Histology and histopathology Vol.40, nº7 (2025)
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- PublicationOpen AccessGradual expression of MMP9 and MT1-MMP at the tumor-stroma interface in head and neck squamous cell carcinoma(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Rusu, Stefan; Nuyens, Vincent; Rousseau, Alexandre; Lothaire, Philippe; Nagy, Nathalie; Boudjeltia, Karim Zouaoui; Uzureau, Pierrick; Biología Celular e HistologíaDue to the late-stage diagnosis of head and neck squamous cell carcinoma (HNSCC), treatment remains a significant clinical challenge. The metallo-proteinases MMP-9 and MT1-MMP play a pivotal role in extracellular matrix remodeling, thereby facilitating tumor growth and metastasis. Tumor progression requires the degradation of the basement membrane. The principal components of this structure, namely collagen IV and laminin, are the main targets of both MMP-9 and MT1-MMP. However, they can also exert influence over the expression of these enzymes. Oxidative stress plays an instrumental role in tumor development, functioning as a key inducer of metalloproteinase expression. The present study investigates the distribution of MMP-9 and MT1-MMP within tumor nests and along the basement membrane, comparing these with the distributions of collagen IV, laminin-332, and the antioxidant MnSOD. Biopsies from 12 patients with HNSCC and poor prognostic factors were subjected to immuno-fluorescence analysis. MMP-9 and MT1-MMP were found to be predominantly present in tumor cells, with a significant decrease in expression from the periphery to the center of tumor nests. Co-localization studies with laminin-332 and collagen IV, revealed substantial overlap, in accordance with the role of MMPs in basal membrane degradation. The cellular expression of laminin-332 associated with MMP-9 expression suggests an intricate relationship between metalloproteinases and their targets. While the previously observed pattern of glutathione-producing enzyme was similar to the metalloproteinases pattern, MnSOD expression was homogeneously distributed within tumor nests. Our findings reveal various distribution patterns of oxidative stress regulators, suggesting a complicated interplay in the development of HNSCC
- PublicationOpen AccessBiomechanical and histological analyses of a multilayer stent in a swine model of suprarenal aortic aneurysm(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Tobita, Allana Maryel; Strazzi, Anna Paula Weinhardt Baptista; Portugal, Maria Fernanda Cassino; Wolosker, Nelson; Aun, Ricardo; Monteiro, Frederico de Lima Jacy; Vieira da Silva, Luiz; Sincos, Igor Rafael; Biología Celular e HistologíaObjectives. To analyze and compare, in an animal model, the treatment of thoracoabdominal aneurysms with multilayer stents and its hemodynamic effects through the biomechanical and histological analysis of the aortic wall in contact with the stent. Methods. Large White pigs were randomized into two groups: Stent (n=6) and Control (n=5, non-stent). All animals were subjected to the creation of a suprarenal aneurysm with a bovine pericardial patch. In the Stent group, a multilayer stent was implanted immediately after aneurysm formation. After four weeks, all animals were subjected to angiographic assessment and intravascular ultrasound, and the stent was explanted before euthanasia for histological and biomechanical analyses. Results. At histological analysis, the groups did not differ significantly in maximum thickness of the intima (p=0.526), media (p=0.129), or adventitia (p=0.662). Thrombus formation was observed in 100% of the animals on the intima and media layers of the stented aorta vs. none in the Control group (p=0.048). At biomechanical analysis, no statistical differences were observed in aortic wall elasticity (p=0.158), strength (p=0.360), or thickness (p=0.323). Conclusion. We identified thrombosis of the aneurysmal sac through the presence of thrombi on the intima of the aorta in 100% of the animals in the Stent group; as for the biomechanical analysis, this study showed no statistical differences in vessel wall thickness, strength, and elasticity between
- PublicationOpen AccessThe methyltransferase KIAA1429 potentiates cervical cancer tumorigenesis via modulating LARP1 mRNA m6A modification and stability(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Feng, Xi; Shu, Liuping; Biología Celular e HistologíaCervical cancer (CC) is one of the most common gynecological malignancies in the world and poses a great threat to public health. There is inadequate knowledge of the molecular mechanisms underlying CC. This study aimed to explore the prognostic value of KIAA1429 (VIRMA, vir-Like m6A methyltransferase associated) in patients with CC and analyze its molecular mechanisms. The level of KIAA1429 in tumor specimens was tested using RT-qPCR and western blotting. Cellular biological processes were assessed using CCK-8 and Transwell assays. Xenograft experiments were used to verify the function of KIAA1429 in CC in vivo. The results manifested that KIAA1429 expression was enhanced in CC. Downregulation of KIAA1429 hindered the viability, migration, and invasion of CC cells. Moreover, LARP1 (La-related protein 1) was uncovered to be positively modulated by KIAA1429. Further, the anti-tumor impacts of KIAA1429 depletion on the phenotype of CC cells were counteracted by LARP1 amplification. Additionally, KIAA1429 deficiency suppressed the stability of LARP1 through methylating LARP1. Collectively, KIAA1429 can boost the tumorigenesis of CC via modifying LARP1 through m6A methylation to promote its stability. This work highlights the promoting effects of KIAA1429 on CC development and presents new targets for its treatment.
