Histology and histopathology Vol.13, nº 2 (1998)
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- PublicationOpen AccessLow-intensity ultrasound energy applied to the testes of aged rats(Murcia : F. Hernández, 1998) Haddad, S.; Franci, J.A.A.; Petenusci, S.O.; Lamano Carvalho, T.L.Previous studies from our laboratory have shown that low-intensity ultrasound applied to the scrotum of prepubertal rats causes a 62% increase in plasma testosterone, suggesting a possible stimulation of LH receptors andtor the enzymes controlling the steroidogenic process. The purpose of the present study was to investigate whether low-intensity ultrasound has a stimulatory effect on the androgenic activity of aged testes. In addition to plasma testosterone, LH and FSH, the testicular spermatogenic status was also analysed. Ultrasound applied to the scrotum of aged rats did not stimulate sperm production, which was significantly reduced compared to sexually mature animals, and failed to re-establish the steroidogenic testicular function, which was decreased by 74%, suggesting an inherent loss of gonadal steroidogenic competence.
- PublicationOpen AccessThe torus longitudinalis in the gilthead seabream, an undescribed fiber tract link with the valvula cerebelli(Murcia : F. Hernández, 1998) Muñoz-Cueto, J.A.; Sarasquete, C.; Kah, O.In this paper, we present a brief anatomical description of the torus longitudinalis and the valvula cerebelli in a percomorph fish, the gilthead seabream (Sparus aurata) based on the analysis of serial brain sections stained by paraldehyde fuchsin, Groat's hematoxylin-picroindigocarmin and cresyl violet. The existence of a small undescribed fiber tract directly bridging the ventral torus longitudinalis and the granular layer of the rostral valvula cerebelli is also reported. This small fiber tract was observed in its integrity on a few transverse sections providing that the angle of sectioning was appropiate. The existence of the anatomical link between the TL and the VC described in this paper might sustain the role of the TL as part of an ascending cerebello-tectal circuit, at least in gilthead seabream. However, these fibers might also represent fibers of passage originating from other brain structures.
- PublicationOpen AccessSynaptophysin immunoreactivity in the cat cochlear nuclei(Murcia : F. Hernández, 1998) Gil-Loyzaga, P.; Bartolome, M.V.; Ibanez, A.The presence of synaptophysin, a presynaptic ca2+ binding glycoprotein, has been analyzed in the cochlear nucleus complex of the adult cat using an antisynaptophysin monoclonal antibody. Synaptophysin immunoreactivity was differently distributed between regions of cochlear nuclei. Terminal boutons contacting directly with neurons (cell bodies and dendrites) or in the neuropil of ventral and dorsal cochlear nuclei appeared immunostained. In the ventral cochlear nucleus, synaptophysin-labelled boutons were clearly defined in contact with spherical, globular, multipolar, octopus and cochlear-nerve root neurons. However, the dorsal cochlear nucleus showed a dense immunostained network of synaptophysin-labelled puncta, inside which some neuron cell bodies were observed. The present findings show a differential distribution of synaptophysin- immunostained boutons between the ventral and the dorsal cochlear nuclei of the cat. The present description of the different synaptophysin-labelled terminal boutons in the cochlear nuclei complex and their pattern of distribution, will be useful for further studies on development, degeneration or regeneration of the peripheral auditory pathway.
- PublicationOpen AccessThe induction of gut hyperplasia by phytohaemagglutinin in the diet and limitation of tumour growth(Murcia : F. Hernández, 1998) Pryme, I.F.; Pusztai, A.; Bardocz, S.; Ewen, S.W.B.The growth of a transplantable murine non- Hodgkin lymphoma tumour, developing either intraperitoneally as an ascites tumour or subcutaneously as a solid tumour, has been shown to be markedly diminished by including phytohaemagglutinin (PHA), a lectin present in raw kidney bean (I'haseolus vulgaris) in the diet. In NMRl mice fed PHA within the range 0.45-7.0 mg/g diet, tumours which developed during a 10 day period after subcutaneous injection of cells were about 35% of the dry weight of those in lactalbumin-fed (control) animals. The reduced rate of growth occurred in a dose-dependent manner within the range 0.45-3.5 mg/g diet. Based on these observations it has been suggested that a competition between the gut epithelium undergoing hyperplasia and the developing tumour may occur for nutrients from a common body pool, and this may be an important factor with regard to the observed initial low level of tumour growth following the feeding of a PHA-containing diet. Observations which showed that the level of hyperplasia of the small bowel in response to feeding the PHA diets was higher in noninjected mice compared to those which had been injected with tumour cells substantiated the concept of competition between gut and tumour for nutrients etc. required for growth. Experiments with a second murine tumour cell line (a plasmacytoma) in Balblc mice gave similar results indicating that the effect of PHA was not restricted to a single tumour system.
- PublicationOpen AccessDistribution of VIP receptors in the human submandibular gland, an immunohistochemical study(Murcia : F. Hernández, 1998) Kusakabe, T.; Matsuda, H.; Gono, Y.; Kawakami, T.; Kurihara, K.; Tsukuda, M.; Takenaka, T.Distribution of vasoactive intestinal polypeptide (VIP)-immunoreactive nerve fibers and VIPreceptor (VIP-R)-immunoreactive sites in the human submandibular gland were examined by the peroxidaseantiperoxidase method using the specimens taken from patients that had not received radiotherapy. VIP-immunoreactive fibers were found around both serous and mucous acini, the duct system, and those around the mucous acini were more numerous than those around the serous acini. VIP-R immunoreactivity was restricted to the mucous acini and the intercalated duct segment. The serous acini, striated duct, and excretory ducts lacked VIP-R immunoreactivity. These findings suggest that the mucous acinar cells contain VIP-R, and that VIP-R-mediated VIP action is involved in regulating synthesis of viscous saliva and its release.