Histology and histopathology Vol.34, nº5 (2019)
Ir a Estadísticas
Permanent URI for this collection
Browse
Recent Submissions
- PublicationOpen AccessInfiltrated M2 tumour-associated macrophages in the stroma promote metastasis and poor survival in oesophageal squamous cell carcinoma(2019) Zhou, Jian; Zheng, Shutao; Liu, Tao; Liu, Qing; Chen, Yumei; Ma, Rong; Tan, Doudou
- PublicationOpen AccessEvaluation of retinal injury in a rat model of transient ischemic stroke(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Wang, Saibin; Ye, Qian; Tu, JunweiStroke-associated ocular disorders are visionthreatening. This study was designed to evaluate in vivo retinal injury induced by transient global cerebral ischemia/reperfusion (I/R). A stroke-induced retinal injury model in Wistar rats was established by electrocoagulation of bilateral vertebral arteries, combined with transient ligation of the bilateral common carotid arteries. Rats were randomly divided into groups based on the time post cerebral perfusion (3 h, 24 h, 48 h, 72 h, and 7 days). Retinal injury was evaluated by histological analysis, examination of eye fundus, and TUNEL staining. The expression of protein kinase Calpha (PKCα) and fibrillary acidic protein (GFAP) was determined using qRT-PCR and immunofluorescence analysis. Both retinal neurons and the vasculature underwent significant damage in the cerebral-I/R groups when compared to rats in the sham group. Moreover, when compared to non-stroke rats, TUNEL staining revealed signs of apoptosis in the retina after transient ischemic stroke was induced (P<0.001). In these rats, the expression of PKCα and GFAP in the retinas was enhanced and peaked at 72 h after induction of cerebralI/R (P<0.001). In this study, we found that retinas are very susceptible to transient global cerebral-I/R injury. The expression of PKCα and GFAP may be implicated in the pathogenesis of ischemic stroke-induced retinal injury.
- PublicationOpen AccessA plant proteinase inhibitor from Enterolobium contortisiliquum attenuates airway hyperresponsiveness, inflammation and remodeling in a mouse model of asthma(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Dias Rodrigues, Adriana Palmeira; Santos Bortolozzo, Anelize Sartori; Arantes Costa, Fernanda Magalhães; Saraiva Romanholo, Beatriz Mangueira; Castro Ribas de Souza, Flávia; Brüggemann, Thayse Regina; Roncon Santana, Fernanda Paula; Vilela de Brito, Marlon; Ramalho Bonturi, Camila; Neto dos Santos Nunes, Natalia; Máximo Prado, Carla; Leick, Edna Aparecida; Vilela Oliva, Maria Luiza; de Arruda Martins, Milton; Fraga Righetti, Renato; Lopes Calvo Tibério, Iolanda de FátimaIntroduction. Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for asthma. Purpose. The aim of the present study was to evaluate the effects of Enterolobium contortisiliquum trypsin inhibitor (EcTI) on pulmonary mechanical function, eosinophilic recruitment, inflammatory cytokines, remodeling and oxidative stress in an experimental model of chronic allergic pulmonary inflammation. Methods. BALB/c mice were divided into 4 groups: C (saline i.p and inhalations with saline), OVA (ovalbumin i.p and inhalations with ovalbumin); C+EC (saline i.p, inhalations with s aline and treatment with EcTI); OVA+EC (ovalbumin i.p, inhalations with ovalbumin and treatment with EcTI). On day 29, we performed the following tests: resistance (Rrs) and elastance (Ers) of the respiratory system; (b) quantify eosinophils, 8-ISO-PGF2α, collagen and elastic fiber volume fractions; (c) IFN-γ, IL-4, IL-5, IL-13, MMP-9, TIMP-1, TGF-β, iNOS and p65-NFκB-positive cells in the airway and alveolar walls. Results. In OVA+EC group, there was an attenuation of the Rrs and Ers, reduction of eosinophils, IL-4, IL-5, IL-13, IFN-γ, iNOS and p65-NF κB-positive cells compared to OVA group. The 8-ISO-PGF2α, elastic and collagen fibers volume fractions as well as the positive cells for MMP-9, TIMP1 and TGF-β positive cells were decreased in OVA+EC compared to the OVA group. Conclusion. EcTI attenuates bronchial hyperresponsiveness, inflammation, remodeling and oxidative stress activation in this experimental mouse model of asthma.
