Browsing by Subject "Haptoglobin"

Now showing 1 - 6 of 6
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    Acute phase protein concentrations in retired racing Greyhounds
    (Wiley, 2009-06) Guillermo Couto, C.; Cerón Madrigal, José Joaquín; Parra Muñoz, María Dolores; Martínez-Subiela, Silvi; Lazbik, M. Cristina; Ciencias Sociosanitarias
    Background: Retired racing Greyhounds are popular as pets. Greyhounds have several differences in physiological values compared with other breeds, including lower serum a- and b-globulin concentrations. We hypothesized that lower acute phase protein (APP) concentrations could contribute to lower a- and b-globulin concentrations in this breed. Objectives: The purpose of this study was to compare serum concentrations of several APPs in Greyhounds with those of other dog breeds. Methods: We measured the serum concentrations of C-reactive protein (CRP), haptoglobin (Hp), acid-soluble glycoprotein (ASG), ceruloplasmin (CP), and serum amyloid A (SAA) in 15 clinically healthy retired racing Greyhounds and 11 age- and gender-matched healthy nonGreyhound controls using previously validated methods. Results were compared by Student’s t-tests. Results: The concentration of Hp by both colorimetric and immunoturbidimetric methods was significantly lower in Greyhounds than in nonGreyhound dogs (P =.0009 and .019, respectively). The concentration of ASG was also significantly (P =.007) lower in Greyhounds, but CRP and CP concentrations were not significantly different between groups. SAA concentration was below the detection limit of the method in all dogs. Conclusions: The low serum concentrations of Hp and ASG should be taken into consideration when interpreting APP results in Greyhounds. Because both Hp and some ASG migrate in the a-globulin fraction, these results may explain the low a-globulin concentrations in Greyhounds.
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    Analytical validation of commercially available methods for acute phase proteins quantification in pigs
    (Elsevier, 2006-12-01) Tecles Vicente, Fernando; Fuentes, P.; Martínez Subiela, Silvia; Parra Muñoz, María Dolores; Muñoz, A.; Cerón Madrigal, José Joaquín; Ciencias Sociosanitarias
    The aim of this study was to validate commercially available methods for porcine haptoglobin (Hp), C-reactive protein (CRP), serumamyloid A (SAA) and major acute phase protein (Pig-MAP) determinations. Intra and inter assay coefficients of variation (CVs) were lower than 20% in all cases with exception of inter assay CVs for CRP and Pig-MAP assays with samples of low acute phase proteins concentration, and for SAA assay at any acute phase proteins concentration. All methods showed good linearity and detection limits were low enough to detect APPs levels in healthy animals. Hp and SAA were very affected by haemolysis. Lipaemia influenced mainly on SAA determination. Over 15-fold increase was observed in CRP and SAA concentrations after artificially induced inflammation by a single subcutaneous dose of turpentine, whereas Hp and Pig-MAP increased less than 5-fold.
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    Changes in serum biomarkers of inflammation in bovine besnoitiosis
    (2021-09-22) González Barrio, David; Huertas López, Ana; Diezma Díaz, Carlos; Ferre, Ignacio; Cerón Madrigal, José Joaquín; Ortega Mora, Luis Miguel; Álvarez García, Gema; Medicina y Cirugía Animal
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    Development of a time-resolved fluorometry based immunoassay for the determination of canine haptoglobin in various body fluids
    (INRA / EDP Sciences, 2005) Parra Muñoz, María Dolores; Väisänen, Ville; Cerón Madrigal, José Joaquín; Ciencias Sociosanitarias
    A time-resolved immunofluorometric assay (TR-IFMA) was developed for the determination of haptoglobin (Hp) in canine serum. Haptoglobin was purified from canine acute phase serum by ammonium sulphate precipitation followed by gel filtration. This isolated dog Hp was used as the standard to calibrate the assay. Intra- and inter-assay coefficients of variation of the assay were, respectively, 5.7% and 16.6% at 0.51 mg/mL, 2.4% and 10.6% at 2.1 mg/mL and 10.5% and 11.9% at 32.5 mg/mL. The dilution of serum samples with high Hp concentrations resulted in linear regression equations with R2 of 0.99 and 0.97. A high correlation was found in serum Hp measurements by TR-IFMA and a commercial assay based on peroxidase activity of haemoglobin bound to haptoglobin (R2 = 0.96). The limit of detection for the TR-IFMA method was 0.002 µg/mL. The addition of fresh haemolysate to serum samples did not affect the haptoglobin concentration (P = 0.694). Statistical differences (P < 0.003) were found between healthy dogs and dogs with different pathological processes. In whole blood, Hp concentrations were much lower than in serum but closely related (R2 = 0.84) whereas saliva Hp concentrations were poorly related with serum concentrations (R2 =0.53). However, the concentration of Hp in saliva was significantly (P < 0.039) higher in dogs with pathological processes compared to healthy dogs. The assay sensitivity was adequate to also be applied to whole blood and saliva specimens.