- PublicationOpen AccessDNMT1 silencing induces KIR2DL1/2/3 expression via methylation to alleviate graft-versus-host disease after allogeneic hematopoietic stem cell transplantation(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Zhang, Ping; Yu, Shuling; Yan, Xiao; Zhu, Huiling; Sheng, Lixia; Zhang, Yi; Yang, Shujun; Ouyang, Guifang; Biología Celular e HistologíaNatural killer (NK) cells are the promoters in graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT), while demethylation can regulate NK cell function. We explored the mechanism of demethylation regulating NK cell function to affect GVHD after allo-HSCT. BALB/c mice were transfused with C57BL/6 mouse-derived NK and bone marrow cells to establish GVHD models, followed by isolation and in vitro expansion of NK cells. NK cell purity, cytokine levels, proliferation, and cytokine-producing NK cell levels were measured via flow cytometry. KIR2DL1/2/3 methylation was tested by Methylation-specific polymerase chain reaction (MSP), with determination of mouse survival and GVHD scores. KIR2DL1/2/3 and DNMT1 expression was detected through qRT-PCR and/or western blot. Methylation levels were upregulated and KIR2DL1/2/3 expression was downregulated in GVHD mouse model-derived NK cells following IL-2 stimulation. DNMT1 silencing promoted KIR2DL1/2/3 expression, proliferation, and the secretion of Granzyme, Perforin, and Interferon-γ (IFN-γ) in C57BL/6 mouse-derived NK cells. DNMT1 silencing also enhanced mouse survival, reduced GVHD scores, promoted KIR2DL1/2/3 expression on the NK cell surface, and increased the secretion of Granzyme, Perforin, IFN-γ, and the number of cytokine-producing NK cells in the spleen, liver, and lung tissues of the models. Collectively, DNMT1 silencing induced KIR2DL1/2/3 expression in NK cells through reducing methylation to alleviate GVHD after allo-HSCT
- PublicationOpen AccessTesticular cryopreservation: From technical aspects to practical applications(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Pereira, Ana Glória; Matos, Tayná Moura; Albuquerque, Joana Letícia Cottin de; Silva, Andréia Maria da; Silva, Alexandre Rodrigues; Biología Celular e HistologíaTesticular cryopreservation has been highlighted as a promising alternative for preserving male fertility and can be applied to restore spermatogenesis in prepubertal individuals or cancer patients, preserve biologically valuable genotypes, and in studies on reproductive physiology or toxicity of various substances. This review presents an analysis of the technical aspects and applications of testicular cryopreservation, examining the contributions of important studies in this area and discussing the different factors that can impact the efficiency of the technique. Testicular fragments can be obtained from living or dead individuals, at any age and reproductive stage, through orchiectomy or biopsy. Among the methods used for processing, slow freezing and vitrification in open or closed systems stand out. However, factors such as species, age, medium used, cryoprotectants, and cryopreservation method can influence the viability of the testis after heating. To obtain sperm, the testes can be cultured in vitro or in vivo and the recovered gametes applied in assisted reproduction techniques. However, in some species, mainly wild animals and humans, this is still a limitation to be overcome.