- PublicationOpen AccessImpaired spermatogenesis, tubular wall disruption, altered blood-testis barrier composition and intratubular lymphocytes in an infertile Beagle dog – a putative case of autoimmune orchitis(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Matschurat, Carolin; Rode, Kristina; Hollenbach, Julia; Wolf, Karola; Urhausen, Carola; Beineke, Andreas; Günzel Apel, Anne Rose; Brehm, RalphImpairment of blood-testis barrier integrity can be observed during inflammation, infection, trauma and experimental autoimmune orchitis, which is inducible in rodents. In the present study, an initially fertile two-year-old Beagle dog was presented with a decline in total sperm number resulting in azoospermia within five months, verified by twice-monthly semen analyses. The dog was clinically healthy with bilateral small testes and showed normal thyroid function. Bacterial cultures of semen were negative and serum biochemical analyses showed no abnormal findings. To determine causes of azoospermia, the dog was castrated. Histological examinations of hematoxylin-eosin stained testicular sections revealed impaired spermatogenesis, seminiferous tubules with spermatogenic arrest or Sertoli-cell-only syndrome as well as focal interstitial and even intratubular lymphocytic infiltrations. Germ cell sloughing, apoptosis and giant cells were also observed in some tubules. Subsequent immunostainings of smooth-muscle-actin, claudin3, claudin11 and connexin43 demonstrated, for the first time, a mechanical and functional disruption of the tubular wall and alterations of blood-testis barrier proteins in these tubules. Presence of claudin3 and claudin11 in canine testis was confirmed using RT-PCR and sequencing and/ or Western-blot analyses. All findings suggested a possible spontaneous autoimmune orchitis to be the underlying cause for the observed azoospermia.
- PublicationOpen AccessUmbilical cord-derived mesenchymal stromal/stem cells enhance recovery of surgically induced skeletal muscle ischemia in a rat model(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Arutyunyan, Irina; Fatkhudinov, Timur; Elchaninov, Andrey; Vasyukova, Olesya; Makarov, Andrey; Usman, Natalia; Kananykhina, Evgeniya; Lokhonina, Anastasiya; Goldshtein, Dmitry; Bolshakova, Galina; Sukhikh, GennadyThis study delves into possible mechanisms underlying the stimulating influence of UC-MSCs transplantation on functional and structural recovery of ischemic skeletal muscles. Limb ischemia was created in Sprague-Dawley rats by excision of femoral and popliteal arteries. Allogeneic rat PKH26-labeled UCMSCs were administered by direct intramuscular injection. Animals of experimental group responded to the transplantation by improvement in their locomotor function as assessed by the rotarod performance test on day 9 and 29 after transplantation. Histomorphometric analysis showed that relative area of the lesions in the experimental group was significantly smaller than in the control group at all time points during the observation. Calculated densities of microcirculation vessels within the lesions were significantly higher in the experimental group than in the control group on day 10 after transplantation. Only a part of the transplanted allogeneic UC-MSCs survived within the ischemic muscle tissue, and a considerable portion of these surviving cells were found alongside the VEGFproducing preserved muscle fibers. The РКН26 label was not found within the walls of capillaries or larger blood vessels. The administration of allogeneic UCMSCs significantly increased the proportion of M2 macrophages, exhibiting proangiogenic and antiinflammatory properties, for at least 10 days following the transplantation
- «
- 1 (current)
- 2
- 3
- »