Browsing by Subject "Oligosaccharides"

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    Distributional map of the terminal and sub-terminal sugar residues of the glycoconjugates in the prepubertal and postpubertal testis of a subject affected by complete androgen insensitivity syndrome (Morris’s syndrome): lectin histochemical study
    (Murcia : F. Hernández, 2004) Gheri, G.; Vannelli, G.B.; Marini, M.; Zappoli Thyrion, G.D.; Gheri, R.G.; Sgambati, E.
    In the present research we have investigated the distribution of the sugar residues of the glycoconjugates in the prepubertal and postpubertal testes of a subject with Morris’s syndrome (CAIS, Complete Androgen Insensitivity Syndrome). For this purpose a battery of six horseradish peroxidaseconjugated lectins was used (SBA, PNA, WGA, ConA, LTA and UEAI). We have obtained a complete distributional map of the terminal and sub-terminal oligosaccharides in the tunica albuginea, interstitial tissue, lamina propria of the seminiferous tubules, Leydig cells, Sertoli cells, spermatogonia, mastocytes and endothelial cells. Furthermore the present study has shown that a large amount of sugar residues were detectable in the prepubertal and postpubertal testes but that some differences exist with particular regard to the Sertoli cells. The Sertoli cells and the Leydig cells of the retained prepubertal testis of the patient affected by Morris’s syndrome were characterized by the presence of a-L-fucose, which was absent in the retained prepubertal testis of the normal subjects. Comparing the results on the postpubertal testis with those obtained on the same aged testis of healthy subjects we have demonstrated that a-L-fucose in the Sertoli and Leydig cells and D-galactose-N-acetyl-D-galactosamine in the results on the postpubertal testis with those obtained on the same aged testis of healthy subjects we have demonstrated that a-L-fucose in the Sertoli and Leydig cells and D-galactose-N-acetyl-D-galactosamine in the Leydig cells are a unique feature of the subject affected by Morris’s syndrome. D-galactose (ß1?3)-N-acetyl-Dgalactosamine and sialic acid, which are present in the Leydig cells of the normal testis were never observed in the same cells of the postpubertal testis of the CAIS patient.
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    Lectin binding in the human foetal testis
    (Murcia : F. Hernández, 2003) Gheri, G.; Vannelli, G.B.; Marini, M.; Zappoli Thyrion, G.D.; Sgambati, E.
    In the present study we have investigated the oligosaccharidic content of the glycoconjugates within the human foetal testis starting from its earliest differentiation phase (8, 10 and 12 weeks of gestation). To this purpose we have used a battery of six horseradish peroxidase-labelled lectins (SBA, PNA, WGA, UEAI, LTA and ConA). We have obtained a complete distributional map of the sugar residues of the glycoconjugates in the coelomic mesothelium, tunica albuginea, pre-Sertoli cells, pre-gonocytes, Leydig cells, basement membrane of the sex cords, interstitial tissue, mastocytes and endothelial cells of the capillary vessels. Since the beginning of the testis differentiation phase the cells of the coelomic mesothelium showed a large amount of sugar residues. In the pre-Sertoli cells and in the pre-gonocytes a role played as structural molecules by some oligosaccharides could be hypothesized. Dgalactose-( ß1?3)-N-acetyl-D-galactosamine, sialic acid, N-acetyl-D-glucosamine and a-D-mannose could be involved in inducing and maintaining the cellular activity of the Leydig cells.