Browsing by Subject "Lymphoid follicle"

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    lmmunophenotypic characterization of primary and secondary lymphoid follicles
    (Murcia : F. Hernández, 1988) Luaces, César P. de; Peral, José I.; García Tejeiro, Manuel; Aguirre, César
    Thc need for an immunophenotypical referential framework relative to lymphoid follicle has led us to apply a panel of monoclonal and polyclonal antibodics, by means of a sensitive immunostaining method. Lymphoid follicle is an immunophenotypically complex structure made up of three lymphoid populations (B, being its bulk, and a few T and NK cells). dcndritic reticulum cells (DRCs) and Flemming's macrophagcs. Follicular B population is To 15 +. B1 + , OKB 7 +, HLA-DR + and C3bR +. In secondary follicles therc are differential characteristic reactivities for cach topographic compartment: Mantle zone is positive for OKB 2 and surface IgM (sIgM) and IgD (sIgD): germinal center (GC) clear zone (with centrocytic predominance) for OKT 9, sIgM and weakly for OKB 2: and GC dark zone (with centroblastic predoiniiiance) only for OKT 9. In sections, OKT 10 allows one to see immunoblasts and plasma cells, the latter being with lymphoplasmacytoid cells the only intracytoplasmic immunoglobulin holders. 10% of GC lympliocytcs are T cells, almost exclusively T-helper (Leu 3a +). Another 10% to 15% of lymphoid cells are Leu 7 (HNK- 1) +. In histological sections, DRCs are spccifically marked with R4123 and Flemming's inacrophages with anti-alpha,-antitrypsin and antialpha,- antichymotrypsin antibodies, both populations bcing negative to OKM 1 and OKM 5.
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    The lymphocyte-dendritic cell system
    (Murcia : F. Hernández, 1998) Imai, Yutaka; Yamakawa, Mitsunori; Kasajima, Takeshi
    Antigens provoke immune responses. The group of immunocompetent cells related directly to this response includes T and B cells, macrophages (MO) and dendritic cells (DCs). DCs acting as antigen-presenting cells have been recently recognized to be important in initiating the immune response. B cells and follicular dendritic cells (FDCs), the major immunocompetent cells in the B-cell dependent area, play an important role in humoral immunity, while T cells and interdigitating cells (IDCs), which are the major immunocompetent cells in the T-cell dependent (TD)-area, play an important role in cellular immunity. The B cell-IDC interaction in the TD-area is also essential for the B-cell response against TD-antigen. Consequently, the lymphocyte-DC interaction is essential in the response to antigenic stimulation and in inducing the potent effector cells. B cell-DC, T cell-DC and DC-B cell-T cell interactions are regulated in predetermined sites by complex and varied mechanisms. Much recent evidence demonstrates that DCs modulate lymphocyte biology in its broadest aspects, including generation, differen-tiation, proliferation, and activation. In this review, we outline recent studies on the generation, structure, and function of lymphatic tissues, propose the concept of the "Lymphocyte-Dendritic Cell System (LDS)", and finally describe the significance and functions of this system in health and disease.