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Elío Lucas, Patrícia

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Elío Lucas, Patrícia
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Universidad de Murcia. Departamento de Genética y Microbiología
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    The early injected genomic region determines sensitivity to Type I restriction-modification defence against Autographiviridae phages
    (Oxford University Press, 2025-10-14) Martínez Cazorla, Andrea; Martínez Jiménez, Christian José; Elío Lucas, Patrícia; Fineran, Peter C.; Jackson, Simon; Sánchez Amat, Antonio; Genética y Microbiología
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    A reverse transcriptase-Cas1 fusion protein contains a Cas6 domain required for both CRISPR RNA biogenesis and RNA spacer acquisition
    (Cell Press, 2018-11-15) Mohr, Georg; Silas, Sukrit; Stamos, Jennifer L.; Makarova, Kira S.; Markham, Laura M.; Yao, Jun; Elío Lucas, Patrícia; Sánchez Amat, Antonio; Fire, Andrew Z.; Koonin, Eugene V.; Lambowitz, Alan M.; Genética y Microbiología
    Prokaryotic CRISPR-Cas systems provide adaptive immunity by integrating portions of foreign nucleic acids (spacers) into genomic CRISPR arrays. Cas6 proteins then process CRISPR array transcripts into spacer-derived RNAs (CRISPR RNAs; crRNAs) that target Cas nucleases to matching invaders. We find that a Marinomonas mediterranea fusion protein combines three enzymatic domains (Cas6, reverse transcriptase [RT], and Cas1), which function in both crRNA biogenesis and spacer acquisition from RNA and DNA. We report a crystal structure of this divergent Cas6, identify amino acids required for Cas6 activity, show that the Cas6 domain is required for RT activity and RNA spacer acquisition, and demonstrate that CRISPR-repeat binding to Cas6 regulates RT activity. Co-evolution of putative interacting surfaces suggests a specific structural interaction between the Cas6 and RT domains, and phylogenetic analysis reveals repeated, stable association of free-standing Cas6s with CRISPR RTs in multiple microbial lineages, indicating that a functional interaction between these proteins preceded evolution of the fusion.
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    Type III CRISPR-Cas systems can provide redundancy to counteract viral escape from type I systems
    (eLife Sciences Publications, 2017-08-17) Silas, Sukrit; Elío Lucas, Patrícia; Jackson, Simon A.; Aroca Crevillén, Alejandra; Hansen, Loren L.; Fineran, Peter C.; Fire, Andrew Z.; Sánchez Amat, Antonio; Genética y Microbiología
    CRISPR-Cas-mediated defense utilizes information stored as spacers in CRISPR arrays to defend against genetic invaders. We define the mode of target interference and role in antiviral defense for two CRISPR-Cas systems in Marinomonas mediterranea. One system (type I-F) targets DNA. A second system (type III-B) is broadly capable of acquiring spacers in either orientation from RNA and DNA, and exhibits transcription-dependent DNA interference. Examining resistance to phages isolated from Mediterranean seagrass meadows, we found that the type III-B machinery co- opts type I-F CRISPR-RNAs. Sequencing and infectivity assessments of related bacterial and phage strains suggests an ‘arms race’ in which phage escape from the type I-F system can be overcome through use of type I-F spacers by a horizontally-acquired type III-B system. We propose that the phage-host arms race can drive selection for horizontal uptake and maintenance of promiscuous type III interference modules that supplement existing host type I CRISPR-Cas systems.
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