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Martínez Villanueva, Miriam

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Martínez Villanueva, Miriam
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Universidad de Murcia. Departamento de Bioquímica y Biología Molecular"B" e Inmunología
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    Self-reported DHA supplementation during pregnancy and its association with obesity or gestational diabetes in relation to DHA concentration in cord and maternal plasma: results from NELA, a prospective mother-offspring cohort.
    (2021-03) Gázquez, Antonio; Giménez-Bañón, María J.; Prieto-Sánchez, Maria T.; Martínez-Graciá, Carmen; Suárez, Clara; Morales, Eva; García-Marcos, Luis; Larqué, Elvira; Galdo-Castiñeira, Lina; Ballesteros-Meseguer, Carmen; Vioque, Jesús; Santaella-Pascual, Marina; Avilés Plaza, Francisco Valeriano; Martínez Villanueva, Miriam; Noguera Velasco, José Antonio; Cirugía, Pediatría y Obstetricia y Ginecología; Fisiología; Tecnología de Alimentos, Nutrición y Bromatología
    Maternal supplementation of docosahexaenoic acid (DHA) during pregnancy has been recommended due to its role in infant development, but its effect on materno-fetal DHA status is not well established. We evaluated the associations between DHA supplementation in pregnant women with obesity or gestational diabetes mellitus (GDM) and maternal and neonatal DHA status. Serum fatty acids (FA) were analyzed in 641 pregnant women (24 weeks of gestation) and in 345 venous and 166 arterial cord blood samples of participants of the NELA cohort. Obese women (n = 47) presented lower DHA in serum than those lean (n = 397) or overweight (n = 116) before pregnancy. Linoleic acid in arterial cord was elevated in obese women, which indicates lower fetal retention. Maternal DHA supplementation (200 mg/d) during pregnancy was associated with enhanced maternal and fetal DHA levels regardless of pre-pregnancy body mass index (BMI), although higher arterial DHA in overweight women indicated an attenuated response. Maternal DHA supplementation was not associated with cord venous DHA in neonates of mothers with GDM. The cord arteriovenous difference was similar for DHA between GDM and controls. In conclusion, maternal DHA supplementation during pregnancy enhanced fetal DHA status regardless of the pre-pregnancy BMI while GDM may reduce the effect of DHA supplementation in newborns.
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    Open Access
    Enfermedad ósea en el trasplante renal : marcadores bioquímicos de remodelado óseo y genotipado de polimorfismos
    (2012-06-26) Martínez Villanueva, Miriam; Martínez Hernández, Pedro; Tovar Zapata, Isabel; Gil del Castillo, María Luisa; Bioquímica y Biología Molecular B e Inmunología
    Los pacientes trasplantados renales presentan una rápida pérdida de masa ósea y mayor riesgo de fracturas en comparación con la población general. La osteoporosis es una enfermedad multifactorial en la que el componente genético puede influir hasta un 80% Se han estudiado durante dos años 139 pacientes trasplantados renales para evaluar la utilidad de los marcadores bioquímicos de remodelado óseo (MBRO) frente a la densitometría (DMO) como prueba estándar y los polimorfismos de genes del colágeno tipo 1 (Col1A1-spI), receptor de calcitonina (CTR-AluI), receptor de vitamina D (VDR-FokI, VDR-BsmI) y receptor de estrógenos (ESR-XbaI, ESR-PvuII) para evaluar su contribución a la pérdida de masa ósea. Se encuentra una disminución de DMO en columna lumbar y cuello femoral, MBRO correlacionan con DMO, polimorfismos de VDR-BsmI y ESR1-XbaI predisponen a una mayor pérdida de masa ósea, sugiriendo su utilidad en el diagnóstico precoz de enfermedad ósea postrasplante. Renal transplant patients show a rapid loss of bone mass and increased risk of fracture compared with the general population. Osteoporosis is a multifactorial skeletal disease in which genetic component may influence up to 80%. 139 kidney transplant patients have been studied for two years to evaluate the usefulness of biochemical markers of bone turnover (BMBT) versus densitometry (BMD) as standard test and to genotype polymorphisms of type 1 collagen (Col1A1-SpI), calcitonin receptor (CTR-AluI), vitamin D receptor (VDR-FokI, VDR-BsmI) and estrogen receptor (ESR-XbaI, ESR-PvuII), assessing their potential contribution to bone loss. A significant decrease of BMD in both lumbar spine and femoral neck was found, BMBT correlate with BMD, polymorphisms of VDRB-BsmI and ESR1-XbaI genes predispose to greater bone mass loss suggesting its usefulness in early diagnosis of bone disease.
