Publication: Production and immunohistochemical characterization of monoclonal antibodies directed against renal basement membranes of rats
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Date
1988
Authors
Hinsch, Klaus-Dieter ; Hansen, Diethelm ; Zimmermann, Astrid ; Bruchhausen, Franz V.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Basement membranes were separated from
rat glomeruli and purified by mild procedures, which led
to a highly enriched basement membrane fraction.
Here, the production and characterization of five
monoclonal antibodies against tubular and glomerular
basement membranes are described. These antibodies
were analyzed immunohistochemically on frozen
sections of rat, bovine, and human kidneys as well as on
rat embryos. One monoclonal antibody (BM 0 11) exclusively
recognized the glomerular basement membranes,
another one (BM 0 VII) bound to tubular basement
membranes and to Bowman's capsule. Three antibodies
(BM 0 IV, BM M 11, BM M 111) recognized their
antigens in both glomerular and tubular basement
membranes as well as in mesangial cells. The BM 0 I1
antibody showed a stringent species specificity and
bound only to glomerular basement membranes of the
rat. The other four antibodies cross-reacted with human
and bovine glomerular basement membrane and
mesangial antigens; they also bound to other tissues in
the developing rat embryo. Antibody binding to specific
purified components of the basement membranes such as
collagen type IV, laminin, heparan sulphate
proteoglycan, and fibronectin was investigated by
enzyme-linked immunosorbent assay (ELISA). None of
these antibodies reacted with any of these known
basement membrane components, indicating that the
antibodies may serve as useful tools in future
investigations of so far unidentified components of
basement membranes.
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