Ramírez Zarzosa, Gregorio José

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Ramírez Zarzosa, Gregorio José

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Ramírez Zarzosa, Gregorio José

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Anatomía y Anatomía Patológica Comparadas

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Open Access
Museo Anatómico Veterinario de la Universidad de Murcia:Gestión de su información para la planificación,organización, educación y acción cultural
(Murcia: Universidad de Murcia, Servicio de publicaciones, 2000) Granado, J. D.; Chaín Navarro, Celia; Vázquez Autón, José María; Gil Cano, Francisco; Latorre Reviriego, Rafael Manuel; López Albors, Octavio Miguel; Orenes Hernández, Mariano; Ramírez Zarzosa, Gregorio José; Facultad de Veterinaria
Open Access
Creation of three-dimensional anatomical vascular and biliary models for the study of the feline liver (Felis silvestris catus L.): a comparative CT, volume rendering (Vr), cast and 3D printing study
(MDPI, 2023-05-09) Rojo Ríos, Daniel; Ramírez Zarzosa, Gregorio José; Soler Laguía, Marta; Kilroy, David; Martínez Gomariz, Francisco; Sánchez Collado, Cayetano; Gil Cano, Francisco; García García, María I.; Raduán Jáber, José; Arencibia Espinosa, Alberto; Anatomía y Anatomía Patológica Comparada; Facultad de Veterinaria
In this study, six adult feline cadavers were examined using CTA, 3D printing, and casts injected with epoxy. The aorta, the portal vein, and the gallbladder of 3 feline cadavers were separately injected with a 50% mixture of colored vulcanized latex and hydrated barium sulfate as contrast medium to analyze by CT the arterial, venous and biliary systems. The other three cadavers were injected with a mixture of epoxy resin in the aorta, gallbladder and hepatic veins, separately. After the corrosion and washing process, hepatic vascular and biliary casts were obtained. The images obtained by CT showed the vascular and biliary system using a soft tissue window. For the identification of vascular and biliary structures, the 3D prints together with the 3D reconstructions were analyzed, and the results were compared with the casts obtained with epoxy resin. Each of the arterial, venous and biliary branches associated with each of the liver lobes were identified with the help of the printings. In conclusion, the creation of 3D prototypes of nonpathological feline hepatic parenchyma can be used in the veterinary clinic as a basis for the detection of pathological problems in addition to obtaining future pathological hepatic 3D models.
Open Access
Histopathological and immunophenotypical characterization of a combined melanoma and mucoepidermoid carcinoma in a dog
(Murcia, Universidad de Murcia, Servicio de Publicaciones, 2009) Martínez, M. C.; Sánchez, J.; Vilafranca, M.; Altimira, J.; García, B.; Buendía Marín, Antonio Julián; Navarro Cámara, José Antonio; Ramírez Zarzosa, Gregorio José; Facultad de Veterinaria
El presente trabajo describe el primer caso de un carcinoma mucoepidermoide canino pobremente diferenciado en el que se ha observado una proliferación maligna de melanocitos. La masa tumoral se localizó a nivel del 7º cuerpo vertebral en perro de raza mestiza. No se detectaron otros signos macroscópicos de carcinoma y/o melanoma oral o epitelial. El examen microscópico reveló la proliferación conjunta de ambas subpoblaciones. La proliferación y colonización de melanocitos en tumores de origen no melánico es rara y no se conoce la causa. En Patología humana, se han descrito casos de carcinomas mucoepidermoides pigmentados, pero en todos los casos se consideró la proliferación melanocítica como hiperplasia, no observándose en ningún caso signos de neoplasia. No existen en Patología humana ni veterinaria ningún caso descrito de carcinoma mucoepidermoide concomitante con un melanoma maligno, siendo éste el primer caso descrito.
Open Access
The histochemical profiles of fibre types in porcine skeletal muscle
(Murcia : F. Hernández, 2001) Vázquez, J.Mª; Moreno, F.; Gil Cano, Francisco; Latorre Reviriego, Rafael Manuel; López Albors, Octavio Miguel; Ramírez Zarzosa, Gregorio José
Using a variety of histochemical methods -mATPase staining after alkaline and acid preincubations, NADH-TR and a-MGPDH- we have investigated the fibre types in porcine skeletal muscle. The results reveal that four major fibre types -1, IIA, IIB and II*- can be separated histochemically in Longissimus lumborum muscle of Landrace pigs. The histochemical properties of the muscle fibre type 11* are very similar to that of type IIX described in other mammals. The existence of IIX fibres in pig muscle has been recently demonstrated by molecular biology techniques and our results validate the use of histochemistry (mATPase) as an easy methodology to differentiate the three fast myosins (type 11 fibres) in pig muscle.
Open Access
Anatomical and three-dimensional study of the female feline abdominal and pelvic vascular system using dissections, computed tomography angiography and magnetic resonance angiography
(MDPI, 2023-12-14) Rojo Ríos, Daniel; Soler Laguía, Marta; Kilroy, David; Sánchez Collado, Cayetano; García García, María I.; Arencibia Espinosa, Alberto; Ayala Florenciano, María Dolores; Gil Cano, Francisco; Martínez Gomariz, Francisco; Ramírez Zarzosa, Gregorio José; Sánchez Collado, Cayetano; Anatomía y Anatomía Patológica Comparadas; Facultad de Veterinaria