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    Open Access
    Dietary Patterns in Pregnancy and Biomarkers of Oxidative Stress in Mothers and Offspring: The NELA Birth Cohort
    (2022-04) Morales, Eva; García-Serna, Azahara M.; Larqué, Elvira; Sánchez-Campillo, María; Serrano-Munera, Ana; Martinez-Graciá, Carmen; Suárez-Martínez, Clara; Vioque, Jesús; Ballesteros-Meseguer, Carmen; Galdo-Castiñeira, Lina; García-Marcos, Luis; Santaella-Pascual, Marina; Avilés Plaza, Francisco Valeriano; Martínez Villanueva, Miriam; Noguera Velasco, José Antonio; Ciencias Sociosanitarias
    Background: Although adherence to the Mediterranean and antioxidant-rich diets during pregnancy is suggested to improve maternal-fetal health by reducing oxidative stress, yet there is no study available. Objective: We examined whether maternal dietary patterns in pregnancy impact the biomarkers of oxidative stress in mothers and their offspring. Methods: Study population included 642 mothers and 335 newborns of the "Nutrition in Early Life and Asthma" (NELA) birth cohort. Maternal diet during pregnancy was assessed by a validated food frequency questionnaire and a priori-defined dietary indices (relative Mediterranean Diet [rMED], alternative Mediterranean Diet [aMED], Dietary Approach to Stop Hypertension [DASH], Alternate Healthy Index [AHEI], and AHEI-2010) were calculated. Biomarkers measured were: hydroperoxides, carbonyl groups, and 8-hydroxydeoxyguanosine (8OHdG) determined in maternal blood and newborn cord blood, and urinary maternal and offspring 15-F2t-isoprostane. Multivariate linear regression models were performed. Results: Maternal rMED score was inversely associated with the maternal levels of 8OHdG at mid-pregnancy (beta per 1-point increase = -1.61; 95% CI -2.82, -0.39) and the newborn levels of hydroperoxides (beta per 1-point increase = -4.54; 95% CI -9.32, 0.25). High vs. low maternal rMED score was marginally associated with the decreased levels of 8OHdG in newborns (beta = -9.17; 95% CI -19.9, 1.63; p for trend 0.079). Maternal DASH score tended to be inversely associated with maternal urinary 15-F2t-isoprostane (beta per 1-point increase = -0.69; 95% CI, -1.44, 0.06). High vs. low maternal AHEI score was associated with reduced offspring urinary levels of 15-F2t-isoprostane (beta = -20.2; 95% CI -38.0, -2.46; p for trend 0.026). Conclusion: These results suggest that maternal adherence to healthy dietary patterns during pregnancy may reduce DNA damage and lipid oxidation in mothers and offspring.
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    Lytic cell death induced by melittin bypasses pyroptosis but induces NLRP3 inflammasome activation and IL-1β release
    (Springer Nature, 2017-10-08) Martínez-García, Juan José; Muñoz-García, María; Andreu Martínez, David; Rivas, Luis; Martín Sánchez, María Rosario Fátima; Martínez Villanueva, Miriam; Noguera Velasco, José Antonio; Pelegrín Vivancos, Pablo; Farmacología
    The nucleotide-binding domain and leucine-rich repeat-containing receptor with a pyrin domain 3 (NLRP3) inflammasome is a sensor for different types of infections and alterations of homeostatic parameters, including abnormally high levels of the extracellular nucleotide ATP or crystallization of different metabolites. All NLRP3 activators trigger a similar intracellular pathway, where a decrease in intracellular K+ concentration and permeabilization of plasma membrane are key steps. Cationic amphipathic antimicrobial peptides and peptide toxins permeabilize the plasma membrane. In fact, some of them have been described to activate the NLRP3 inflammasome. Among them, the bee venom antimicrobial toxin peptide melittin is known to elicit an inflammatory reaction via the NLRP3 inflammasome in response to bee venom. Our study found that melittin induces canonical NLRP3 inflammasome activation by plasma membrane permeabilization and a reduction in the intracellular K+ concentration. Following melittin treatment, the apoptosis-associated speck-like protein, an adaptor protein with a caspase recruitment domain (ASC), was necessary to activate caspase-1 and induce IL-1β release. However, cell death induced by melittin prevented the formation of large ASC aggregates, amplification of caspase-1 activation, IL-18 release and execution of pyroptosis. Therefore, melittin-induced activation of the NLRP3 inflammasome results in an attenuated inflammasome response that does not result in caspase-1 dependent cell death.
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