Browsing by Subject "Regulatory peptides"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    Open Access
    Immunolocalization of regulatory peptides and 5-HT in bovine male urogenital apparatus
    (Murcia : F. Hernández, 1998) Arrighi, S.; Domeneghini, C.
    Specimens of testis, excurrent duct including the accessory genital glands and urethra throughout its extension were investigated in adult bovines, in order to imm~~nohistochemicalllyo calize both the peptidergic innervation and the epithelial cell types belonging to [he diffuse endocrine system (DES). Immunoreactivities to GRP, met- and leu-enkephalins, CGRP, NPY, substance P, VIP, somatostatin, l3-endorphin and 5-HT antisera were tested by means of a labelled streptavidin-biotin (LSAB) method. Such regulatory substances were found in components of the peripheral nervous system (nerve fibers in the connective and muscular tissues, sub- and intraepithelial nerve terminals, nerve cell bodies and fibers in intramural ganglia), and in epithelia1 endocrine/ paracrine cells. Bovine urogenital apparatus is supplied by many peptide-containing nerves, which contain in many localizations GRP and enkephalins, and to a lesser extent substance P, CGRP, NPY and VIP. A thin network of peptidergic nerves distributes to the musculature of the canalicular organs and accessory glands. The prostatic complex was especially rich in peptidergic innervation, and also contained somatostatin- and 5-HTsecreting endocrine cells. In addition, 5-HT-immunoreactive endocrine cells were found in the bulbourethral gland and urethral epithelium. CGRP-ir nerves were present contacting striated muscle fibers of urethra (motor end plates). The testis was devoid of any immunoreactivity. These data are compared with those obtained in a companion study carried out the same organs in two species of Equidae (Equus caballus and Equt~sa sinus). Different patterns of immunoreactivities can be outlined in these domestic ungulates.
  • Thumbnail Image
    Publication
    Open Access
    Localization of regulatory peptides in the male urogenital apparatus of domestic equidae: a comparative immunohistochemical study in Equus caballus and Equus asinus
    (Murcia : F. Hernández, 1997) Arrighi, S.; Domeneghini, C.
    An immunohistochemical study was carried out on specimens of testis, excurrent duct including the male accessory glands and urethra in its various tracts in the horse and the donkey, in order to localize nine regulatory peptides. Immunoreactivities were tested by means of Labelled Strept Avidin-Biotin (LSAB) method. The study has shown that Equine male genitalia are supplied by many peptide immunoreactive nerves containing NPY-, VIP-, leu- and met-Enkephalin-, Substance P-, CGRP- and BombesinIGRP-like peptides, each of them having a characteristic distribution pattern. These neurotransmitters were localized in nerve fibers running in the connective tissue or in contact with the smooth muscle cells, as well as in sub- and intraepithelial nerve terminals, and in perivascular nerve fibers. In addition, leu- and met-Enkephalin-likeimmunoreactive endocrine cells were shown in the bulbourethral gland of the horse. In both species it was evident that an extensive utilization of NPY and VIP exists. A contingent of NPY- and VIP-ir nerve fibers may have an intrinsic origin. The other regulatory peptides tested show a characteristic distribution pattern, limited to some organs and peculiar to each of the two species of Equidae. Differences observed comparing E. caballus and E. asinus might be related to the speciesspecific balance of the accessory neurotransmitters which in turn accompany adrenergic innervation. In both species it is noteworthy the complete absence of any regulatory peptide in the testis, with the exception of the perivascular localization of NPY-ir neme fibers.