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Laser-induced ocular hypertension in adult rats does not affect non-RGC neurons in the ganglion cell layer but results in protracted severe loss of cone-photoreceptors

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dc.contributor.authorOrtín Martínez, Arturo
dc.contributor.authorJiménez López, Manuel
dc.contributor.authorValiente Soriano, Francisco Javier
dc.contributor.authorGarcía Ayuso, Diego
dc.contributor.authorBernal Garro, José Manuel
dc.contributor.authorAvilés Trigueros, Marcelino
dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.authorVillegas Pérez, María Paz
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.authorSalinas Navarro, Manuel Ángel
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.date.accessioned2025-03-26T13:02:47Z
dc.date.available2025-03-26T13:02:47Z
dc.date.issued2015-01-07
dc.description© 2015 The Authors. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published Manuscript, version of a Published Work that appeared in final form in Experimental Eye Research. To access the final edited and published work see https://doi.org/10.1016/j.exer.2015.01.006es
dc.description.abstractTo investigate the long-term effects of laser-photocoagulation (LP)-induced ocular hypertension (OHT) in the innermost and outermost (outer-nuclear and outer segment)-retinal layers (ORL). OHT was induced in the left eye of adult rats. To investigate the ganglion cell layer (GCL) wholemounts were examined at 1, 3 or 6 months using Brn3a-immunodetection to identify retinal ganglion cells (RGCs) and DAPI-staining to detect all nuclei in this layer. To study the effects of LP on the ORL up to 6 months, retinas were: i) fresh extracted to quantify the levels of rod-, S- and L-opsin; ii) cut in cross-sections for morphometric analysis, or; iii) prepared as wholemounts to quantify and study retinal distributions of entire populations of RGCs (retrogradely labeled with fluorogold, FG), S- and L-cones (immunolabeled). OHT resulted in wedge-like sectors with their apex on the optic disc devoid of Brn3a+RGCs but with large numbers of DAPI+nuclei. The levels of all opsins diminished by 2 weeks and further decreased to 20% of basal-levels by 3 months. Cross-sections revealed focal areas of ORL degeneration. RGC survival at 15 days represented approximately 28% and did not change with time, whereas the S- and L-cone populations diminished to 65% and 80%, or to 20 and 35% at 1 or 6 months, respectively. In conclusion, LP induces in the GCL selective RGCs loss that does not progress after 1 month, and S- and L-cone loss that progresses for up to 6 months. Thus, OHT results in severe damage to both the innermost and the ORL.es
dc.formatapplication/pdfes
dc.format.extent17es
dc.identifier.citationExperimental Eye Research, 2015, Vol. 132, pp. 17-33
dc.identifier.doihttps://doi.org/10.1016/j.exer.2015.01.006
dc.identifier.issnPrint: 0014-4835
dc.identifier.urihttp://hdl.handle.net/10201/152180
dc.languageenges
dc.publisherElsevieres
dc.relationSpanish Ministry of Economy and Competitiveness: SAF-2012-38328; ISCIII-FEDER “Una manera de hacer Europa” PI13/00643, PI13/01266, RETICS: RD12/0034/0014.es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S001448351500007Xes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectOcular hypertensiones
dc.subjectAdult albino rat retinal ganglion cellses
dc.subjectNeuronal degenerationes
dc.subjectS cones L coneses
dc.subjectPhotoreceptors losses
dc.subjectDisplaced amacrine cellses
dc.subjectGanglion cell layer of the retinaes
dc.subjectOuter retinal layerses
dc.subjectElevated intraocular pressurees
dc.subjectLimbar laser photocoagulationes
dc.subjectRodent experimental glaucoma modeles
dc.titleLaser-induced ocular hypertension in adult rats does not affect non-RGC neurons in the ganglion cell layer but results in protracted severe loss of cone-photoreceptorses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication81da925b-fa46-4a1a-96fd-ce35568fa423
relation.isAuthorOfPublicationba9bf053-48ec-4e39-93ac-ffcbaf10fc0f
relation.isAuthorOfPublication.latestForDiscovery81da925b-fa46-4a1a-96fd-ce35568fa423
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