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An ultracytochemical study on the dynamics of alkaline phosphatase-positive granules in rat neutrophils

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An ultracytochemical study on the dynamics of alkaline phosphatase-positive granules in rat neutr.pdf(1.52 MB)
Date
1998
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Authors
Jiang, X. ; Kobayashi, T. ; Nahirney, P. C. ; García del Saz, E. ; Seguchi, H.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Alkaline phosphatase (ALPase) activity was examined by cerium-based uitracytochemistry in isolated rat neutrophils following stimulation with phorbol myristate acetate (PMA) or N-formylmethionyl-leucyl-phenylalanine (fMLP). In control neutrophils, low levels of ALPase activity were detected in small tubular and spherical compartments distributed throughout the cytoplasm. Neutrophils stimulated for 2.5, 5, 15, and 30 min with 50 nglml PMA or 10-7 M fMLP displayed a time-dependent increase in ALPase activity. At 2.5 min, an increase in activity was first identified in compartments that were aggregated in the central regions of the cell. By 15 min, a dense precipitate was seen in tubular or elongated bead-like structures that extended to and made contact with the plasma membrane. Large enzyme-positive vacuoles were also observed in regions near the plasma membrane. At the longer stimulation times, a fine precipitate was present on the cell surface of the neutrophil in regions where subplasmalemmal ALPase activity was present. The results of this study indicate that an increase in activity and a redistribution of ALPase-positive structures occurs in neutrophils in response to stimulation with PMA and fMLP. It is likely that these compartments are latent pools of ALPase which, upon stimulation, fuse and mobilize the enzyme activity to the cell surface.
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Neutrophil , Alkaline phosphatase , Upregulation , Cytochemistry
Citation
Histology and histopathology, Vol. 13, nº 1 (1998)
URI
http://hdl.handle.net/10201/92462
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Histology and histopathology Vol.13, nº 1 (1998)
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