Browsing by Subject "Microscopy"

Now showing 1 - 12 of 12
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    A comparative pathological study of three strains of Trypanosoma cruzi in an experimental model
    (Murcia : F. Hernández, 1991) De Diego, J.A.; Penin, P.; Del Rey, J.; Mayer, R.; Gamallo, C.
    Trypanosoma cruzi. the etiological agent of Chaps' cliscase. shows a wide variation in its biological hcl ia\ . io~~dre pending on the geographical distribution of different strains. Moreover. sonic. strains can show; variations with the course of tinic. We have studied the tissular tropism of three strains of T. cruzi, Cali. Buli\,ia and Y. from different geographical origins (Colombia. Bolivia ancl I3rasil respectively) on Swiss inice in order to detect any pnrsihle modification in their behaviour attributable only to parasite but not to host variations. The anatoniopathological stud!! of sections from heart. brain. liver. spleen. lymphatic ganglion. skeletal muscle and colon from Swiss mice infected with these strains has cvidencecl the presence of some important tliscrcpancics hctwecn the tissular tropism expected from thcir former clescriptions. and classical typification and then observed lesions. The greatest variations were found in the Y strain which had been described as eniincntly I-eticulotropic but presented lesions in all the organs except tlie spleen and lymphatic ganglion. We consider that the x~ariationsf ound in our study can only be explained in ternis of changes in the properties of the strains consiclercd. and conclude that the classic typificrrtion techniques based on the constant! of the characteristics of the parasite are not fully reliable for the description and clinical management of some evolving strains.
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    Anatomical and histochemical study of Sechium edule (Jacq.) Sw.
    (Universidad de Murcia. Servicio de publicaciones, 2020) de Andrade Silva, Francilainy Karina; dos Santos Magalhães, Cledson; Damasceno Sá, Rafaela; Lins da Silva, Flávia Carolina; Perrelli Randau, Karina
    Sechium edule (Jacq.) Sw. tiene un papel importante nutricional y terapéuticamente en la medicina popular, especialmente utilizado como agente hipotensor. Para ampliar la información farmacológica sobre esta especie, este trabajo tiene como objetivo el estudio histoanatómico de sus hojas. Se prepararon muestras con secciones transversales y paradérmicas de la hoja para el estudio microscópico. Para el análisis histoquímico, se utilizaron reactivos específicos para los metabolitos objetivo. A través del análisis realizado se identificaron estructuras anatómicas para la correcta identificación de la especie estudiada. La histoquímica mostró la presencia de alcaloides, triterpenos y esteroides, compuestos lipofílicos y lignina. Esta información sobre la localización de los compuestos no tiene precedentes. Así, el trabajo amplía el conocimiento sobre la especie y la familia Cucurbitaceae.
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    Anatomical and histochemical study of the stem and leaves of Jasminum azoricum L.
    (Universidad de Murcia. Servicio de publicaciones, 2023) Pereira, Hanna Karolyne de Melo; Magalhães, Cledson dos Santos; Randau, Karina Perrelli
    El presente estudio tuvo como objetivo caracterizar la anatomía e histoquímica del tallo y las hojas de Jasminum azoricum L. Para ello, se realizaron preparaciones histológicas semipermanentes que contenían cortes transversales de tallo y hoja, además de cortes paradérmicos de la lámina foliar, analizados por microscopía óptica. También se realizaron pruebas histoquímicas para verificar la ubicación de acumulación de constituyentes químicos en secciones transversales de la hoja. Las caracterizaciones anatómicas aportaron importantes datos para distinguir las especies y el estudio histoquímico reveló los sitios de síntesis y/o almacenamiento de los metabolitos que poseen importantes propiedades medicinales.
