Histology and histopathology Vol. 3, nº 3 (1988)

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http://hdl.handle.net/10201/177669

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The influence of matrix components on the morphological differentiation of a proliferating hepatocyte line from liver of newborn mice
(Murcia : F. Hernández, 1987) Lilja, S.; Merker, H. J.; Ghaida, J.
The differentiation behaviour of a liver epithelial cell line of the newborn mouse cultured on various matrix components (Thermanox pure. Thermanox coated with ECM, dried collagen type I and type 11, wet collagen type I and type I11 and on floating collagen) was investigated by electron microscopy. Only during the last few days of pregnancy and up to day 9 p.p. could these cells be isolated using a very delicate method. The cells were smaller than differentiated hepatocytes and proliferated spontaneously. They resembled the socalled oval liver cells. On Thermanox pure or Thermanox coated with ECM, dried collagen type I or type I1 a confluent monolayer developed after about 6 days that consisted of rather flat extended cells which were characterized by short contacts and the absence of any morphological indications of differentiation. On wet collagen the extension area was smaller and the cells were taller. The length of the contact area and the number and size of gap junctions and cell organelles increased. On floating collagen multi-layered aggregates of polygonal cells developed that were characterized by extended cell contacts, bile capillary-like structures and highly developed cell organelles, especially rough endoplastnic reticulum Since differentiation processes can be demonstrated ultrastructurally only on wet collagen, especially on floating collagen, the chemical composition of the sub\trate and a specific matrix-cell interaction cannot be the only triggering factor. It is assumed that mechanical properties of the suhstrate, e.g. plasticity, are involved. The change in the shape of the cell, the prolongation or intensification of the cell contact and the adaptation of the cytoskeleton might play a decisive role in this connection.
Open Access
Metaplasia of the parietal layer of Bowman's capsule in the human kidney. Incidence in alcoholic liver disease and hypertension
(Murcia : F. Hernández, 1988) Haensly, William E.
This report is the second of two surveys to determine the incidence of metaplasia of Bowman's parietal epithelium in the human kidney. Human kidney sections obtained at autopsy at the Department of Pathology, University of Texas Medical Branch, Galveston, Texas, were examined with the light microscope. The kidneys were fixed in neutral formalin, sectioned at 6 pm and stained with hematoxylin and eosin. Autopsy records were consulted after kidney section examination to determine if there was any correlation between clinical disease, histopathological changes in organ systems and metaplasia of Bowman's capsule. The kidney sections represented both sexes in 8 age groups, from less than one year to 80 years. A total of 174 kidneys, representing 174 individuals, were evaluated. One hundred renal corpuscles were counted per section and the parietal layer of Bowman's capsule was classified as normal (squamous) or metaplastic (cuboidal). Of the 174 kidneys examined, 137 (79% )- 79 male and 58 female - had metaplasia of Bowman's capsule. On the average, in the kidneys with the lesion, 6% of the renal corpuscles had metaplasia of Bowman's parietal layer. The lesion was present in both sexes in all age groups. The autopsy records revealed that metaplasia of Bowman's parietal epithelium was usually present with hepatic fatty changes andlor congestion. Alcoholic liver disease and hypertension represented the most frequent clinical diseases in the sample; these conditions had the highest incidence of metaplasia. Twenty-six of the 174 kidney samples were from individuals with alcoholic liver disease, all of whom had metaplasia of Bowman's capsule. In the latter kidneys the mean percentage of glomerular capsules with metaplasia was 14%, with a range of l to 46%. Thirtyeight of the 174 kidney samples were from individuals with hypertension, 29 (76% ) of whom had metaplasia of Bowman's capsule. In these kidneys the mean percentage of glomerular capsules with metaplasia was 6% with a range of 1 to 16%. These observations suggest that metaplasia of Bowman's parietal epithelium is a common occurrence in the human kidney under different pathological conditions, and is especially prominent in alcoholic liver disease and hypertension.
Open Access
Pituitary growth hormone secretory granules in streptozotocin-induced diabetic rats
(Murcia : F. Hernández, 1988) Masataka Shiino; Toshiko Ishibashi
The ultrastructural features of growth hormone (GH) secretory granules were observed by immuno-gold procedure in the anterior pituitary glands of streptozotocin (STZ) - induced diabetic rats. The diabetic state of rats induced by one injection of STZ was severely advanced at 10 months after the injection. The cytoplasmic organella were revealed to be poorly developed in GH-cells of the diabetic rats. The size of immunoreactive GH-granules of 10-months-old diabetic rats was smaller than that of sham-controls. The insulin therapy in the diabetic rats resulted in a considerable improvement in relation to the changes seen in the GHcells, but the therapy did not return the profile of the granule size distribution to that of the control group
Open Access
Eosin-related fluorescence of acidophil pituitary cells
(Murcia : F. Hernández, 1988) Friedman, H.; Friedman, I.V.C.; Mello, C.V.
The examination of haematoxylin and eosin stained sections of normal and neoplastic pituitary glands under ultraviolet light illumination discloses fluorescence of acidophil cells. The distinction between prolactin and growth hormone-producing cells is not possible. Such fluorescence depends on previous eosin staining.
Open Access
The fibrogenic response of adult rat lung to continuous propranolol treatment
(Murcia : F. Hernández, 1988) Smith, Dennis M.; Sommers Smith, Sally K.
Fibrogenesis is a common pulmonary response to injury, which is usually preceded by other severe reactions, including inflammation, fluid exudation, and alveolar epithelial damage and proliferation. The purpose of this study was to examine the morphologic effects on the distal lung of a continuous propranolol treatment. Adult male rats were treated, via a subcutaneous osmotic pump, with a continuous (approximately 0.5 mglhour) dose of propranolol HCl, a potent wide range beta-adrenergic blocking agent, in saline, or saline alone. The animals were killed after one week or three weeks. Electron microscopy of the lungs of the propranolol-treated animals revealed a dramatic increase in the prominence of interstitial cells and fibers of the alveolar septa, along with focal thickening of endothelial cells and some morphologic changes in type I1 alveolar epithelial cells. In some animals an analysis of total protein content, as well as 3H-proline incorporation into total protein and collagen was undertaken. The results of this study indicated a significant increase in total protein content and proline incorporation into collagen in the lungs of animals treated for seven days with continuous propranolol. There was no evidence of stimulated blood cells, macrophages, edema or severe epithelial damage. This study provides morphologic evidence that continuous treatment with moderate levels of propranolol results in a fibrogenic response in the peripheral lung, in the absence of typical hallmarks of severe pulmonary damage.

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