Browsing by Subject "Olfactory epithelium"

Now showing 1 - 3 of 3
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    Developmental anatomy of the primary olfactory pathway in the opossum Monodelphis domestica
    (Murcia : F. Hernández, 1997) Chuah, M.I.; Tennent, R.; Teague, R.
    It has been shown in previous studies that the marsupial central nervous system is born at a relatively immature state. Although olfaction is thought to play a role in guiding the locomotion of the newborn, the cellular substrates on which this notion is based have not been systematically investigated. This review article surnmarises the anatomical development of the primary olfactory pathway in the postnatal Monodelphis. The olfactory epithelium and bulb appear morphologically immature at birth although some of the olfactory neurons are shown to express olfactory marker protein. The olfactory tissues subsequently undergo a rapid sequence of developmental events during the first two postnatal weeks. The evidence shows that the marsupial and eutherian olfactory system share a similar temporal sequence of developmental processes although the former proceeds at a lag time of about 10-14 days compared to that of mice (using the date of birth as a common reference point). Much physiological and behavioral studies remain to be done before we can be certain about the time at which full functionai maturity is attained in this system.
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    Distinct presence of the tight junction protein claudin-3 in olfactory bulb and fila olfactoria of the mouse
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Eppler, Elisabeth; Müller Gerbl, Magdalena; Maly, I. Piotr
    The tight junction protein claudin-3 is overexpressed in diverse epithelial tumours and is associated with increased survival, progression and motility of tumour cells. Claudin-3 expression profiles are being increasingly used for diagnostic and prognostic tumour classification. Claudin-3 has been identified as a receptor for Clostridium perfringens enterotoxin, which is under consideration for selective lysis of claudin-3- expressing tumours, particularly brain metastases, and other translational medicine uses. However, the localization of claudin-3 in the brain has not been completely elucidated. While claudin-3 in brain tissue adjacent to claudin-3-expressing metastases had been excluded and low or undetectable levels proposed in the CNS, under physiological conditions, in adult human, rat and mouse brains, claudin-3 was exclusively found in choroid plexus epithelium where it is considered an integral component of the blood-cerebrospinal-fluid barrier. We report here the pronounced presence of claudin-3 not only in the nasal region (as described for rat), but also in the mouse olfactory bulb and nerve using immunohistochemistry and Western blot. Claudin-3 was present in the fila olfactoria from the epithelium to the olfactory nerve and in the main and accessory olfactory bulb. We propose that the abundant presence of claudin3 in the olfactory system, particularly in nerve fibres and the olfactory bulb cone, which we present here, may play a role at the interface of the central and peripheral nervous system, both as barrier and for axonal growth and communication. Thus, claudin-3 should be considered and further explored with regards to treatment approaches addressing the olfactory bulb and nasal region.
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    Markers of senescence are often associated with neuronal differentiation in the developing sensory systems
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2023) Mera Rodríguez, José Antonio; Álvarez Hernán, Guadalupe; Gañán, Yolanda; Solana Fajardo, Jorge; Martín Partido, Gervasio; Rodríguez León, Joaquín; Francisco Morcillo, Javier
    It has been shown that senescent cells accumulate in transient structures of the embryo that normally degenerate during tissue development. A collection of biomarkers is generally accepted to define senescence in embryonic tissues. The histochemical detection of β-galactosidase activity at pH 6.0 (β-galpH6) is the most widely used assay for cellular senescence. Immunohistochemical detection of common mediators of senescence which block cell cycle progression, including p16, p21, p63, p15 or p27, has also been used to characterize senescent cells in the embryo. However, the reliability of this techniques has been discussed in recent publications because nonsenescent cells are also labelled during development. Indeed, increased levels of senescent markers promote differentiation over apoptosis in developing neurons, suggesting that machinery used for the establishment of cellular senescence is also involved in neuronal maturation. Notably, it has recently been argued that a comparable state of cellular senescence might be adopted by terminally differentiated neurons. The developing sensory systems provide excellent models for studying if canonical markers of senescence are associated with terminal neuronal differentiation.