Publication: Type III CRISPR-Cas systems can provide redundancy to counteract viral escape from type I systems
Authors
Silas, Sukrit ; Elío Lucas, Patrícia ; Jackson, Simon A. ; Aroca Crevillén, Alejandra ; Hansen, Loren L. ; Fineran, Peter C. ; Fire, Andrew Z. ; Sánchez Amat, Antonio
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Publisher
eLife Sciences Publications
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DOI
https://doi.org/10.7554/eLife.27601.001
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info:eu-repo/semantics/article
Description
Abstract
CRISPR-Cas-mediated defense utilizes information stored as spacers in CRISPR arrays
to defend against genetic invaders. We define the mode of target interference and role in antiviral defense for two CRISPR-Cas systems in Marinomonas mediterranea. One system (type I-F) targets DNA. A second system (type III-B) is broadly capable of acquiring spacers in either orientation from RNA and DNA, and exhibits transcription-dependent DNA interference. Examining resistance to phages isolated from Mediterranean seagrass meadows, we found that the type III-B machinery co- opts type I-F CRISPR-RNAs. Sequencing and infectivity assessments of related bacterial and phage strains suggests an ‘arms race’ in which phage escape from the type I-F system can be overcome through use of type I-F spacers by a horizontally-acquired type III-B system. We propose that the
phage-host arms race can drive selection for horizontal uptake and maintenance of promiscuous type III interference modules that supplement existing host type I CRISPR-Cas systems.
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Citation
eLife, 2017, Vol. 6 : e27601
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