Publication: Establishment and in vitro clonal propagation of the Spanish autochthonous table grapevine cultivar Napoleon:an improved system where proliferating cultures alternate
with rooting ones
Authors
Ibáñez-Torres, Ascensión ; Valero, Manuel ; Morte Gómez, María Asunción
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Facultad de Biología
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Publisher
Murcia: Universidad de Murcia, Servicio de Publicaciones
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Ambos medios de cultivo, MS-1 y C2D, con 2 mg/l de 6-benciladenina (BA) dieron los mejores resultados de los seis medios ensayados para el establecimiento in vitro de ápices del cultivar de uva de mesa Napoleón. Se consideraron los parámetros de supervivencia, viabilidad, número de yemas axilares, desarrollo de brotes por explante, longitud del tallo principal y porcentaje de explantes con tallos secundarios. La viabilidad y producción de las yemas y brotes axilares se afectaron seriamente por la época del año en la que se recolectaron las yemas. El estado de desarrollo de las yemas axilares y la posición de los segmentos nodalessobre los brotes en crecimiento tomados de la planta madre infl uenciaron significativamente la respuesta in vitro. La producción de yemas axilares y brotes, y la longitud del tallo principal desarrollado, aumentaron significativamente con la distancia desde el ápice del brote. Se describe un protocolo combinado y secuencial de micropropagación donde alternan cultivos en proliferación de explantes axilares de plántulas crecidas in vitro con cultivos de enraizamiento de los brotes resultantes, y se discute su explotación comercial en la industria viverística.
MS-1 and C2D media both contained 2 mg/l of 6-benzyladenine (BA) led to the best results of six culture media assayed for in vitro establishment of shoot tips from the table grapevine cultivar Napoleon. The parameters considered were survival, viability, number of axillary buds and shoots developing per explant, main shoot length and percentage of explants with secondary shoots. The viability and production of axillary buds and shoots were seriously affected by the time of the year when the buds were collected. The developmental stage of the axillary buds and the position of the nodal segments on the growing shoots taken from the mother plant signifi cantly infl uenced the in vitro response. The production of axillary buds and shoots, and the length of the main developed stem, signifi cantly increased with distance from the shoot tips. A combined and sequential micropropagation protocol where proliferating cultures of axillary-bud microcuttings from in vitro-grown plants alternate with rooting cultures of the resulting shoots is described, and its commercial exploitation by the nursery industry is discussed.
MS-1 and C2D media both contained 2 mg/l of 6-benzyladenine (BA) led to the best results of six culture media assayed for in vitro establishment of shoot tips from the table grapevine cultivar Napoleon. The parameters considered were survival, viability, number of axillary buds and shoots developing per explant, main shoot length and percentage of explants with secondary shoots. The viability and production of axillary buds and shoots were seriously affected by the time of the year when the buds were collected. The developmental stage of the axillary buds and the position of the nodal segments on the growing shoots taken from the mother plant signifi cantly infl uenced the in vitro response. The production of axillary buds and shoots, and the length of the main developed stem, signifi cantly increased with distance from the shoot tips. A combined and sequential micropropagation protocol where proliferating cultures of axillary-bud microcuttings from in vitro-grown plants alternate with rooting cultures of the resulting shoots is described, and its commercial exploitation by the nursery industry is discussed.
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Citation
Ibáñez, A., Valero, M., & Morte, A. (2005). ESTABLECIMIENTO Y PROPAGACIÓN CLONAL IN VITRO DEL CULTIVAR AUTÓCTONO ESPAÑOL DE UVA DE MESA NAPOLEÓN: UN SISTEMA MEJORADO DONDE LOS CULTIVOS EN PROLIFERACIÓN ALTERNAN CON LOS DE ENRAIZAMIENTO. Anales De Biología, (27), 211–220
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