Publication: Evaluation of retinal injury in a rat model of transient ischemic stroke
Authors
Wang, Saibin ; Ye, Qian ; Tu, Junwei
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-18-060
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info:eu-repo/semantics/article
Description
Abstract
Stroke-associated ocular disorders are visionthreatening. This study was designed to evaluate in vivo
retinal injury induced by transient global cerebral
ischemia/reperfusion (I/R). A stroke-induced retinal
injury model in Wistar rats was established by
electrocoagulation of bilateral vertebral arteries,
combined with transient ligation of the bilateral common
carotid arteries. Rats were randomly divided into groups
based on the time post cerebral perfusion (3 h, 24 h, 48
h, 72 h, and 7 days). Retinal injury was evaluated by
histological analysis, examination of eye fundus, and
TUNEL staining. The expression of protein kinase Calpha (PKCα) and fibrillary acidic protein (GFAP) was
determined using qRT-PCR and immunofluorescence
analysis. Both retinal neurons and the vasculature
underwent significant damage in the cerebral-I/R groups
when compared to rats in the sham group. Moreover,
when compared to non-stroke rats, TUNEL staining
revealed signs of apoptosis in the retina after transient
ischemic stroke was induced (P<0.001). In these rats, the
expression of PKCα and GFAP in the retinas was
enhanced and peaked at 72 h after induction of cerebralI/R (P<0.001). In this study, we found that retinas are
very susceptible to transient global cerebral-I/R injury.
The expression of PKCα and GFAP may be implicated
in the pathogenesis of ischemic stroke-induced retinal
injury.
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Citation
Histology and Histopathology, Vol.34, nº5, (2019)
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