- PublicationOpen AccessPhosphorylated protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK) expression in breast cancer is correlated with malignant proliferation and histological grading(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Yang, Xiaosi; Duan, Shuqiang; Zha, Jie; Jiang, Tao; Ye, Chun; Yu, Shuihong; Biología Celular e HistologíaThis study aims to detect the expression of phosphorylated PERK in breast cancer using immunohistochemistry and explore its significance. We examined 134 cases of formalin-fixed and paraffin-embedded breast cancer tissues. It was found that the expression of phosphorylated PERK in ductal carcinoma was higher than that in lobular carcinoma, and the difference between them was statistically significant, suggesting that phosphorylated PERK played different roles in the occurrence and development of different types of breast cancer. Compared with Ki-67-negative breast cancer tissues, phosphorylated PERK has higher expression in Ki-67-positive tissues and is positively correlated with Ki67 expression, indicating that phosphorylated PERK plays an important role in breast cancer's malignant proliferation and progression. We also found a positive correlation between phosphorylated PERK expression and the histological grading of invasive ductal carcinoma, indicating that phospho-rylated PERK plays an important role in the differentiation of invasive ductal carcinoma. Our study revealed the differential expression of phosphorylated PERK in subtypes of breast cancer. It contributed to the malignant proliferation of breast cancer and tissue differentiation of invasive ductal carcinoma of the breast.
- PublicationOpen AccessExploring pathological targets and advancing pharmacotherapy in autism spectrum disorder: Contributions of glial cells and heavy metals(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Chatterjee, Dhrita; Maparu, Kousik; Singh, Shamsher; Biología Celular e HistologíaAutism spectrum disorder (ASD) is a globally recognized neurodevelopmental condition characterized by repetitive and restrictive behavior, persistent deficits in social interaction and communication, mental disturbances, etc., affecting approximately 1 in 100 children worldwide. A combination of genetic and environmental factors is involved in the etiopathogenesis of the disease, but specific biomarkers have not yet been identified. Due to the lack of clinical evidence, fluctuations in symptoms, and difficulties in in-vitro and in-vivo modeling, developing medications for ASD is quite difficult. Although several drugs are used to treat autism, only risperidone and aripiprazole have received FDA approval in the United States. Epidemiological studies have suggested that maternal exposure to valproic acid (VPA), acetaminophen, propionic acid, and metals, such as cadmium (Cd), lead (Pb), arsenic (As), and mercury (Hg), may contribute to the development of various neurodevelopmental disorders. Pathological targets directly implicated in the disease include excitatory-inhibitory (E/A) imbalance, hyperserotonemia, GSK-3 inhibition, and Akt pathway activation. However, while a combination of pharmacotherapy, behavioral, and nutritional/dietary interventions has been found to be the most effective conventional therapy to date, many patients have chosen to implement particular dietary supplements for reducing ASD symptoms. In this review, we briefly describe various pathological targets and their roles in the pathophysiology of ASD and treatment strategies, including some future research directions.
- PublicationOpen AccessFortunellin attenuates sepsis-induced acute kidney injury by inhibiting inflammation and ferroptosis via the TLR4/NF-κB pathway(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Tianzhi, Liu; Yanmin, Zhang; Liu, Xiujuan; Zuo, Zhigang; Biología Celular e HistologíaObjective. To investigate the potential protective effect of fortunellin in sepsis-induced acute kidney injury (AKI) and its underlying mechanisms. Methods. Lipopolysaccharide (LPS)-treated human kidney proximal tubular epithelial (HK-2) cells were used as a cell model and sepsis-induced AKI was induced by cecal ligation and puncture (CLP) surgery in mice. Cell Counting Kit-8 (CCK8) assays and flow cytometry analysis were performed to examine the viability of HK-2 cells. Enzyme-linked immunosorbent assay (ELISA) was performed to investigate the content of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) in vivo and in vitro. The levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), and free iron (Fe2+) were measured as indicators of ferroptosis. The phosphorylation levels of Interleukin-1 Receptor-Associated Kinase 4 (p-IRAK4), p65 (p-65), and inhibitor of kappa B alpha (p-IκBα) were detected by western blot as an indication of nuclear factor kappa-B (NF-κB) pathway activation. Results. Our cell and animal experiments revealed that fortunellin exhibits significant anti-inflammatory and cytoprotective properties. Fortunellin counteracted LPS-induced cellular damage in HK-2 cells, enhancing cell survival and suppressing the secretion of pro-inflammatory cytokines. Additionally, fortunellin demonstrated potent antioxidant effects, reducing MDA and Fe2+ levels while increasing SOD activity and GSH content. The protective effect of fortunellin was further corroborated in the mouse model of sepsis-induced AKI. Notably, fortunellin suppressed activation of the TLR4/NF-κB pathway in the AKI model, as evidenced by decreased levels of p-p65 and p-IκBα proteins. Conclusion. Fortunellin ameliorates inflammation and oxidative stress in sepsis-induced AKI, possibly through the modulation of the TLR4/NF-κB pathway. These findings suggest fortunellin's potential as a therapeutic agent for sepsis-associated AKI.
