The inhibitory effect of flavonoids and their gut-derived metabolites on the replication of Chlamydia abortus in the AH-1 ovine trophoblast cell line.

Del Río, Laura; Salinas, Jesús; García-Conesa, María Teresa; Buendía Marín, Antonio Julián

Publication:
The inhibitory effect of flavonoids and their gut-derived metabolites on the replication of Chlamydia abortus in the AH-1 ovine trophoblast cell line.

dc.contributor.author	Del Río, Laura
dc.contributor.author	Salinas, Jesús
dc.contributor.author	García-Conesa, María Teresa
dc.contributor.author	Buendía Marín, Antonio Julián
dc.contributor.department	Sanidad Animal
dc.contributor.department	Anatomía y Anatomía Patológica Comparadas
dc.date.accessioned	2024-12-16T11:46:49Z
dc.date.available	2024-12-16T11:46:49Z
dc.date.issued	2019-10-15
dc.description	© 2019 Elsevier. This document is the published version of a published work that appeared in final form in Research in Veterinary Scienc To access the final work, see https://doi.org/10.1016/j.rvsc.2019.09.006
dc.description.abstract	The obligate intracellular bacterium Chlamydia abortus causes abortion and constitutes a worldwide threat for livestock. Plant-derived flavonoids have antimicrobial effects against veterinary and human pathogens and may be of help in the fight against C. abortus. The anti-infective efficacy against C. abortus of the flavonoids apigenin and naringenin, and of four of their derived metabolites was explored using an in vitro model of ovine trophoblast cells. Overall, the anti-infective effectiveness was apigenin > naringenin > benzoic acid > 3-(4- hydroxyphenyl) propionic acid~4-hydroxyphenyl acetic acid~4-hydroxybenzoic. At the lowest concentration tested (10 μM), apigenin, naringenin and benzoic acid inhibited the formation of C. abortus inclusions by 80%, 67%, and 39%, respectively. The cytotoxicity and anti-proliferative effects on the trophoblast host cells also differed greatly between the tested compounds. Our findings suggest that flavonoids may be of therapeutic value against C. abortus infection but metabolic conversion has a substantial and variable effect on their anti-chlamydial activity. Our results also support the notion that the mechanisms of anti-infective action may involve combined effects of the compounds against the host cells and the bacteria.	es
dc.format	application/pdf	es
dc.format.extent	8	es
dc.identifier.doi	https://doi.org/10.1016/j.rvsc.2019.09.006
dc.identifier.eisbn	Research in Veterinary Science 126 (2019) 199–206	es
dc.identifier.issn	0034-5288
dc.identifier.uri	http://hdl.handle.net/10201/147509
dc.language	eng	es
dc.publisher	Elsevier Ltd.	es
dc.relation	Sin financiación externa a la Universidad	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0034528819303595?via%3Dihub	es
dc.rights	info:eu-repo/semantics/restrictedAccess	es
dc.subject	Chlamydiae	es
dc.subject	Natural antibiotics	es
dc.subject	Flavonoids	es
dc.subject	Phenolic metabolites	es
dc.subject	Ruminants	es
dc.subject	Antibacterial	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología	es
dc.title	The inhibitory effect of flavonoids and their gut-derived metabolites on the replication of Chlamydia abortus in the AH-1 ovine trophoblast cell line.	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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relation.isAuthorOfPublication.latestForDiscovery	c084ce85-206c-418d-8cec-7f85a0f34dc0