Browsing by Subject "Cellular immunity"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Publication
    Open Access
    Foreign serum-induced bile duct lesion BDL in athymic BALBIc nude mice
    (Murcia : F. Hernández, 2000) Honjo, K.; Doi, K.
    To investigate a role of cellular immunity in foreign serum-induced bile duct lesion (BDL) in mice, athymic BALB/c nude (nu/nu) mice were intraperitoneally injected with swine serum (SS) twice a week up to 8 weeks and were compared with euthymic BALB/c heterozygote (nu/+) and wild-type (+/+) mice treated with SS in the same way for 4 weeks. All immunized nu/+ and +/+ mice developed marked BDL, and their sera showed high anti-SS IgE and IgGl antibody titers, whereas no immunized nulnu mice developed lesions, and their sera showed no elevation of antibody titers. Next, nu/nu mice were reconstituted with splenocytes derived from nu/+ mice, and then were intraperitoneally injected with SS twice a week for 3 weeks. Most of the reconstituted nulnu mice developed BDL, and their sera showed the elevation of anti-SS IgE and IgG antibody titers. These results suggest that cellular immunity may play a pivotal role in the pathogenesis of swine serum-induced BDL.
  • Thumbnail Image
    Publication
    Open Access
    Hepatic macrophage activation and the LPS pathway in patients with different degrees of severity and histopathological patterns of drug induced liver injury
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2021) Du, Hui-juan; Zhao, Su-xian; Zhao, Wen; Fu, Na; Li, Wen-cong; Qin, Xiao-jie; Zhang, Yu-guo; Nan, Yue-min; Zhao, Jing-min
    Background. Inflammatory activation of hepatic macrophages plays a primary role in druginduced liver injury (DILI). However, the exact mechanism underlying DILI remains unclear. Methods. A total of 328 DILI patients and 80 healthy individuals were prospectively enrolled in this study. The DILI patients were categorized into subgroups based on either disease severity or histopathological patterns. Plasma soluble CD163 (sCD163) and hepatic CD163 were examined to determine hepatic macrophage activation, and CD8, CD20, and MUM-1 were assessed to determine cellular immunity using immunohistochemistry. The lipopolysaccharide (LPS) pathway proteins [e.g. LPS, soluble CD14 (sCD14), and LPSbinding protein (LBP)] were measured using enzymelinked immunosorbent assay. Results. Plasma sCD163 levels were nine-fold higher in DILI patients than in healthy controls at the baseline, but significantly decreased at the 4-week follow-up visit after treatment. The numbers of hepatic macrophages, B cells, and plasma cells were significantly higher in the liver tissues from DILI patients than those from healthy controls. Furthermore, the baseline levels of LPS pathway proteins in the DILI patients were significantly higher than those in the controls. Notably, these proteins significantly decreased at the 4-week follow-up visit but remained significantly higher than the levels for the controls. Conclusions. Hepatic inflammation in DILI involves the activation of hepatic macrophages and cellular immunity, in which the LPS pathway likely plays a role, at least in part. As such, this study has improved our understanding of the pathological mechanisms for DILI and may facilitate the development of better treatments for patients with DILI.
  • Repository logo
    Publication
    Metadata only
    Mononuclear cell infiltrate, HLA-Dr expression and proliferation in 37 acoustic schwannomas
    (Murcia : F. Hernández, 1990) Rossi, M.L.; Jones, N.R.; Esiri, M.M.; Havas, L.; Nakamura, N.; Coakham, H.B.
    Frozen sections from 37 schwannomas of the V111 nerve were reacted with a panel of monoclonal antibodies to macrophage, lymphocyte, I-ILA-Dr invariant chain and nuclear proliferation antigens. A moderate number of rnacrophages was demonstrated in 96% of tumours. CD8- and CD4- lymphocytes were detected in slightly smaller numbers in up to 87% and 23% of tumours respectively. B-lyrnphocytes were present in only 2/32 cases and NK-cells were absent from all 16 cases tested. HLA-Dr antigen was expressed by macrophages in most cases and by tumour cells in 13/24 tumours. These findings may represent evidence for a degree of cellular immune response. Occasional cells featuring nuclear proliferation were detected in 15/27 cases.