- PublicationOpen AccessExpression and prognostic value of PIM-1 kinase in gliomas(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Li, Zelin; Wang, Hu; Wang, Guangxiu; Wang, Chen; Zhang, Anling; Mo, Lidong; Jia, Zhifan; Tong, Xiaoguang; Biología Celular e HistologíaObjective. This study aimed to explore the correlation of PIM-1 with the clinicopathological features and prognosis of patients. Methods. The MTERF3 mRNA and protein expression levels in tissues were detected by western blot and immunohistochemistry. The expression and survival of PIM-1 in patients with glioma were analysed using the Gene Expression Profiling Interactive Analysis database, the Gene Expression Database of Normal and Tumor Tissues 2, and the Chinese Glioma Genome Atlas database. The relationship between PIM-1 expression and immune cells and chemokines was analysed using the Tumor Immune Estimation Resource Version 2.0 tool and the Tumor and Immune System Interactions Database. A Kaplan–Meier plot was used to estimate the correlation between PIM-1 expression and the survival of patients with glioma. Results. The expression of PIM-1 was upregulated in glioma and was positively correlated with tumour grade. The expression of PIM-1 was significantly inhibited on the second day after transfection (p<0.05), and the inhibition was most obvious on the sixth day (p<0.01). The results of the co-expression pattern of PIM-1 showed that the expression of 5,012 genes was positively correlated with PIM-1, while the expression of 3,651 genes was negatively correlated with PIM-1. Macrophages (p<0.001), myeloid dendritic cells (p<0.001), NK cells (p<0.001), CD4 T cells (p<0.001), cancer-associated fibroblasts (p<0.001), and neutrophils (p<0.001) were positively correlated with the expression of PIM-1 in low-grade glioma. Conclusion. PIM-1 is overexpressed in glioma and is related to the prognosis of glioblastoma multiforme, and PIM-1 may be a prognostic biomarker and therapeutic target for glioma.
- PublicationOpen AccessTherapeutic potential of CD73+ mesenchymal stem cells for myocardial infarction and beyond(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Hou, Huifang; Zheng, Miaoyun; Pan, Kai; Wang, Guodong; Li, Zongjin; Li, Qiong; Biología Celular e HistologíaExtracellular adenine nucleotides serve as crucial signaling molecules and influence a broad spectrum of physiological and pathological processes. CD73, the rate-limiting enzyme in the metabolism of extracellular adenine nucleotides, is ubiquitously expressed on various cell types, particularly stem cells. CD73+ mesenchymal stem cells (MSCs) have emerged as promising candidates for therapeutic applications due to their immunomodulatory and pro-regenerative properties. Numerous studies have highlighted the crucial role of CD73 in mediating tissue protection in myocardial infarction (MI). In this review, a brief overview of the cell type-specific expression, regulatory effects of CD73 on MSCs, and proangiogenic and immunomodulatory mechanisms is provided, with a focus on current findings concerning the protective functions of CD73 in the context of MI within the framework of stem cell therapy
- PublicationOpen AccessIdentification of new tissue markers for the monitoring and standardization of penile cancer according to the degree of differentiation(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Casanova Martín, Carlos; Liviu Boaru, Diego; Fraile Martínez, Oscar; García Montero, Cielo; Leon Oliva, Diego De; Castro Martinez, Patricia De; Gimeno Longas, Maria José; Buján, Julia; García Honduvilla, Natalio; Guijarro, Luis G; Gragera, Raquel; Saez, Miguel A.; Ferrara Coppola, Connie; Baena Romero, Víctor; Diaz Pedrero, Raul; Alvarez Mon, Melchor; Toledo Lobo, M. Val; Ortega, Miguel A.; López González, Laura; Biología Celular e HistologíaPenile cancer is an uncommon disease compared with other urological tumors and is more common in low- and middle-income countries. Risk factors include age, ethnicity, smoking, hygiene, and human papillomavirus infection. Although carcinoma of the penis can be cured in up to 80% of cases if detected early, late diagnosis drastically reduces survival rates, especially in metastatic cases. More than 95% of cases are