Browsing by Subject "Wound"

Now showing 1 - 7 of 7
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    Antibacterial effect of Spanish honeys of different botanical origins against Staphylococcus epidermidis
    (MDPI, 2024-06-15) Núñez Gómez, Vanesa; San Mateo Molina, Marta; Sánchez Martínez, Lorena; Periago Castón, María Jesús; Tecnología de Alimentos, Nutrición y Bromatología; Facultad de Veterinaria
    Honey is traditionally used for its medicinal properties attributed to its antibacterial and antioxidant effects. It is considered a natural alternative to conventional antibiotics. This effect has been attributed to their physico-chemical properties, as various chemical parameters can synergistically influence this effect. The aim of this study is to assess Spanish honeys of diverse botanical origins for their antibacterial efficacy against Staphylococcus epidermidis, correlating their physico-chemical attributes, (poly)phenol content, and antioxidant activity. The methods included colour determination via two methodologies, acidity, pH, moisture content, and sugar concentration. (Poly)phenol content was quantified using the Folin-Ciocalteau method, while antioxidant activity was evaluated via the FRAP method. Subsequently, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against S. epidermidis were investigated with different concentrations of honeys. The results revealed a direct relationship between honey darkness, (poly)phenol concentration, antioxidant activity, and antibacterial efficacy. Darker honeys exhibited higher (poly)phenol levels, greater antioxidant activity, and consequently, lower MIC and MBC values, showing enhanced antibacterial properties. These findings underscore the potential of honey as a therapeutic agent against S. epidermidis, particularly in wound healing applications to avoid infection. Further research into honey’s multifaceted properties is warranted to unveil novel therapeutic avenues in healthcare
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    Calycosin accelerates wound healing in diabetic rats by alleviating oxidative stress and promoting angiogenesis
    (2026) Min Lu; Lifen Xue; Mi Zhou; Meifeng Zhang; Huifeng Zhu; Wei Lu; Biología Celular e Histología; Universidad de Murcia, Departamento de Biología Celular e Histología
    Background. Angiogenesis is a physiological process of diabetic wound healing. Although calycosin has been reported to exert protective effects on diabetic nephropathy, its role and mechanisms in diabetic wound healing remain unclear. This study investigates the effects of calycosin on wound healing and angiogenesis, and the role of the Nrf2/HO-1 pathway in mitigating oxidative stress in diabetic rats. Methods. In vivo, type 2 diabetes (T2DM) in Sprague-Dawley (SD) rats was induced by a high-fat diet for six weeks combined with a single intraperitoneal injection of 45 mg/kg streptozotocin (STZ). The anesthetized diabetic rats underwent a full skin excision on the back and were then treated with calycosin for two weeks to evaluate the protective effect of calycosin on oxidative stress associated with the Nrf2/HO-1 pathway in diabetic wound rats. In vitro, damage to Human Umbilical Vein Vascular Endothelial Cells (HUVECs) was induced by high glucose, and then treated with calycosin or combined with Nrf2 agonist to evaluate whether calycosin affects cell activity and inhibits oxidative damage via the Nrf2/HO-1 pathway. Results. Our results indicate that calycosin promotes angiogenesis by activating the Nrf2/HO-1 signaling pathway and upregulating downstream antioxidant genes, thereby accelerating wound healing. In vitro studies have also shown that Nrf2/HO-1 signaling activation can enhance the promoting effect of calycosin on cell activity and the inhibitory effect on oxidative stress in HUVECs induced by high glucose. Conclusion. Our results show that calycosin can accelerate wound healing by promoting angiogenesis and inhibiting oxidative stress mediated by the Nrf2/HO 1 pathway, which provides a theoretical basis for the treatment of refractory diabetic wounds.
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    Evaluación de la calidad del cierre de pared abdominal en modelos murinos realizada por alumnos de grado en Medicina.
    (Universidad de Murcia. Servicio de publicaciones, 2022) Gonzalez Lee Chong, Fatima M; Rodriguez Paz, Carlos Agustín; Moreno Ruiz, Alexia; Sánchez de Alba, Luis G; Ramírez Ramos, Kevin
    Resumen: Introducción: Se debe mejorar la docencia quirúrgica, siempre en beneficio del paciente,para que no curse con efectos adversos. Objetivo: Evaluar la calidad del cierre de pared abdominal en modelos murinos realizada por alumnos del grado en medicina. Métodos: Estudio observacional analítico, prospectivo y transversal. Se consideró conjunto universo a 55 alumnos delos cuales solo se incluyen a 35 seleccionados, a los que se les evalúo sobre modelos murinos tiporatas Wistar, tomando como variables el cierre de pared abdominal y las calificaciones según elcuestionario Anaya y Serrano. Resultados: Se evaluaron 35 cierres de pared abdominal, 23 de ellos con calidad (65.7 %) y 12, que no fueron de calidad (34.3 %). Se observa una proporción de cierres de calidad en alumnos aprobados (f=18, 78.3%) frente a no aprobados (f= 5, 21.7%) y también en los cierres de no calidad en alumnos aprobados (f=8, 66.7%) frente a no aprobados (f=4, 33.3%).Conclusión: Los cierres de pared abdominal no exitosos en modelos murinos no se relacionan con las calificaciones reprobatorias.
