Browsing by Subject "Rat model"

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    Effects of topical applications of porcine acellular urinary bladder matrix and Centella asiatica extract on oral wound healing in a rat model
    (Springer, 2019) Camacho Alonso, Fabio; Torralba Ruiz, M. R.; García Carrillo, N.; Lacal Luján, J.; Martínez Díaz, F.; Sánchez Siles, M.; Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
    Objectives To evaluate the effects of topical applications of porcine acellular urinary bladder matrix (AUBM) and Centella asiatica extract (CAE) on the healing of tongue wounds in a rat model. Materials and methods Wounds were made in the tongue using a punch tool in 64 male Sprague-Dawley rats, randomized into four groups (n = 16 per group): group 1 (control), group 2 (CAE), group 3 (AUBM mixed with orabase), and group 4 (orabase). No product was applied in group 1 and groups 2–4 received three daily topical applications. The animals were weighed on day 0 and at the time of euthanasia. Four rats in each group were euthanized at days 2, 7, 14, and 21 and the tongues were processed for: macroscopic morphometric analysis, myeloperoxidase (MPO) and malondialdehyde (MDA) levels, histological wound repair (degree of reepithelialization and inflammation), and CD31 positivity. Results The animals’ weight gain, histological wound repair, and CD31 positivity from greatest to least were: AUBM > CAE > orabase > control. Percentage of tongue occupied by wound, MPO, and MPA levels from least to greatest were: AUBM < CAE < orabase < control, whereby the AUBM group showed significant differences (p ≤ 0.05) in comparison with the other groups on days 2, 7, 14, and 21 for percentage of tongue occupied by wound and MDA and on days 7, 14, and 21 for MPO. Conclusions CAE is effective for oral tissue regeneration, while AUBM is an even more potent means of oral mucosa regeneration. Clinical relevance AUBM may be beneficial to patients with oral wounds; this finding requires further clinical and laboratory investigation.
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    Effects of topical applications of porcine acellular urinary bladder matrix and Centella asiatica extract on oral wound healing in a rat model
    (Springer Heidelberg, 2018-09-24) Camacho Alonso, Fabio; Torralba Ruiz, M.R.; García Carrillo, N.; Lacal Luján, J.; Martínez Díaz, F.; Sánchez Siles, M.; Dermatología, Estomatología, Radiología y Medicina Física
    Objectives To evaluate the effects of topical applications of porcine acellular urinary bladder matrix (AUBM) and Centella asiatica extract (CAE) on the healing of tongue wounds in a rat model. Materials and methods Wounds were made in the tongue using a punch tool in 64 male Sprague-Dawley rats, randomized into four groups (n = 16 per group): group 1 (control), group 2 (CAE), group 3 (AUBM mixed with orabase), and group 4 (orabase). No product was applied in group 1 and groups 2–4 received three daily topical applications. The animals were weighed on day 0 and at the time of euthanasia. Four rats in each group were euthanized at days 2, 7, 14, and 21 and the tongues were processed for: macroscopic morphometric analysis, myeloperoxidase (MPO) and malondialdehyde (MDA) levels, histological wound repair (degree of reepithelialization and inflammation), and CD31 positivity. Results The animals’ weight gain, histological wound repair, and CD31 positivity from greatest to least were: AUBM > CAE > orabase > control. Percentage of tongue occupied by wound, MPO, and MPA levels from least to greatest were: AUBM < CAE < orabase < control, whereby the AUBM group showed significant differences (p ≤ 0.05) in comparison with the other groups on days 2, 7, 14, and 21 for percentage of tongue occupied by wound and MDA and on days 7, 14, and 21 for MPO. Conclusions CAE is effective for oral tissue regeneration, while AUBM is an even more potent means of oral mucosa regeneration. Clinical relevance AUBM may be beneficial to patients with oral wounds; this finding requires further clinical and laboratory investigation.
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    Genistein attenuates monocrotaline-induced pulmonary arterial hypertension in rats by activating PI3K/Akt/eNOS signaling
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Zheng, Zeqi; Yu, Songping; Zhang, Wan; Peng, Yongchao; Pu, Mingyu; Kang, Ting; Zeng, Junyi; Yu, Yuefei; Li, Guorong
    Introduction: Phytoestrogen genistein may be useful to treat pulmonary arterial hypertension (PAH). However, its mechanism is still not clear. The aim of the present study was to confirm the therapeutic effects of phytoestrogen genistein on PAH in monocrotalineinduced rat model and to explore its mechanism. Materials and Methods: Sprague-Dawley male rats were randomly divided into 4 groups: control group (n=8), PAH group (n=8), genistein treament group with three different doses (n=8 in each dose group) and group of PI3K inhibitor LY294002. The rat model of PAH was induced by monocrotaline (MCT). The situation of survival of rats was observed. Pathological studies of lung and heart tissues were performed. Western-blot detection of P-Akt and P-eNOS expression levels in lung tissue was carried out. Nitrate reductase analysis was used to measure nitric oxide (NO) in lung tissue. Results: Genistein treatment resulted in significant improvement in the speed of tricuspid regurgitation, diameter of pulmonary artery, mean pulmonary artery pressure and right ventricular hypertrophy index. Genistein treatment also resulted in significant improvement in the stenosis of pulmonary artery, proliferation of smooth muscle, right ventricular hypertrophy and myocardial hypertrophy. These therapeutic effects were more obvious with increasing dose of genistein. After genistein treatment, amelioration
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    Metabolomics study of the formation of genotoxic molecules based on the fecal volatile metabolites profile using an in vivo animal model
    (Elsevier, 2024-02-09) Giménez Campillo, Claudia; Campillo Seva, Natalia; Arroyo Manzanares, Natalia; Torre-Minguela, Carlos de; Viñas López-Pelegrin, Pilar; Martínez Cáceres, Carlos Manuel; Química Analítica
    Epidemiological studies have shown that haem iron from processed meat is a key element involved in the colon carcinogenesis. The haem iron induces lipid peroxidation in the colon lumen during digestion, enabling the formation of cytotoxic molecules derived from these reactions. The cytotoxic molecules generated are highly dependent on dietary factors such as lipid sources, calcium levels or the presence of antioxidants during digestion. Here, we investigated whether nitrite substitution by polyphenols as a food additive could modulate the in vivo luminal lipid peroxidation in the colon and consequently, reduce the formation of mucin-depleted foci in a chemical-induced colon cancer rat model. The addition of polyphenols to the cooked ham diet reduces the lipid peroxidation in the rats. A reduction in cytotoxic aldehydes in fecal water from animals fed with polyphenols as well as a decrease in the formation of mucin-depleted foci is observed. The antioxidant capacity derived from polyphenols modifies the luminal environment of the colon, allowing the identification of a specific molecular signature derived from the analysis of fecal volatile organic compounds. In this molecular signature is included the reduction in the abundance of (2E,4E)-2,4-hexadienal, a carcinogenic aldehyde derived from lipid peroxidation.