different organization of the components of the ZP produced during the oocyte maturation. This different organization could be responsible for the different sperm binding affinity observed for sperm to the outer region versus the inner region of the ZP.
Open Access
Estudio comparativo de diversos métodos de inducción de aterogénesis experimental en el pollo
(Murcia: Universidad de Murcia, Servicio de Publicaciones, 2004) Ayala de la Peña, Ignacio; García Pérez, Bartolomé; Doménech Asensi, Guillermo; Sánchez Polo, María Teresa; Ortega, J.V.; Castells Mora, María Teresa; Facultad de Veterinaria
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Caracterización histoquímica de las glucoproteínas del aparato respiratorio de diversos vertebrados / María Teresa Castells Mora ; directores Francisco Hernández Calvo, José Fco. ballesta Germán.
(Murcia : Universidad de Murcia, Departamento de Biología Celular,, 1989) Castells Mora, María Teresa
Open Access
Caracterización del pollo como biomodelo experimental en arteriosclerosis: lesiones en troncos supra-aórticos
(Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2011) Sánchez Polo, María Teresa; García Pérez, Bartolomé; Adánez Martínez, María de Gracia; Martin, A.; Ayala de la Peña, Ignacio; Castells Mora, María Teresa; Biología Celular; Medicina y Cirugía Animal
La enfermedad cardiovascular es hoy en día la primera causa de mortalidad en las sociedades desarrolladas. Dada la complejidad del desarrollo de la lesión aterosclerótica en el ser humano resulta interesante investigar en modelos animales en los que dicho proceso sea semejante a la enfermedad humana. El pollo, al igual que otras aves, es capaz de desarrollar arteriosclerosis aórtica y coronaria de forma natural o espontánea, e inducida por una dieta enriquecida en colesterol. Teniendo en cuenta que la mayoría de los trabajos publicados describen las lesiones en segmentos aórticos y la variedad de métodos de inducción de la arteriosclerosis, el objetivo de esta investigación es caracterizar de manera adecuada en el modelo aviar utilizado, las lesiones arterioscleróticas de troncos supra-aórticos en un grupo experimental con respecto a un grupo control. Se emplearon 20 pollos de la raza White Leghorn divididos en dos grupos (control y aterogénico) que recibían una dieta normal o hiperlipémica respectivamente durante un periodo de 6 meses. Se sacrificaron entonces los animales para llevar a cabo el estudio bioquímico del plasma (perfil lipídico), evaluación histológica de los troncos supra-aórticos y valoración semicuantitativa de las lesiones según la clasificación de Stary. Se observaron diferencias estadísticamente significativas entre ambos grupos para los diferentes parámetros bioquímicos estudiados y para la cuantificación del grado de lesión de Stary. En el grupo aterogénico se observó un endotelio conservado, con íntimas muy aumentadas de tamaño (10 veces el tamaño del grupo control) y muy desorganizadas. En conclusión, estos hallazgos confirman el uso del pollo como biomodelo experimental para el estudio de la arteriosclerosis en troncos supra-aórticos, y podrían ser empleados como referencia para futuros estudios intervencionistas.
Open Access
Hyperlipidemic chicken as a model of non-alcoholic steatohepatitis
(Frontiers Media, 2009-01-01) Ayala, Ignacio; Martin Castillo, Antonia; Adánez Martínez, María de Gracia; Fernández-Rufete Navarro, Ana; García Pérez, Bartolomé; Castells Mora, María Teresa; Medicina
Non-alcoholic steatohepatitis (NASH) is part of the spectrum of non-alcoholic fatty liver disease (NAFLD), currently the most common cause of abnormal liver tests. Given the difficulty of studying all the factors involved in it in human populations, studies in animal models might provide crucial insights in the pathogenesis of steatohepatitis. Several physiological features predispose birds to fat deposition in the liver. The present study was conceived to explore the possibilities of the chicken fed a cholesterol and fat enriched diet as a model for steatohepatitis. We used two different diets: a standard growing mash (control group) and a standard growing mash enriched with 2% cholesterol and 20% palm oil (hyperlipidemic group). We investigated the effect of feeding a cholesterol and fat enriched diet, on plasma lipid levels, liver enzymes and hepatic histopathology. Semiquantitative and quantitative assessment by image analysis was performed to determine changes in lipid deposits and inflammatory infiltration. Statistically significant increases were observed in all plasma lipid parameters, liver macroscopic features, fat deposits and cell-ballooning of hepatocytes between control and hyperlipidemic animals. Significant differences were also observed in the inflammatory infiltration parameters (number of foci, density, area and maximal diameter). Results show that diet-induced hypercholesterolemia and hypertriglyceridemia are associated with severe impairment of liver histology (fat accumulation, inflammation and cell-ballooning), reproducing histological features of human NAFLD. This model, which is easy and reproducible, offers economic and technical advantages. Furthermore, the reversibility of the pathologic changes makes it suitable for drug intervention studies of steatohepatitis.