1989, Moreno Medina, Francisco, Vázquez Autón, José María, Gil Cano, Francisco, Latorre Reviriego, Rafael Manuel, Ramírez Zarzosa, Gregorio José, Facultad de Veterinaria

2021, Diaz Martínez, E., Ayala Florenciano, María Dolores, Arencibia Espinosa, A., Soler Laguía, Marta, Kilroy, D., Martínez Gomariz, Francisco, Ramírez Zarzosa, Gregorio José, Anatomía y Anatomía Patológica Comparada, Facultad de Veterinaria

Background: Despite multiple studies describing accurate diagnoses using advanced neuroimaging techniques, low and mid-field magnetic resonance imaging (MRI) are still the most frequent scanners in veterinary clinics. To date, these studies in cats do not show a clear distinction of nerve centres in MRI data. Aims: The objective of this study is to determine the efficacy of Mulligan histological staining as a tool in facilitating the location and identification of the main structures of the feline brain in MRI. This study aims to facilitate the interpretation of MRI obtained with these types of scanners. Methods: A total of 10 feline brains were used. One specimen was used for MRI (T2 sequence using a 1.5T scanner). The other 9 brains were sectioned and stained with the three Mulligan staining techniques (Mulligan, Le Masurier and Robert). Results: The uptake of stain by the grey matter in these sections allowed the determination of the location and the limits of these nervous structures within the brain. The histological location of these structures was correlated with the MRI scans, leading to the successful identification of many small, indistinct nuclei. Conclusion: Mulligan staining is proposed as a tool that facilitates the location of nerve structures in comparison with data from the most frequently-used MRI scanners in veterinary clinics.

2001, Vázquez, J.Mª, Moreno, F., Gil Cano, Francisco, Latorre Reviriego, Rafael Manuel, López Albors, Octavio Miguel, Ramírez Zarzosa, Gregorio José

Using a variety of histochemical methods -mATPase staining after alkaline and acid preincubations, NADH-TR and a-MGPDH- we have investigated the fibre types in porcine skeletal muscle. The results reveal that four major fibre types -1, IIA, IIB and II*- can be separated histochemically in Longissimus lumborum muscle of Landrace pigs. The histochemical properties of the muscle fibre type 11* are very similar to that of type IIX described in other mammals. The existence of IIX fibres in pig muscle has been recently demonstrated by molecular biology techniques and our results validate the use of histochemistry (mATPase) as an easy methodology to differentiate the three fast myosins (type 11 fibres) in pig muscle.

2021-05-22, García de los Ríos y Loshuertos, Álvaro, Soler Laguía, Marta, Arencibia Espinosa, Alberto, Martínez Gomariz, Francisco, Sánchez Collado, Cayetano, López Fernández, Alfredo, Gil Cano, Francisco, Seva Alcaraz, Juan, Ramírez Zarzosa, Gregorio José, Anatomía y Anatomía Patológica Comparadas

In this work, the fetal and newborn anatomical structures of the dolphin oropharyngeal cavities were studied. The main technique used was endoscopy, as these cavities are narrow tubular spaces and the oral cavity is difficult to photograph without moving the specimen. The endoscope was used to study the mucosal features of the oral and pharyngeal cavities. Two pharyngeal diverticula of the auditory tubes were discovered on either side of the choanae and larynx. These spaces begin close to the musculotubaric channel of the middle ear, are linked to the pterygopalatine recesses (pterygoid sinus) and they extend to the maxillopalatine fossa. Magnetic Resonance Imaging (MRI), osteological analysis, sectional anatomy, dissections, and histology were also used to better understand the function of the pharyngeal diverticula of the auditory tubes. These data were then compared with the horse’s pharyngeal diverticula of the auditory tubes. The histology revealed that a vascular plexus inside these diverticula could help to expel the air from this space to the nasopharynx. In the oral cavity, teeth remain inside the alveolus and covered by gums. The marginal papillae of the tongue differ in extension depending on the fetal specimen studied. The histology reveals that the incisive papilla is vestigial and contain abundant innervation. No ducts were observed inside lateral sublingual folds in the oral cavity proper and caruncles were not seen in the prefrenular space.