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    Cadmium- and lead-induced apoptosis in mallard erythrocytes (Anas platyrhynchos)
    (Elsevier, 2009-01) Romero García, Diego; Hernández-García, A.; Tagliati, C. A.; Martínez-López, E.; García Fernández, Antonio Juan; Ciencias Sociosanitarias
    Cadmium, lead and cadmium–lead (1:10) induced apoptosis were studied using mallard blood cells. The allowable range in concentrations were: 0.01–0.5, 0.1–5.0, and 0.01:0.10–0.50:5.00 mM, for cadmium, lead and cadmium–lead, respectively. The lowest EC50 achieved was for cadmium (0.2270.04 mM). Two doses from each treatment group were chosen to study apoptosis and the presence of metals in cells. The percentage of apoptotic cells increased as the concentration of metals increased. The percentage of cells with intracellular metals was high for both exposure levels and the quantity of intracellular metal was greater for exposure to high concentrations. Morphological alterations for all types of exposure were related to the diverse range of effects that these metals have on membranes. We suggest that the decrease in the number of erythrocytes observed in specimens suffering from lead and cadmium poisoning is related to the induction of apoptosis.
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    CD34+ stromal cells/fibroblasts/fibrocytes/telocytes as a tissue reserve and a principal source of mesenchymal cells. Location, morphology, function and role in pathology
    (F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología, 2014) Díaz-Flores, L.; Gutiérrez, R.; García, M. P.; Sáez, F.J.; Díaz-Flores Jr., L.; Valladares, F.; Madrid Cuevas, Juan Francisco
    We review the morphofunctional characteristics of CD34+ stromal fibroblastic/fibrocytic cells (CD34+ SFCs) and report our observations. We consider the following aspects of CD34+ SFCs: A) The confusing terms applied to this cell type, often combining the prefix CD34 with numerous names, including fibroblasts, fibrocytes, dendrocytes, keratocytes, telocytes and stromal, dendritic, adventitial, supraadventitial, perivascular, paravascular and delimiting cells; B) Changes in their immunophenotype, e.g., loss of CD34 expression and gain of other markers, such as those defining mesenchymal and derivate cells (myofibroblasts, osteoblasts, chondroblasts, adipocytes); C) Morphology (elongated or triangular cell body and thin, moniliform, bipolar or multipolar cytoplasmic processes), immunohistochemistry (co-expression of and changes in molecular expression) and structure (characteristics of nucleus and cytoplasmic organelles, and points of contact and junctions in quiescent and activated stages by light and electron microscopy); D) Location and distribution in the vessels (adventitia or external layer), in the tissues (connective, adipose, blood, muscle and nervous) and in the organs and systems (skin, oral cavity and oropharynx, respiratory, digestive, urinary, male, female, endocrine and lymphoid systems, serosal and synovial membranes, heart, eye and meninges); E) Origin from the mesoderm and cranial neural crest in the embryo, and from stem cells (themselves or other cells) and/or peripheral blood pluripotent stem cells (circulating progenitor cells) in post-natal life; F) Functions, such as synthesis of different molecules, progenitor of mesenchymal cells, immunomodulation, parenchymal regulation (growth, maturation and differentiation of adjacent cells), induction of angiogenesis, scaffolding support of other cells and phagocytic properties. Since CD34+ SFCs are the main reservoir of tissue mesenchymal cells (great mesenchymal potential, probably higher than that proposed for pericytes and other stromal cells), we dedicate a broad section to explain their in vivo behaviour during proliferation and differentiation in different physiologic and pathologic conditions, in addition to their characteristics in the human tissues of origin (adult stem cell niches); G) Involvement in pathological processes, e.g., repair (regeneration and repair through granulation tissue), fibrosis, tumour stroma formation and possible CD34+ SFC-derived tumours (e.g., solitary fibrous tumour, dermatofibrosarcoma protuberans, giant cell fibroblastoma, nuchal-type fibroma, mammary and extramammary myofibroblastoma, spindle and pleomorphic cell lipoma, and elastofibroma) and H) Clinical and therapeutic implications.
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    Comparison of cytopathological changes induced by mercury chloride exposure in renal cell lines (VERO and BGM)
    (Elsevier, 2004-03-31) Romero García, Diego; Gómez Zapata, Maximiliano; Luna, Aurelio; Ciencias Sociosanitarias
    The response to mercury chloride was assessed in two cell lines of renal origin, determining the range of toxic concentrations by Neutral Red assay after 24-h of exposure. Morphological changes in the Buffalo Green Monkey (BGM) and VERO cell lines after exposure to subcytotoxic doses (0.045 and 0.038 mM, respectively) equivalent to EC10 (effective concentrations 10%) of mercury chloride were evaluated at the structural and ultrastructural level by optic, transmission and scanning microscopy. Using transmission electron microscopy, the most notable findings in treated cells were the presence of intracytoplasmic inclusion bodies and apoptotic bodies. Scanning microscopy pointed to a cell with a disrupted perinuclear region and a decreased number of surface microvilli. Similar alterations in both in vivo and in vitro experiments have been described by other authors. We conclude that BGM and VERO renal cell lines can be considered as useful tools for toxicological studies involving mercury chloride.
