Publication: Immunolocalization of regulatory peptides and 5-HT in bovine male urogenital apparatus
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Date
1998
Authors
Arrighi, S. ; Domeneghini, C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Specimens of testis, excurrent duct including
the accessory genital glands and urethra throughout its
extension were investigated in adult bovines, in order to
imm~~nohistochemicalllyo calize both the peptidergic
innervation and the epithelial cell types belonging to [he
diffuse endocrine system (DES). Immunoreactivities to
GRP, met- and leu-enkephalins, CGRP, NPY, substance
P, VIP, somatostatin, l3-endorphin and 5-HT antisera
were tested by means of a labelled streptavidin-biotin
(LSAB) method. Such regulatory substances were found
in components of the peripheral nervous system (nerve
fibers in the connective and muscular tissues, sub- and
intraepithelial nerve terminals, nerve cell bodies and
fibers in intramural ganglia), and in epithelia1 endocrine/
paracrine cells. Bovine urogenital apparatus is supplied
by many peptide-containing nerves, which contain in
many localizations GRP and enkephalins, and to a lesser
extent substance P, CGRP, NPY and VIP. A thin network
of peptidergic nerves distributes to the musculature of
the canalicular organs and accessory glands. The
prostatic complex was especially rich in peptidergic
innervation, and also contained somatostatin- and 5-HTsecreting
endocrine cells. In addition, 5-HT-immunoreactive
endocrine cells were found in the bulbourethral
gland and urethral epithelium. CGRP-ir nerves were
present contacting striated muscle fibers of urethra
(motor end plates). The testis was devoid of any
immunoreactivity. These data are compared with those
obtained in a companion study carried out the same
organs in two species of Equidae (Equus caballus and
Equt~sa sinus). Different patterns of immunoreactivities
can be outlined in these domestic ungulates.
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