Browsing by Subject "Mast cell"

Now showing 1 - 3 of 3
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    Estudio anatomopatológico y mastocitos en hígados de ternero decomisados en matadero
    (Universidad de Murcia. Servicio de publicaciones, 2022) Bas Murcia, Ana de los Ángeles; Gómez Sánchez, Miguel Ángel; Bernabé Salazar, Antonio; Sánchez Martínez, Pedro
    Este estudio aborda las alteraciones anatomopatológicas y el número de mastocitos en 51 hígados de ter-nero decomisados en un matadero importante del sur-este de España. El análisis microscópico se ha realizado con las tinciones hematoxilina-eosina (H-E) y Tricrómico de Masson (TRC). Además, se determinó el núme-ro de mastocitos en función de sus patologías, mediante la tinción azul de toluidina, haciendo un conteo en 10 campos de 348 x 263 μm a 40x en cada una de las muestras. Con el programa estadístico SPSS y las pruebas estadísticas Kolmogorov-Smirnov y Mann-Whitney se ha hecho una comparación con 5 hígados control. Las lesiones anatomopatológicas causantes de decomiso han sido: abscesos (47%), colangiohepatitis (31%), fibrosis (8%), esteatosis (6%), atrofia (2%), necrosis (2%), congestión (2%) y quiste (2%). Clasificándose en lesiones inflamatorias (49%), lesiones parasitarias (33%) y lesiones degenerativo-metabólicas (18%). Los re-sultados del conteo de mastocitos muestran que hay diferencias significativas en el número de mastocitos de los hígados con colangiohepatitis (p 0,001), respecto a los hígados control y no hay diferencias significativas en los hígados con abscesos (p 0,227). Al hacer una clasificación por familias de las alteraciones anatomopa-tológicas estudiadas se ha llegado a la conclusión de que no hay diferencias significativas en las alteraciones inflamatorias al compararlas con el grupo control y sí las hay en las alteraciones parasitarias. Las alteraciones degenerativas también muestran diferencias significativas, pero el número de muestras recogidas es bajo.
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    Mast cells or not? - CD117 positive cells in esophageal leiomyoma
    (F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 2015) Ye, Ju Xiang; Liu, Yan; Qin, Yun; Ma, Xiao Long; Zhong, Hao Hao; Zhang, Yan; Shi, Xue Ying
    The presence of CD117 positive cells in esophageal leiomyoma may lead to a misdiagnosis of GIST. We reviewed 46 esophageal tumors which were smooth muscle tumors or GIST. Based on morphology, immunohistochemistry and mutation analysis, there were 44 (95.6%) leiomyomas, 1 (2.2%) leiomyosarcoma, and 1 (2.2%) GIST. Variable numbers of CD117 positive cells were seen in all leiomyomas. Tryptase immunostaining identified mast cells in 93.2% (41/44) of leiomyomas, and the number of mast cells per tumor decreased significantly from tumors of the upper esophagus to the esophageal-gastric junction (p<0.01). Immunofluorescence study further confirmed the presence of two types of CD117 positive spindle cells which included spindle-shaped mast cells and DOG-1- positive interstitial cells of Cajal. This is the first study to systemically review mast cells in esophageal leiomyomas and tumors which may be included in the differential diagnosis. We demonstrate that both spindled mast cells and hyperplastic interstitial cells of Cajal are present within esophageal leiomyomas. The immunoreactivity of these cells with CD117 may suggest a diagnosis of GIST, but the presence of mast cells itself supports a diagnosis of esophageal leiomyoma.
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    Pathology and terminology of interstitial cystitis/bladder pain syndrome: A review
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Akiyama, Yoshiyuki; Homma, Yukio; Maeda, Daichi
    Interstitial cystitis/bladder pain syndrome (IC/BPS) is an umbrella term of chronic debilitating conditions of unknown etiology characterized by symptoms of lower urinary tract hypersensitivity such as bladder pain/discomfort, urgency, and urinary frequency. The pathological features of IC/BPS have been generally reported as non-specific chronic inflammatory changes, with mast cell infiltration as a potential key finding. However, growing evidence reveals a histological distinction between IC/BPS with Hunner lesions and IC/BPS without Hunner lesions, and also sheds doubt on the diagnostic value of the mast cell count. Specifically, IC/BPS with Hunner lesions is an inflammatory disorder characterized by pancystitis with B cell abnormalities and epithelial denudation, while IC/BPS without Hunner lesions shows minimal histological changes. The umbrella term “IC/BPS” connects totally distinct clinical entities. Pathological evaluation thus plays an important role in the precise subtyping and clinical management of IC/BPS. In addition, terminology should be developed to refer separately to IC/BPS with Hunner lesions and IC/BPS without Hunner lesions