Publication: HBsAg, HBcAg and Delta-Ag in liver tissue: simultaneous visualization in a single tissue section by triple immunostaining
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Date
1986
Authors
Facchetti, Fabio ; Tardanico, Regina ; Bonetti, Maria F. ; Guerini, Agostino ; Callea, Francesco
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The distribution pattern of HBsAg, HBcAg
and Delta-Ag was investigated by immunohistochemistry
in a series of paraffin embedded liver tissue specimens
from 45 subjects with serum HBsAg and anti-Delta
antibody positivity.
An indirect immunoperoxidase technique was used.
Stains for HBsAg, HBcAg and Delta-Ag were alternatively
carried out on serial tissue sections and, for the first time,
consecutively in a single section (triple immunostaining).
Simultaneous presence of all antigens occurred in 7 out
of 45 cases, and of two antigens (HBsAg and Delta-Ag)
in the remaining 38. Two antigens (either HBsAg and
HBcAg or HBcAg and Delta-Ag) could also be shown in
the same cell.
A series of new observations was forwarded by the
successful application of triple immunostaining in the
present series: 1) high frequence of simultaneous presence
of HBcAg and Delta-Ag (7 out of 45 cases = 16%);
2) cytoplasmic localization of Delta-Ag; 3) localization of
HBcAg on the cell membrane of HBsAg positive Ground-
Glass (G-G) hepatocytes; 4) Ground-Glass appearance
of hepatocytic cytoplasm associated with exclusive content
of HBcAg (HBcAg-Ground-Glass: a new variant of
G-G-hepatocytes).
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