Publication: Expression of eight genes of nuclear
factor-kappa B pathway in multiple myeloma
using bone marrow aspirates obtained at diagnosis
Loading...
Date
2009
Authors
Sampaio Almeida, Manuella S. ; Vettore, André L. ; Yamamoto, M. ; Chauffaille, Maria de Lourdes L.F. ; Zago, Marco Antônio ; Colleoni, Gisele W. B.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Purpose: To evaluate the expression of NF-
κB pathway genes in total bone marrow samples
obtained from MM at diagnosis using real-time
quantitative PCR and to evaluate its possible correlation
with disease clinical features and survival. Material and
methods: Expression of eight genes related to NF-κB
pathway (NFKB1, IKB, RANK, RANKL, OPG, IL6,
VCAM1 and ICAM1) were studied in 53 bone marrow
samples from newly diagnosed MM patients and in
seven normal controls, using the Taqman system. Genes
were considered overexpressed when tumor expression
level was at least four times higher than that observed in
normal samples. Results: The percentages of
overexpression of the eight genes were: NFKB1 0%, IKB
22.6%, RANK 15.1%, RANKL 31.3%, OPG 7.5%, IL6
39.6%, VCAM1 10% and ICAM1 26%. We found
association between IL6 expression level and
International Staging System (ISS) (p=0.01), meaning
that MM patients with high ISS scores have more chance
of overexpression of IL6. The mean value of ICAM1
relative expression was also associated with the ISS
score (p=0.02). Regarding OS, cases with IL6
overexpression present worse evolution than cases with
IL6 normal expression (p=0.04). Conclusion: We
demonstrated that total bone marrow aspirates can be
used as a source of material for gene expression studies
in MM. In this context, we confirmed that IL6
overexpression was significantly associated with worse
survival and we described that it is associated with high
ISS scores. Also, ICAM1 was overexpressed in 26% of
cases and its level was associated with ISS scores.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.