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    Physical therapy in burn wound healing: Development of clinical prediction rules to identify the efficacy of pulsed electromagnetic therapy
    (Universidad de Murcia, Servicio de publicaciones, 2024) Nagy, Ahmed Mohamed; Elsayeh, Shaimaa Mohamed Ahmed; Bayoumi, Mohamed Bayoumi Ibrahim; Metawee, Shimaa Mohamed; Ahmed, Omnia Saeed Mahmoud; Saafan, Karim Ibrahim
    Many studies have demonstrated the effect of pulsed electromagnetic therapy (PEMT) on wound healing. This study aimed to develop a clinical prediction rule (CPR) to assess PEMT's efficacy in burn healing, potentially enhancing treatment decisions and outcomes. It was a one-group intervention study with 46 patients (21 males, 25 females) aged 20 to 55 years, having partial-thickness burns in the first or second healing stage, and a total burned surface area (TBSA) over 15%. The intervention involved pulsed electromagnetic therapy (Fisioline, Italy) for up to six weeks, with 60-minute sessions at 12 Hz and 12 Gauss, three times per week. The statistical analysis was conducted using the SPSS. The study revealed a significant decline in wound surface area (WSA) post-intervention (p < 0.05). A significant negative relationship was found between wound improvement and both age and total body surface area (TBSA) (p < 0.05), while a significant positive relationship was observed between wound improvement and initial wound size (p < 0.05). No significant relationship was found between wound improvement and wound stage (p > 0.05). The study concluded that pulsed electromagnetic therapy significantly reduces wound surface area. Additionally, age, TBSA, and initial burn wound size are important predictors of the therapy's efficacy in treating burn wounds.
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    La representación de la cicatriz en la poesía de Luna Miguel
    (Universidad de Murcia, Editum, 2019) Úbeda Sánchez, Patricia
    En el presente artículo se va a analizar la poesía de Luna Miguel a partir de la herida y la cicatriz, dos núcleos significativos de la poesía de esta poeta. Estos dos motivos e structuran el imaginario de la poeta. La importancia del cuerpo se enlaza con la búsqueda de la individualidad y la nostalgia de la infancia perdida. El cuerpo es el territorio en el que la voz poética inscribe la violencia, los impulsos más íntimos. La línea metodológica es interdisciplinar, en la que el cuerpo va a ser el objeto de estudio y cómo interviene en la escritura. El objetivo de este artículo es mostrar la escritura corpórea, teorizada por Hélène Cixous, se inscribe en la poesía migueliana mediante la herida y sus procesos de cicatricación con la finalidad de descubrir los mecanismos corpóreos de la escritura poética
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    Role of cytokines in host defense against Staphylococcus aureus skin infection
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Wu, Guosheng; Zhu, Banghui; Hong, Xudong; Luo, Pengfei; Xia, Zhaofan
    Wound infection caused by Staphylococcus aureus (S. aureus) is a critical clinical problem due to long hospitalization times, significant morbidity and mortality, as well as considerable medical resource consumption. With the emergence of methicillinresistant S. aureus (MRSA) strains, current antibiotic treatments are becoming ineffective in combatting S. aureus infection. Thus, a novel therapeutic strategy is required. Recent studies discovered that several cytokines in the infected wound area play protective roles against S. aureus infection. This review summarizes recent discoveries regarding the role of cytokines-mediated responses in host defense against S. aureus skin infection, and discusses their implications for future immunotherapy and vaccine development.
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    Skin mucus metabolomics provides insights into the interplay between diet and wound in gilthead seabream (Sparus aurata)
    (Frontiers, ) Albaladejo-Riad, Nora; Espinosa-Ruiz, Cristóbal; Lazado, Carlos C.; Esteban Abad, María de los Ángeles; Biología Celular e Histología
    Ultra Performance Liquid Chromatography coupled with a high-resolution quadrupole-orbitrap mass spectrometry DATA