Gómez Ramírez, Pilar

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Gómez Ramírez, Pilar

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Gómez Ramírez, Pilar

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Universidad de Murcia. Departamento de Ciencias Sociosanitarias

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Open Access
Prácticas en el laboratorio de Toxicología. Seguridad y normas en el laboratorio de Toxicología.
(2019-03-21) Gómez Ramírez, Pilar; Facultad de Medicina
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In vitro evaluation of cell death induced by cadmium, lead and their binary mixtures on erythrocytes of Common buzzard (Buteo buteo)
(Elsevier, 2013-11-25) Hernández-García, A.; Romero, D.; María-Mojica, P.; Martínez-López, E.; García Fernández, Antonio Juan; Gómez Ramírez, Pilar; Ciencias Sociosanitarias
Cadmium and lead are persistent and ubiquitous metals that can cause several deleterious effects in living beings. Apoptosis and necrosis are two types of cell death that can be found after in vivo and in vitro exposure to these metals. In this study, isolated red blood cells from living captive Common buzzard (Buteo buteo) were exposed in vitro to different concentrations of lead, cadmium, and the mixture lead–cadmium in a proportion of 1:10 (similar to that found in previous field studies). Data obtained from dose–response curves were used to evaluate the interactive effects of metal mixtures on cell viability. In general, except for the exposure to NOEC, additivity was the most frequently observed response. As described in human, after in vitro exposure, lead was highly accumulated in buzzard erythrocytes, while cadmium accumulation was scarce. Finally, the type of cell death (apoptosis or necrosis) induced by the exposure to different concentrations of these heavy metals and their mixtures was evaluated in the red blood cells. Apoptosis was found to be the main type of cell death observed after cadmium and/or lead exposure. However, this exposure caused an increase in lysis or necrosis, especially if red blood cells were exposed to high doses.
Open Access
Clase magistral Evaluación del riesgo de medicamentos en aguas de depuradoras
(2019-05-24) Gómez Ramírez, Pilar; Facultad de Medicina
Open Access
Heavy metals in tissues from loggerhead turtles (Caretta caretta) from the southwestern Mediterranean (Spain)
(Elsevier, 2009-02-02) Martínez-López, Emma; Hernández-García, Alejandro; María-Mojica, Pedro; Romero García, Diego; Castillo, Juan J.; Bellido, Juan J.; García Fernández, Antonio Juan; Gómez Ramírez, Pilar; Jiménez Montalbán, Pedro Javier; Ciencias Sociosanitarias
Cadmium, lead, copper, and zinc were measured in tissues of 21 loggerhead turtles (Caretta caretta) from the southwestern Mediterranean coastline. Mean concentrations (dry weight) of essential elements (Zn and Cu) were 107 and 21.6 μg/g in liver, 27.9 and 3.8 μg/g in kidney, 65.4 and 5.0 μg/g in pectoral muscle, 11.1 and 3.45 μg/g in brain, and finally 19.2 μg/g and undetected in bone, respectively. Mean concentrations of heavy metals (Cd and Pb) were 23.4 and 2.8 μg/g in liver, 31.5 and 0.5 μg/g in kidney, 0.2 and 0.3 μg/g in pectoral muscle, 0.2 and 0.7 μg/g in brain, and undetected and 1.2 μg/g in bone, respectively. Metal concentrations were similar to other studies conducted on Mediterranean turtles. However, cadmium concentrations varied widely among individuals, which has been associated with potential sources of cadmium in Mediterranean Sea. This is the first study into metal accumulation in tissues of loggerhead turtle from Spanish Mediterranean coastline.
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Spatiotemporal variations of organochlorine pesticides in an apex predator: Influence of government regulations and farming practices
(Elsevier, 2019-06-20) Pérez-García, Juan Manuel; León-Ortega, Mario; Enrique Martínez, José; Francisco Calvo, José; Sánchez-Zapata, José Antonio; Botella, Francisco; María-Mojica, Pedro; Martínez-López, Emma; García Fernández, Antonio Juan; Gómez Ramírez, Pilar; Ciencias Sociosanitarias
Background Intensification of agricultural practices has caused several negative effects to the environment. The use of fertilizers and pesticides may alter geochemical cycles or cause direct wildlife intoxication. Detrimental effects of organochlorine pesticides (OCPs) have forced the authorities to ban or restrict its use. This study evaluates the variation in levels of OCPs in a sentinel species in relation to changes in government regulations and the spatial configuration of agricultural practices around the nests. Methods Between 2003 and 2007, we analysed OCP levels in 256 blood samples of Eurasian Eagle-owl (Bubo bubo) chicks nesting in area of intensive commercial agriculture with historical frequent use of pesticides, in South-eastern Spain. We studied year-to-year variations in OCP concentrations and their relation