Publication:
An ultracytochemical study on the dynamics of alkaline phosphatase-positive granules in rat neutrophils

Simple item page
dc.contributor.authorJiang, X.
dc.contributor.authorKobayashi, T.
dc.contributor.authorNahirney, P. C.
dc.contributor.authorGarcía del Saz, E.
dc.contributor.authorSeguchi, H.
dc.date.accessioned2020-05-21T16:21:52Z
dc.date.available2020-05-21T16:21:52Z
dc.date.issued1998
dc.description.abstractAlkaline phosphatase (ALPase) activity was examined by cerium-based uitracytochemistry in isolated rat neutrophils following stimulation with phorbol myristate acetate (PMA) or N-formylmethionyl-leucyl-phenylalanine (fMLP). In control neutrophils, low levels of ALPase activity were detected in small tubular and spherical compartments distributed throughout the cytoplasm. Neutrophils stimulated for 2.5, 5, 15, and 30 min with 50 nglml PMA or 10-7 M fMLP displayed a time-dependent increase in ALPase activity. At 2.5 min, an increase in activity was first identified in compartments that were aggregated in the central regions of the cell. By 15 min, a dense precipitate was seen in tubular or elongated bead-like structures that extended to and made contact with the plasma membrane. Large enzyme-positive vacuoles were also observed in regions near the plasma membrane. At the longer stimulation times, a fine precipitate was present on the cell surface of the neutrophil in regions where subplasmalemmal ALPase activity was present. The results of this study indicate that an increase in activity and a redistribution of ALPase-positive structures occurs in neutrophils in response to stimulation with PMA and fMLP. It is likely that these compartments are latent pools of ALPase which, upon stimulation, fuse and mobilize the enzyme activity to the cell surface.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.citationHistology and histopathology, Vol. 13, nº 1 (1998)
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/92462
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectNeutrophiles
dc.subjectAlkaline phosphatasees
dc.subjectUpregulationes
dc.subjectCytochemistryes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleAn ultracytochemical study on the dynamics of alkaline phosphatase-positive granules in rat neutrophilses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
An ultracytochemical study on the dynamics of alkaline phosphatase-positive granules in rat neutr.pdf
Size:
1.52 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.39 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Histology and histopathology Vol.13, nº 1 (1998)