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    Comprehensive analysis of different adhesives in aerobiological sampling using optical microscopy and high-throughput DNA sequencing
    (Elsevier, 2019-04-05) Rojo, Jesús; Núñez, Andrés; Lara, Beatriz; Sánchez-Parra, Beatriz; Moreno, Diego A.; Pérez-Badía, Rosa; Genética y Microbiología
    The standardization and unification of the procedures to analyze and quantify the airborne pollen concentrations are very important topics. In this work, the effectiveness of the two most used adhesives in aerobiological sampling, silicone prepared with cyclohexane solvent (Silicone) and petroleum jelly (Vaseline), was compared under outdoor conditions. This comparison was carried out using the traditional method based on the identification and quantification by optical microscopy (OM) of the airborne pollen and the novel methodology by high-throughput sequencing analysis (HTS). Globally, the results from both methods of analysis (OM and HTS) showed a good agreement between the two adhesives tested regarding the abundance of the main pollen types present in the samples: Cupressaceae, Olea, Poaceae, Platanus, Quercus. We concluded that the results from both adhesives are comparable data. Furthermore, the comparisons between methodologies, OM vs. HTS, showed that both techniques can accurately identify the most abundant pollen types in the atmosphere for the studied periods, with a good agreement of their relative abundances especially when the airborne pollen diversity is low but showing some divergences as the number of pollen types increases.
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    Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
    (Elsevier, ) Albaladejo-Riad, Nora; Espinosa-Ruiz, Cristóbal; Esteban Abad, María de los Ángeles; Biología Celular e Histología
    Silk fibroin microparticles (SF) were administered in the diet of gilthead seabream with or without experimental skin wounds to study the effects on cellular immunity and liver. A commercial diet was enriched with different SF contents: 0, 50 and 100 mg kg-1 (control, SF50 and SF100 diet, respectively). The animals were fed for 30 days and half of them were sampled. Similar experimental wounds were then performed to the rest of fish and they continued to be fed the same diet. At 7 days post-wounding, samples were taken from the wounded fish. Cellular immunity was studied on head kidney leucocytes (phagocytosis, respiratory and peroxidase content) and liver status (histological study and gene expression) were studied. Our results showed that experimental wounds affect both cellular immunity (by decreasing leucocyte respiratory burst and peroxidase activity) and altered liver histology (by inducing vascularization and congestion of blood vessels). In addition, it affects the expression of genes indicative of oxidative stress, endoplasmic reticulum stress and apoptosis. More specifically, it was the highest dose of silk fibroin (SF100) that significantly increased the phagocytic capacity of leucocytes as well as vascularization in the liver. In addition, increased expression of antioxidant genes (cat and gsr) and decreased expression of genes related to reticulum endoplasmic stress (grp94 and grp170) and apoptosis (nos and jnk) were detected in these fish fed with SF100 and wounded. In conclusion, fed fish with SF100 had many beneficial effects as cellular immunostimulant and hepatoprotection in wounded fish. Its use could be of great interest for stress management in farmed fish conditions.
