Publication:
Sialoglycoconjugate dimorphism of the mouse submandibular gland acinar cells. Ultrastructural evidence by lectin-protein A-gold probes and sialidase digestion

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Date
1998
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Authors
Menghi, G. ; Bondi., A. M. ; Marchetti, L. ; Gabrielli, M. G. ; Materazzi, G.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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Description
Abstract
An ultrastructural analysis of lectin receptors on th e submandibular glands fro m mice of both sexes was performed utilizing horseradish pero xidase-labell ed lectins in conjunction with antiperox idase antibody and protein A-gold . Both qu alitati ve and quantit ati ve sexrelated differe nces in terminal suga r expression within secre tory gran ul es were detected. Following sia lidase digestion. also suhtenninal acceptor suga rs for terminal siali c ac ids, proved to be differentiall y ex pressed in the submandibular g land s of mal es and fema le s. Heteroge neo us distributi on of sialoglycoconjugates characteri zed bv the terminal disaccharide sialic acid-/3- galactose was f~ llIndt o occur in female ac inar ce lls. Also DBA reacti ve sites indicating the presence of terminal CI.-N-ace tylgalactosamin e discriminated betwee n male and female ac in ar sec re tor y glycoco nju ga tes. This difference was emphasized by sialidase pretreatment that ev idenced a marked occurrence of sialic acid subtended to u- N-ace tvlga lac tosamine in males in contrast to a modest pr es-e ~ce in fe males. The different sial ylation pa tt e rn s of acinar cell sec reto ry prod uc ts, pro bab ly related to a different exp ressio n of 0- and N-linked sia logl ycoconjugates, g iv e in sig ht into th e sex ual dimorphism of the mouse submandibular gland known until rece ntl y for the convoluted granular tubules.
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Lectin-gold techniques , Sialidase , Submandibular gland , Male and female mouse
Citation
Histology and histopathology, Vol. 13, nº 1 (1998)
URI
http://hdl.handle.net/10201/92525
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Histology and histopathology Vol.13, nº 1 (1998)
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