Publication: Laser-induced ocular hypertension in adult rats does not affect non-RGC neurons in the ganglion cell layer but results in protracted severe loss of cone-photoreceptors
Authors
Ortín Martínez, Arturo ; Jiménez López, Manuel ; Valiente Soriano, Francisco Javier ; García Ayuso, Diego ; Bernal Garro, José Manuel ; Avilés Trigueros, Marcelino ; Agudo Barriuso, Marta ; Villegas Pérez, María Paz ; Vidal Sanz, Manuel ; Salinas Navarro, Manuel Ángel ; Nadal-Nicolás, Francisco Manuel
item.page.secondaryauthor
item.page.director
Publisher
Elsevier
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1016/j.exer.2015.01.006
item.page.type
info:eu-repo/semantics/article
Description
© 2015 The Authors. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Published Manuscript, version of a Published Work that appeared in final form in Experimental Eye Research. To access the final edited and published work see https://doi.org/10.1016/j.exer.2015.01.006
Abstract
To investigate the long-term effects of laser-photocoagulation (LP)-induced ocular hypertension (OHT) in the innermost and outermost (outer-nuclear and outer segment)-retinal layers (ORL). OHT was induced in the left eye of adult rats. To investigate the ganglion cell layer (GCL) wholemounts were examined at 1, 3 or 6 months using Brn3a-immunodetection to identify retinal ganglion cells (RGCs) and DAPI-staining to detect all nuclei in this layer. To study the effects of LP on the ORL up to 6 months, retinas were: i) fresh extracted to quantify the levels of rod-, S- and L-opsin; ii) cut in cross-sections for morphometric analysis, or; iii) prepared as wholemounts to quantify and study retinal distributions of entire populations of RGCs (retrogradely labeled with fluorogold, FG), S- and L-cones (immunolabeled). OHT resulted in wedge-like sectors with their apex on the optic disc devoid of Brn3a+RGCs but with large numbers of DAPI+nuclei. The levels of all opsins diminished by 2 weeks and further decreased to 20% of basal-levels by 3 months. Cross-sections revealed focal areas of ORL degeneration. RGC survival at 15 days represented approximately 28% and did not change with time, whereas the S- and L-cone populations diminished to 65% and 80%, or to 20 and 35% at 1 or 6 months, respectively. In conclusion, LP induces in the GCL selective RGCs loss that does not progress after 1 month, and S- and L-cone loss that progresses for up to 6 months. Thus, OHT results in severe damage to both the innermost and the ORL.
publication.page.subject
Ocular hypertension , Adult albino rat retinal ganglion cells , Neuronal degeneration , S cones L cones , Photoreceptors loss , Displaced amacrine cells , Ganglion cell layer of the retina , Outer retinal layers , Elevated intraocular pressure , Limbar laser photocoagulation , Rodent experimental glaucoma model
Citation
Experimental Eye Research, 2015, Vol. 132, pp. 17-33
item.page.embargo
Collections
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/