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    Microscopic, histochemical and preliminary phytochemical characterization of leaves of Trema micrantha (L.) Blume
    (Universidad de Murcia, Servicio de Publicaciones, 2021) dos Santos Magalhães, Cledson; Damasceno Sá, Rafaela; Souto Maior de Araújo Baltar, Solma Lúcia; Perrelli Randau, Karina
    Caracterización microscópica, histoquímica y fitoquímica preilminar de las hojas de Trema micrantha (L.) Blume Para enriquecer el enriquecer el conocimiento sobre Trema micrantha (L.) Blume, esta investigación tuvo como objetivo realizar la caracterización anatómica, histoquímica y fitoquímica de las hojas de la especie. Se realizaron cortes transversales del pecíolo y limbo, así como cortes paradérmicos del limbo, analizados en microscopía óptica y polarizada. Se utilizaron diferentes reactivos para el análisis histoquímico. Se han descrito estructuras anatómicas que proporcionan un diagnóstico detallado de las especies estudiadas. La histoquímica mostró la presencia de metabolitos esenciales (flavonoides, taninos, entre otros) para la especie y mediante análisis SEM-EDS se confirmó que los cristales están compuestos por oxalato de calcio. El análisis fitoquímico permitió la identificación de mono y sesquiterpenos, triterpenos y esteroides, entre otros. El estudio proporcionó datos sin precedentes sobre la especie, ampliando la información científica de T. micrantha.
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    Morphoanatomical and histochemical study of Ipomoea hederifolia L. (Convolvulaceae)
    (Universidad de Murcia. Servicio de publicaciones, 2023) Vital dos Santos, Edinalva Alves; Nurit Silva, Kiriaki; Pereira de Arruda, Emília Cristina; Leite, Ana Virginia
    Ipomoea hederifolia L. is a herbaceous vine native to the tropical Americas with important medicinal properties. Was realized a pharmacobotanical study of the leaves and stems of this species, performing macroscopic and microscopic morphodiagnoses and histochemical tests. Anatomical characteristics typical of the family Convolvulaceae were found. However, the epidermis and its appendages (e.g. striated cuticle and peltate trichomes) and the anatomy of the petiole and the stem presented relevant characters for the taxonomic recognition of the species. Histochemical tests evidenced the presence of lignin and cutin and positive reactions for starch, phenolic compounds, and proteins. The anatomy and the histochemical tests indicated a set of characteristics relevant to the pharmacobotanical characterization of I. hederifolia, expanding our knowledge of the species and providing subsidies for the quality control of its vegetal products.
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    The multilayered structure of the human corpus spongiosum
    (2018) De Graaf, P.; Ramadan, R.; Linssen, E.C.; Staller, N.A.; Hendrickx, A.P.A.; Pigot, G.L.S.; Meuleman, E.J.H.; Bouman, M.; Özer, M.; Bosch, J.L.H.R.; de Kort, L.M.O.
    Purpose. Urethral reconstruction is performed in patients with urethral strictures or for correction of congenital disorders. In the case of shortage of tissue, engineered tissue may enhance urethral reconstruction. As the corpus spongiosum (CS) is important in supporting the function of the urethra, tissue engineering of the urethra should be combined with reconstruction of a CS. For that purpose, detailed knowledge of the composition of the CS, more specifically its extracellular matrix (ECM) and vascularization is needed for scaffold design. The objective of this study is to analyze the microarchitecture of the CS through (immuno) histology and scanning electron microscopy (SEM). Methods. The CS including the urethra of patients undergoing male-to-female genital confirming surgery was harvested. This CS was fixed and processed for either (immuno) histology or for SEM. Results. Four layers could be distinguished in the CS; first a transition zone from urethra epithelium to a collagen rich layer, which was highly vascularized, followed by a second, elastin rich layer. The third layer was formed by veins, arteries and vascular spaces and the last layer showed the transition from this vascular rich region to the collagen rich tunica albuginea. In this layer collagen bundles intertwined with elastic fibres. In the CS different components of the ECM were visible and distinguishable. Conclusion. This study provides novel and detailed information on the microarchitecture of the CS and the distribution of vascularization, which is important for scaffold design in tissue engineering.
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    Unusual circular annulate lamellae in hepatocytes of Torpedo marmorata
    (Murcia : F. Hernández, 2005) Haggag, A.; Gilloteaux, J.
    This report describes an unusual morphology of annulate lamellae (AL) in the hepatocytes of Torpedo marmorata Risso. These Als and fragments are detected amidst the main glycogen and lipid deposits. AL cisterns are circumscribed by parts of the smooth endoplasmic reticulum. Based on the finding of these unusual annular ALs, accompanied by other subcellular lesions such as a number of membranous whorls and altered mitochondria. These findings can concur and support other authors’ observations suggesting that these adult hepatocytes transient changes reflect that this species could be exposed to local, natural or likely human coastal seabed pollutants.