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HBsAg, HBcAg and Delta-Ag in liver tissue: simultaneous visualization in a single tissue section by triple immunostaining

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dc.contributor.authorFacchetti, Fabioes
dc.contributor.authorTardanico, Reginaes
dc.contributor.authorBonetti, Maria F.
dc.contributor.authorGuerini, Agostino
dc.contributor.authorCallea, Francesco
dc.date.accessioned2011-01-17T12:39:06Z
dc.date.available2011-01-17T12:39:06Z
dc.date.issued1986
dc.description.abstractThe distribution pattern of HBsAg, HBcAg and Delta-Ag was investigated by immunohistochemistry in a series of paraffin embedded liver tissue specimens from 45 subjects with serum HBsAg and anti-Delta antibody positivity. An indirect immunoperoxidase technique was used. Stains for HBsAg, HBcAg and Delta-Ag were alternatively carried out on serial tissue sections and, for the first time, consecutively in a single section (triple immunostaining). Simultaneous presence of all antigens occurred in 7 out of 45 cases, and of two antigens (HBsAg and Delta-Ag) in the remaining 38. Two antigens (either HBsAg and HBcAg or HBcAg and Delta-Ag) could also be shown in the same cell. A series of new observations was forwarded by the successful application of triple immunostaining in the present series: 1) high frequence of simultaneous presence of HBcAg and Delta-Ag (7 out of 45 cases = 16%); 2) cytoplasmic localization of Delta-Ag; 3) localization of HBcAg on the cell membrane of HBsAg positive Ground- Glass (G-G) hepatocytes; 4) Ground-Glass appearance of hepatocytic cytoplasm associated with exclusive content of HBcAg (HBcAg-Ground-Glass: a new variant of G-G-hepatocytes).
dc.formatapplication/pdfes
dc.format.extent5es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/17812
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectViral hepatitises
dc.subjectImmunohistochemistryes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleHBsAg, HBcAg and Delta-Ag in liver tissue: simultaneous visualization in a single tissue section by triple immunostaininges
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol. 1, nº 2 (1986)