squamous cell carcinomas, and the degree of cell differentiation is a key histopathological factor, distinguishing between poorly (P), moderately (M), and well-differentiated (W) carcinomas, with verrucous carcinoma (V) having the best prognosis due to its low metastatic capacity. This study analyses the differential expression of several biomarkers related to cell proliferation and cell cycle, inflammation, epigenetics, and autophagy (cell cycle (IRS-4, Ki-67, RB1, CDK4, cyclin D1, ERBB2, β-catenin, and MAGE-A), inflammation (COX2, NLRP3, and AIF-1), epigenetics (HAT-1) and autophagy (ULK-1 and ATG9A) in penile carcinoma according to the degree of differentiation. Immunohistochemical techniques were performed on 34 penile squamous cell carcinoma (PSCC) samples classified into subtype V (N=6), and groups P (N=9), M (N=9), and W (N=10). The findings suggest a differential expression of molecules according to the degree of cell differentiation, with a higher differential expression of molecules according to the degree of cell differentiation, suggesting that the proteins studied could have predictive value. The study highlights the complexity of PSCC and the need for future studies to explore translational applications and search for new biomarkers to improve clinical management and understanding of this disease
- PublicationOpen AccessUSP33 promotes pulmonary microvascular endothelial cell pyroptosis by stabilizing TRAF2 through deubiquitination(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Liang, Jianping; Chen, Junbo; Xu, Pengfei; Biología Celular e HistologíaObjective. Inhibiting the pyroptosis of human pulmonary microvascular endothelial cells (HPMECs) is a promising therapeutic modality for acute lung injury (ALI). Given the undefined effect of ubiquitin-specific protease 33 (USP33) and tumor necrosis factor receptor-associated factor 2 (TRAF2) on pyroptosis in lung injury, this study investigates their roles in the pyroptosis of HPMECs during ALI. Methods. The hypoxia/reoxygenation (H/R)-induced model was constructed in HPMECs. Cell viability, cytotoxicity, and cell death were determined by the cell counting kit-8 (CCK-8), Lactate dehydrogenase (LDH), and Hoechst-PI staining, respectively. Western blot and qRT-PCR were used to detect protein and gene expression levels of pyroptosis-related markers, respectively. The TRAF2 ubiquitination level was measured via immunoprecipitation. Results. USP33 and TRAF2 expressions were elevated in H/R-induced HPMECs. Knockdown of USP33 increased cell viability and inhibited cellular pyroptosis, accompanied by decreases in IL-1β, IL-18, and Caspase-1. USP33 stabilized TRAF2 by deubiquitination. TRAF2 overexpression reversed the effect of USP33 silencing on suppressing HPMEC pyroptosis. Conclusion. USP33 stabilizes TRAF2 by de-ubiquitination to promote HPMEC pyroptosis during ALI.
- PublicationOpen AccessThe potential protective effects of curcumin on the diabetic ovary: Experimental and molecular approaches(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Tufekci, Kıymet Kübra; Altun, Gamze; Kipanyula, Maulilio John; Kaplan, Süleyman; Biología Celular e HistologíaDiabetes mellitus (DM) causes numerous systemic diseases in animals and humans. This may also lead to reproductive problems among individuals of reproductive age. Detrimental effects such as apoptosis in ovarian granulosa cells, degradation of communica-tion proteins, decreased oocyte quality, delayed meiotic maturation, and atrophy are among the increasing evidence that chronic hyperglycemia causes reproductive problems. Numerous studies have reported that the antidiabetic properties of the antioxidant curcumin may be due to its inhibition of oxidative stress, inflammation, and insulin resistance. There are also data indicating that curcumin reduces the risk of DM and its associated symptoms. This review discusses the protective or curative properties of curcumin in the treatment of DM-related problems in the ovary and seeks to elucidate potential underlying mechanisms. While the use of curcumin as a supportive/therapeutic agent has been introduced for the reduction of reproductive problems that may be caused by uncontrolled DM, more studies on this subject are needed.