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    Serum acute phase response induced by different vaccination protocols against circovirus type 2 and Mycoplasma hyopneumoniae in piglets
    (Elsevier, 2017-03-11) Hernandez-Caravaca, Iván; Figueras Gourgues, Sebastian; Rodríguez, Víctor; Díaz Estrada, Edgar; Cerón Madrigal, José Joaquín; Escribano Tortosa, Damián; Producción Animal
    The purpose of this study was to compare the acute phase reaction (APR) induced by different vaccination pro- tocols used against Porcine Circovirus (PCV) type-2 and Mycoplasma hyopneumoniae (M.hyo), studying two acute phase proteins (APPs) and changes in rectal temperature (RT). In addition, the possible influence of the time of vaccination and breed were analysed. In the first experiment, 40 commercial crossbred piglets were vaccinated, on the day of weaning, with FLEXcombo® (group A, n = 20) or Porcilis PCV® and Stellamune® One (group B, n = 20). The second experiment was performed on two farms, on which 40 commercial crossbred piglets or 40 Iberian piglets were vaccinated, 7 days post-weaning. On each farm one group (A, n = 20) was vaccinated with FLEXcombo® and another group (B, n = 20) with Porcilis® PCV-M.hyo. Blood samples were taken before, 24 h and 48 h after vaccination, and RT were recorded before and 8 h after vaccination. Significantly higher in- creases in group B in RT (P b 0.01) and APPs concentrations (P b 0.01) were recorded at several sampling times after vaccination compared with group A. The vaccines that produced greater increases in RT also produced higher APPs increases but no influence of the day of vaccination or of the breed were found. Therefore, serum APPs concentrations differed according to the vaccine used, which may be useful, along with RT, for choosing the vaccine or protocol that produces APR of lower magnitude.
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    Yolkin tempers inflammatory mediator release and liver pathology in experimental endotoxemia in mice
    (2026) Jolanta Artym; Maja Kocięba; Ewa Zaczyńska; Kaleta-Kuratewicz; Jan P. Madej; Piotr Kuropka; Aleksandra Zambrowicz; Łukasz Bobak; Michał Zimecki; Biología Celular e Histología; Universidad de Murcia, Departamento de Biologia Celular e Histiologia
    Yolkin is an egg yolk-derived protein with immunoregulatory properties. In this work, yolkin was evaluated as a protective agent in endotoxemic BALB/c mice. The mice were pretreated with yolkin either orally in drinking water or intraperitoneally (i.p.) before i.p. injection of E. coli lipopolysaccharide (LPS). Circulating blood leukocyte number, blood cell composition, serum levels of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and haptoglobin, as well as histological changes in the spleen and the liver, were examined. Yolkin differentially regulated the values of these parameters, depending on the administration protocol; however, the serum levels of TNF-α and IL-6 were generally decreased, and the level of haptoglobin, an acute-phase protein, was elevated. The pretreatment of mice with yolkin led to improved histological architecture in the investigated organs of endotoxemic mice, particularly in the liver, where yolkin diminished an increased level of vascular permeability and reversed a decreased number of Kupffer cells. These changes were independent of the route of yolkin administration. In conclusion, yolkin proved effective in the amelioration of pathogenic consequences of LPS administration and may be considered a potential protective measure for patients at risk of endotoxemia. Histol Histopathol