2008, Vázquez Autón, José María, Ayala Florenciano, María Dolores, Gil Cano, Francisco, Latorre Reviriego, Rafael Manuel, López Albors, Octavio Miguel, Martínez Gomariz, Francisco, Ramírez Zarzosa, Gregorio José, Sánchez Collado, Cayetano, Facultad de Veterinaria

1997, Vázquez Autón, José María, Moreno Medina, Francisco, Gil Cano, Francisco, Latorre Reviriego, Rafael Manuel, López Albors, Octavio Miguel, Martínez Gomariz, Francisco, Ramírez Zarzosa, Gregorio José, Facultad de Veterinaria

2023-12-14, Rojo Ríos, Daniel, Soler Laguía, Marta, Kilroy, David, Sánchez Collado, Cayetano, García García, María I., Arencibia Espinosa, Alberto, Ayala Florenciano, María Dolores, Gil Cano, Francisco, Martínez Gomariz, Francisco, Ramírez Zarzosa, Gregorio José, Sánchez Collado, Cayetano, Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria

This study describes the anatomical characteristics of the abdominal and pelvic vascular system of two healthy mature female cats via three-dimensional contrast-enhanced computed tomography angiography, non-contrast enhanced magnetic resonance angiography and three-dimensional printing. Volume-rendering computed tomography angiography images were acquired from the ventral aspect using RadiAnt, Amira and OsiriX MD Dicom three-dimensional formats, and three-dimensional printing was obtained and compared with the corresponding computed tomography angiography images. Non-contrast enhanced magnetic resonance angiography was made us ing the time-of-flight imaging in ventral, oblique and lateral views. In addition, three cadavers with colored latex injection were dissected to facilitate the identification of the vascular structures. Three-dimensional computed tomography angiography showed the main vascular structures, whereas with the time-of-flight blood appeared with a high signal intensity compared with associated abdominal and pelvic tissues. Three-dimensional computed tomography angiography images and time-of-flight sequences provided adequate anatomical details of the main arteries and veins that could be used for future feline anatomical and clinical vascular studies of the abdomen and pelvis

2000, Granado, J. D., Chaín Navarro, Celia, Vázquez Autón, José María, Gil Cano, Francisco, Latorre Reviriego, Rafael Manuel, López Albors, Octavio Miguel, Orenes Hernández, Mariano, Ramírez Zarzosa, Gregorio José, Facultad de Veterinaria

2019-12-13, Ríos y Loshuertos, Álvaro García de los, Arencibia Espinosa, Alberto, Soler Laguía, Marta, Gil Cano, Francisco, Martínez Gomariz, Francisco, López Fernández, Alfredo, Ramírez Zarzosa, Gregorio José, Anatomía y Anatomía Patológica Comparada, Facultad de Veterinaria

Our objective was to analyze the main anatomical structures of the dolphin head during its developmental stages. Most dolphin studies use only one fetal specimen due to the difficulty in obtaining these materials. Magnetic resonance imaging (MRI) and computed tomography (CT) of two fetuses (younger and older) and a perinatal specimen cadaver of striped dolphins were scanned. Only the older fetus was frozen and then was transversely cross-sectioned. In addition, gross dissections of the head were made on a perinatal and an adult specimen. In the oral cavity, only the mandible and maxilla teeth have started to erupt, while the most rostral teeth have not yet erupted. No salivary glands and masseter muscle were observed. The melon was well identified in CT/MRI images at early stages of development. CT and MRI images allowed observation of the maxillary sinus. The orbit and eyeball were analyzed and the absence of infraorbital rim together with the temporal process of the zygomatic bone holding periorbit were described. An enlarged auditory tube was identified using anatomical sections, CT, and MRI. We also compare the dolphin head anatomy with some mammals, trying to underline the anatomical and physiological changes and explain them from an ontogenic point of view.

2025-03-12, Díaz Martínez, Elena, Arencibia Espinosa, Alberto, Soler Laguía, Marta, Rojo Ríoz, Daniel, Ortuño Lorente, Ainara, Leotte Sánchez, María, Ceballos Francisco, Diana, García García, María Inmaculada, Kilroy, David, Sánchez Collado, Cayetano, Ayala Florenciano, María Dolores, Gil Cano, Francisco, Martínez Gomariz, Francisco, Ramírez Zarzosa, Gregorio José, Sánchez Collado, Cayetano, Anatomía y Anatomía Patológica Comparadas