- PublicationOpen AccessEffects of combustible cigarettes and electronic nicotine delivery systems on the regenerative properties of mesenchymal stem cells derived from periodontal ligament (PDL-MSCs)(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Kastratovic, Nikolina; Milosevic Djordjevic, Olivera; Harrell, Carl Randall; Djonov, Valentin; Volarevic, Vladislav; Biología Celular e HistologíaIntroduction. Periodontal ligament-derived mesenchymal stem cells (PDL-MSCs) are promising cells with crucial roles in maintaining and repairing periodontal tissue. However, their regenerative capacity can be influenced by various factors, including cigarette smoke and electronic nicotine delivery system (ENDS) aerosols. Smoking and vaping can impair their regenerative potential, and even though ENDS are perceived as safer tobacco products, there is a lack of evidence to guarantee this assumption. Material and methods. Changes in the viability and proliferation of PDL-MSCs will be investigated after smoke and aerosol generation and cell exposure. In addition, the effects of smoke and aerosols on the immunomodulatory capacity of PDL-MSCs co-cultured with T lymphocytes will be further determined via the evaluation of cytokine profiles and flow cytometry analysis of T-cell phenotypes. Results. Combustible cigarettes (CCs) induced more severe impairment in the viability and proliferation of PDL-MSCs compared with ENDS. Also, CCs promoted a proinflammatory immune response that could cause tissue damage to progress. On the other hand, ENDS had the potential to generate an immunosuppressive response that would prevent further cell decay. Discussion. The regenerative capacity of PDL-MSCs decreased after treatment with both cigarette smoke and ENDS-aerosols. Even though the results demonstrate less severe effects with ENDS, further research is essential to evaluate their safety and impact on the capacity of PDL-MSCs to prevent and restore oral injuries caused by chronic exposure to aerosols
- PublicationOpen AccessPaeonol regulates glycolytic metabolism by downregulating BACH1 to ameliorate stemness, angiogenesis, and EMT in SiHa cervical cancer cells(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Sheng, Shaoqin; Xu, Jing; Hu, Danhong; Qian, Weiwei; Xu, Xiangqian; He, Jing; Biología Celular e HistologíaAs a common reproductive malignancy of the female reproductive system, cervical cancer has increasingly become a public health concern. Paeonol, which is a natural phenolic monomer, has been found to possess substantial anticancer effects in some human cancers. The present study was conceived to explore the role and mechanism of paeonol in cervical cancer. Initially, the cytotoxicity of paeonol on immortalized H8 cervical epithelial cells and the proliferation of SiHa cervical cancer cells with paeonol treatment were detected using the CCK-8 assay. Cell stemness was assessed with the spheroid formation assay while western blot was applied for the measurement of proteins associated with cell stemness. The tube formation assay was used to detect the angiogenesis of human umbilical vein endothelial cells (HUVECs) and western blot was used to estimate the expression of EMT- and angiogenesis-related proteins. The extra-cellular acidification rate (ECAR) and oxygen consumption rate (OCR) of cells were appraised via a Seahorse XFe24 Flux Analyzer. Lactate production, glucose consumption, and ATP levels were evaluated with corresponding assay kits. Western blot was applied for the evaluation of GLUT1 and HK2. The mRNA and protein expression of BACH1 before and after transfection were detected using RT-qPCR and western blot. The luciferase reporter assay was used to detect the activities of GLUT1 and HK2 promoters. In this study, we found that paeonol inhibited cell proliferation, cell stemness, EMT progress, angiogenesis, and glycolysis in cervical cancer via downregulating BACH1. In summary, paeonol impeded the progression of cervical cancer by regulating glycolytic metabolism through the inhibition of BACH1.