Publication: Murine monoclonal antibodies cytotoxic to human glioma cells in vitro
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Date
1992
Authors
Sánchez-Gómez, M.V. ; Conde Guerri, B. ; Sinués Porta, E. ; Matute, C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Six monoclonal antibodies (mABs) against
human glioma cells (T2) were produced. T2 cells grown
as solid tumors in nude mice, were dissociated and used
to imrnunize Balblc rnice. After fusion of spleenocytes
with myeloma cells, eight hybrids secreting mABs were
selected according to their ability to react immunohistochernically
with T2 cells, but not with normal adult
human brain. Cytotoxicity of mABs was tested using
(3~)-thymidinien corporation assays in vitro. Four mABs
showed complement-mediated cytotoxicity for T2 cells,
other human glioma cells (TI), and a human melanoma
cell line. Incubation with one antibody, mAb2A1,
lowered (3~)-thymidinein corporation in the T2 and T1
cells to ca. lo%, and in melanoma cells to ca. 35% of
control levels. Another antibody, mAb3B2, displayed a
similar cytotoxicity for T2 and T1 cells, but did not
show measurable cytotoxicity for melanoma cells and rat
primary astrocyte cultures. Moreover, this antibody did
not crossreact with haematopoietic cells from patients
bearing CNS tumors or normal subjects. MAb3B2,
therefore, appears to recognize and epitope associated to
human gliomas, will be a useful glioma tumor marker
and may have some potential therapeutical value.
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