This study describes the anatomical characteristics of the abdominal and pelvic vascular system of two healthy mature female cats via three-dimensional contrast-enhanced computed tomography angiography, non-contrast enhanced magnetic resonance angiography and three-dimensional printing. Volume-rendering computed tomography angiography images were acquired from the ventral aspect using RadiAnt, Amira and OsiriX MD Dicom three-dimensional formats, and three-dimensional printing was obtained and compared with the corresponding computed tomography angiography images. Non-contrast enhanced magnetic resonance angiography was made us ing the time-of-flight imaging in ventral, oblique and lateral views. In addition, three cadavers with colored latex injection were dissected to facilitate the identification of the vascular structures. Three-dimensional computed tomography angiography showed the main vascular structures, whereas with the time-of-flight blood appeared with a high signal intensity compared with associated abdominal and pelvic tissues. Three-dimensional computed tomography angiography images and time-of-flight sequences provided adequate anatomical details of the main arteries and veins that could be used for future feline anatomical and clinical vascular studies of the abdomen and pelvis
Open Access
Estudio Histoquimico de la musculatura lateral de la carpa (Cyprinus Carpio)(1989)
(Universidad de Murcia, Servicio de publicaciones., 1989) Moreno Medina, Francisco; Vázquez Autón, José María; Gil Cano, Francisco; Latorre Reviriego, Rafael Manuel; Ramírez Zarzosa, Gregorio José; Facultad de Veterinaria
Open Access
A study of the head during prenatal and perinatal development of two fetuses and one newborn striped dolphin (Stenella coeruleoalba, Meyen 1833) using dissections, sectional anatomy, CT, and MRI: anatomical and functional implications in cetaceans and terrestrial mammals
(MDPI, 2019-12-13) Ríos y Loshuertos, Álvaro García de los; Arencibia Espinosa, Alberto; Soler Laguía, Marta; Gil Cano, Francisco; Martínez Gomariz, Francisco; López Fernández, Alfredo; Ramírez Zarzosa, Gregorio José; Anatomía y Anatomía Patológica Comparada; Facultad de Veterinaria
Our objective was to analyze the main anatomical structures of the dolphin head during its developmental stages. Most dolphin studies use only one fetal specimen due to the difficulty in obtaining these materials. Magnetic resonance imaging (MRI) and computed tomography (CT) of two fetuses (younger and older) and a perinatal specimen cadaver of striped dolphins were scanned. Only the older fetus was frozen and then was transversely cross-sectioned. In addition, gross dissections of the head were made on a perinatal and an adult specimen. In the oral cavity, only the mandible and maxilla teeth have started to erupt, while the most rostral teeth have not yet erupted. No salivary glands and masseter muscle were observed. The melon was well identified in CT/MRI images at early stages of development. CT and MRI images allowed observation of the maxillary sinus. The orbit and eyeball were analyzed and the absence of infraorbital rim together with the temporal process of the zygomatic bone holding periorbit were described. An enlarged auditory tube was identified using anatomical sections, CT, and MRI. We also compare the dolphin head anatomy with some mammals, trying to underline the anatomical and physiological changes and explain them from an ontogenic point of view.
Open Access
Anatomía veterinaria: experiencia metodológica en el marco del espacio europeo de educación superior
(Murcia, Universidad de Murcia, Servicio de Publicaciones, 2008) Vázquez Autón, José María; Ayala Florenciano, María Dolores; Gil Cano, Francisco; Latorre Reviriego, Rafael Manuel; López Albors, Octavio Miguel; Martínez Gomariz, Francisco; Ramírez Zarzosa, Gregorio José; Sánchez Collado, Cayetano; Facultad de Veterinaria
Open Access
A neuroanatomical study of the feline brain using MRI and mulligan staining: functional and pathological considerations
(2021) Diaz Martínez, E.; Ayala Florenciano, María Dolores; Arencibia Espinosa, A.; Soler Laguía, Marta; Kilroy, D.; Martínez Gomariz, Francisco; Ramírez Zarzosa, Gregorio José; Anatomía y Anatomía Patológica Comparada; Facultad de Veterinaria
Background: Despite multiple studies describing accurate diagnoses using advanced neuroimaging techniques, low and mid-field magnetic resonance imaging (MRI) are still the most frequent scanners in veterinary clinics. To date, these studies in cats do not show a clear distinction of nerve centres in MRI data. Aims: The objective of this study is to determine the efficacy of Mulligan histological staining as a tool in facilitating the location and identification of the main structures of the feline brain in MRI. This study aims to facilitate the interpretation of MRI obtained with these types of scanners. Methods: A total of 10 feline brains were used. One specimen was used for MRI (T2 sequence using a 1.5T scanner). The other 9 brains were sectioned and stained with the three Mulligan staining techniques (Mulligan, Le Masurier and Robert). Results: The uptake of stain by the grey matter in these sections allowed the determination of the location and the limits of these nervous structures within the brain. The histological location of these structures was correlated with the MRI scans, leading to the successful identification of many small, indistinct nuclei. Conclusion: Mulligan staining is proposed as a tool that facilitates the location of nerve structures in comparison with data from the most frequently-used MRI scanners in veterinary clinics.
Open Access
Tipos de fibras en el músculo esquelético del toro de lidia (bos taurus ibericus): estudio histoquímico y morfométrico.
(Murcia: Universidad de Murcia, Servicio de Publicaciones, 1997) Vázquez Autón, José María; Moreno Medina, Francisco; Gil Cano, Francisco; Latorre Reviriego, Rafael Manuel; López Albors, Octavio Miguel; Martínez Gomariz, Francisco; Ramírez Zarzosa, Gregorio José; Facultad de Veterinaria