with land use configuration around raptor nests by Generalized Linear Mixed Models (GLMM). Results OCPs were detected in 100% samples surveyed in 2003 and 2004, while dropped to 27% in 2005, 6.8% in 2006 and 6.3% in 2007, coinciding with the ban of OCPs. The presence of the main OCPs was related to agricultural practices. In particular, endosulfan and lindane were related to irrigated crops and urban areas, while DDT-related compounds and dieldrin were associated with dry land farming. Conclusions OCP concentrations in blood samples of Eurasian Eagle-owls may respond quickly to the implementations of new regulations about the use of agricultural products. This raptor was confirmed as a good sentinel species allowing rapid detection of changes in pesticides use.
Open Access
Evaluación del riesgo toxicológico: Toxicología, Grado en Farmacia
(2019-03-21) Gómez Ramírez, Pilar; Facultad de Medicina
Open Access
Validation of multi-residue method for quantification of antibiotics and nsaids in avian scavengers by using small amounts of plasma in HPLC-MS-TOF.
(MDPI, 2020-06-06) Blanco, Guillermo; García Fernández, Antonio Juan; Gómez Ramírez, Pilar; Ciencias Sociosanitarias
Pharmaceuticals are still considered emerging pollutants a ecting both aquatic and terrestrial ecosystems. Scavenging bird species may be exposed to veterinary drugs when they feed on livestock carcasses provided at supplementary feeding stations, as these are often stocked with ailing and/or recently medicated animals. Because those animals may be a source of several di erent pharmaceutical compounds, analytical methods to evaluate residue levels and exposure potential should enable detection and quantification of asmanydi erent compounds as possible, preferably from small sample volumes. Four di erent extraction methods were tested to conduct HPLC-MS-TOF analysis of some of the most common veterinary drugs used in livestock in Spain. The method deemed most viable was a simple extraction, using methanol and 100 L of plasma, that allowed quantification of seven antibiotics (tetracycline, oxytetracycline, ciprofloxacin, enrofloxacin, nalidixic acid, trimethoprim, sulfadiazine) and five nonsteroidal anti-inflammatory drugs (NSAIDs) (meloxicam, flunixin, carprofen, tolfenamic acid, phenylbutazone). The method was then applied to analysis of 29 Eurasian gri on vulture (Gyps fulvus) nestling samples, wherein enrofloxacin and tolfenamic acid were most commonly detected (69% and 20%, respectively). To our knowledge, this is the first study including NSAIDs in the exposure assessment of di erent classes of veterinary pharmaceuticals in live avian scavengers.
Open Access
Eurasian eagle owls Bubo Bubo adjust nest food stores to nestling age
(BioOne Digital Library, ) Puche Gómez, Sara; Perales Pacheco, Pablo; Botella, Francisco; Sánchez-Zapata, José Antonio; Pérez-García, Juan Manuel; Gómez Ramírez, Pilar; Ciencias Sociosanitarias
Some bird species, particularly owls, store prey within their nests during the breeding season. Food storage has been explained by the need to buffer the environmental variability of resources available for the offspring. This study describes food stores of the Eurasian Eagle Owl Bubo bubo in the southeast of the Iberian Peninsula and evaluates which intrinsic and extrinsic factors influence their presence and composition. Between 2003 and 2021, we studied the presence, abundance, biomass and species richness of prey in food stores found in 318 nests. Stores occurred in 63.5% of nests and included 520 prey items belonging to 23 different species. The main prey species was the European Rabbit oryctolagus cuniculus (71.43%). The greatest abundance of prey and biomass found in the stores occurred when the owlets were between 20 and 25 days old, that is when peak owlet growth occurs. Neither abundance nor biomass of prey in stores were related to brood size. Similarly, the frequency of food store occurrence in nests per year was unrelated to precipitation in the preceding months, which serves as a proxy of rabbit abundance. Globally these results support the hypothesis of that Eagle Owl parents adjust the stores according to when their owlets have their highest energy demands, irrespective of brood size and likely prey abundance. Food storage may help Eagle Owls to maximise their reproductive success in a semi-arid environment subject to high environmental variability.
Open Access
Evaluación del riesgo de los medicamentos para la salud humana: Toxicología, Grado en Farmacia
(2019-03-15) Gómez Ramírez, Pilar; Facultad de Medicina
Metadata only
Acumulación y distribución de metales pesados en tortuga boba (Caretta caretta) del mediterráneo suboccidental : tesina de la titulación de Veterinaria de la Universidad de Murcia / presentada por Pilar Gómez Ramírez
(Murcia : Universidad de Murcia, Facultad de Veterinaria, Grupo de Investigación de Toxicología y Veterinaria Forense,, 2006) Gómez Ramírez, Pilar