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Sialoglycoconjugate dimorphism of the mouse submandibular gland acinar cells. Ultrastructural evidence by lectin-protein A-gold probes and sialidase digestion

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dc.contributor.authorMenghi, G.
dc.contributor.authorBondi., A. M.
dc.contributor.authorMarchetti, L.
dc.contributor.authorGabrielli, M. G.
dc.contributor.authorMaterazzi, G.
dc.date.accessioned2020-05-22T12:27:22Z
dc.date.available2020-05-22T12:27:22Z
dc.date.issued1998
dc.description.abstractAn ultrastructural analysis of lectin receptors on th e submandibular glands fro m mice of both sexes was performed utilizing horseradish pero xidase-labell ed lectins in conjunction with antiperox idase antibody and protein A-gold . Both qu alitati ve and quantit ati ve sexrelated differe nces in terminal suga r expression within secre tory gran ul es were detected. Following sia lidase digestion. also suhtenninal acceptor suga rs for terminal siali c ac ids, proved to be differentiall y ex pressed in the submandibular g land s of mal es and fema le s. Heteroge neo us distributi on of sialoglycoconjugates characteri zed bv the terminal disaccharide sialic acid-/3- galactose was f~ llIndt o occur in female ac inar ce lls. Also DBA reacti ve sites indicating the presence of terminal CI.-N-ace tylgalactosamin e discriminated betwee n male and female ac in ar sec re tor y glycoco nju ga tes. This difference was emphasized by sialidase pretreatment that ev idenced a marked occurrence of sialic acid subtended to u- N-ace tvlga lac tosamine in males in contrast to a modest pr es-e ~ce in fe males. The different sial ylation pa tt e rn s of acinar cell sec reto ry prod uc ts, pro bab ly related to a different exp ressio n of 0- and N-linked sia logl ycoconjugates, g iv e in sig ht into th e sex ual dimorphism of the mouse submandibular gland known until rece ntl y for the convoluted granular tubules.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.citationHistology and histopathology, Vol. 13, nº 1 (1998)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/92525
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLectin-gold techniqueses
dc.subjectSialidasees
dc.subjectSubmandibular glandes
dc.subjectMale and female mousees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleSialoglycoconjugate dimorphism of the mouse submandibular gland acinar cells. Ultrastructural evidence by lectin-protein A-gold probes and sialidase digestiones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.13, nº 1 (1998)